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1.

001-es BibID:BIBFORM118494
035-os BibID:(cikkazonosító)535 (WoS)000643797400001 (Scopus)85103862538
Első szerző:Bali Krisztina
Cím:Recombination Events Shape the Genomic Evolution of Infectious Bronchitis Virus in Europe / Krisztina Bali, Ádám Bálint, Attila Farsang, Szilvia Marton, Borbála Nagy, Eszter Kaszab, Sándor Belák, Vilmos Palya, Krisztián Bányai
Dátum:2021
ISSN:1999-4915
Megjegyzések:Infectious bronchitis of chicken is a high morbidity and mortality viral disease affecting the poultry industry worldwide; therefore, a better understanding of this pathogen is of utmost importance. The primary aim of this study was to obtain a deeper insight into the genomic diversity of field infectious bronchitis virus (IBV) strains using phylogenetic and recombination analysis. We sequenced the genome of 20 randomly selected strains from seven European countries. After sequencing, we created a genome sequence data set that contained 36 European origin field isolates and 33 vaccine strains. When analyzing these 69 IBV genome sequences, we identified 215 recombination events highlighting that some strains had multiple recombination breaking points. Recombination hot spots were identified mostly in the regions coding for non-structural proteins, and multiple recombination hot spots were identified in the nsp2, nsp3, nsp8, and nsp12 coding regions. Recombination occurred among different IBV genotypes and involved both field and vaccine IBV strains. Ninety percent of field strains and nearly half of vaccine strains showed evidence of recombination. Despite the low number and the scattered geographical and temporal origin of whole-genome sequence data collected from European Gammacoronaviruses, this study underlines the importance of recombination as a major evolutionary mechanism of IBVs.
Tárgyszavak:Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Megjelenés:Viruses-Basel. - 13 : 4 (2021), p. 1-13. -
További szerzők:Bálint Ádám Farsang Attila Marton Szilvia Nagy Borbála Kaszab Eszter (1989-) (biológus) Belák Sándor Palya Vilmos Bányai Krisztián
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2.

001-es BibID:BIBFORM118497
035-os BibID:(WoS)000690213000001 (Scopus)85112026538 (cikkazonosító)849
Első szerző:Bálint Ádám
Cím:Genetic Variability of PRRSV Vaccine Strains Used in the National Eradication Programme, Hungary / Ádám Bálint,Tamás Molnár, Sándor Kecskeméti, Gábor Kulcsár, Tibor Soós, Péter M. Szabó, Eszter Kaszab, Kinga Fornyos, Zoltán Zádori, Krisztián Bányai, István Szabó
Dátum:2021
ISSN:2076-393X
Megjegyzések:Porcine reproductive and respiratory syndrome (PRRS) is a globally spread, highly infectious viral disease. Live, attenuated vaccines against PRRS virus (PRRSV) decrease virus excretion and evoke protective immunity reducing the economic damage caused by the disease. In a longitudinal molecular epidemiological study accompanying ongoing national eradication programme we evaluated the suitability of PRRSV ORF5 and ORF7 sequences to identify possible field strains of vaccine-origin. In total, 2342 ORF5 sequences and 478 ORF7 sequences were analysed. Vaccine strains were identified by sequence identity values and phylogenetic network analysis. Strains that shared greater than 98% nucleotide identity within ORF5 and/or ORF7 were considered to have originated from vaccine. A total of 882 (37.6%) ORF5 and 88 (18.4%) ORF7 sequences met these criteria. In detail, 618, 179 and 35 ORF5 and 51, 29 and 8 ORF7 sequences were related to Porcilis PRRS vaccine, Unistrain PRRS vaccine, and ReproCyc PRRS EU vaccine, respectively. Data showed that the Porcilis vaccine was genetically more stable. Whereas, the variability of the Unistrain and the ReproCyc strains was significantly higher. Given that ORF7 shares, in some instances, complete identity between a particular vaccine strain and some historic variants of field PRRSV strains, care must be taken when evaluating vaccine relatedness of a field isolate based on the ORF7. On the contrary, ORF5 sequences were more suitable to predict the vaccine origin making a distinction more robustly between field and vaccine strains. We conclude that ORF5 based molecular epidemiological studies support more efficiently the ongoing PRRS eradication programmes. The conclusions presented in this large-scale PRRS molecular epidemiological study provides a framework for future eradication programmes planned in other countries.
Tárgyszavak:Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Megjelenés:Vaccines. - 9 : 8 (2021), p. 1-12. -
További szerzők:Molnár Tamás Kecskeméti Sándor Kulcsár Gábor Soós Zoltán Szabó Péter Kaszab Eszter (1989-) (biológus) Fornyos Kinga Zádori Zoltán Bányai Krisztián Szabó István
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3.

001-es BibID:BIBFORM117940
035-os BibID:(cikkazonosító)175 (WoS)001139768900001 (Scopus)85181965425
Első szerző:Bálint Ádám
Cím:Spatiotemporal Distribution of PRRSV-1 Clades in Hungary with a Focus on the Era of Disease Eradication / Ádám Bálint, Szilvia Jakab, Eszter Kaszab, Szilvia Marton, Krisztián Bányai, Sándor Kecskeméti, István Szabó
Dátum:2024
ISSN:2076-2615
Megjegyzések:Porcine reproductive and respiratory syndrome (PRRS) is the cause of the most severe economic losses in the pig industry worldwide. PRRSV is extremely diverse in Europe, which poses a significant challenge to disease control within a country or any region. With the combination of phylogenetic reconstruction and network analysis, we aimed to uncover the major routes of the dispersal of PRRSV clades within Hungary. In brief, by analyzing >2600 ORF5 sequences, we identified at least 12 clades (including 6 clades within lineage 1 and 3 clades within lineage 3) common in parts of Western Europe (including Denmark, Germany and the Netherlands) and identified 2 novel clades (designated X1 and X2). Of interest, some genetic clades unique to other central European countries, such as the Czech Republic and Poland, were not identified. The pattern of PRRSV clade distribution is consistent with the route of the pig trade among countries, showing that most of the identified clades were introduced from Western Europe when fatteners were transported to Hungary. As a result of rigorous implementation of the national eradication program, the swine population was declared officially free from PRRSV. This map of viral diversity and clade distribution will serve as valuable baseline information for the maintenance of PRRSV-free status in the post-eradication era.
Tárgyszavak:Orvostudományok Egészségtudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
disease eradication
genetic diversity
molecular epidemiology
phylogeny
porcine reproductive and respiratory syndrome
PRRS
Megjelenés:Animals. - 14 : 1 (2024), p. 1-8. -
További szerzők:Jakab Szilvia Kaszab Eszter (1989-) (biológus) Marton Szilvia Bányai Krisztián Kecskeméti Sándor Szabó István
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4.

001-es BibID:BIBFORM118498
035-os BibID:(Cikkazonosító)1592 (WoS)000690239600001 (Scopus)85113142544
Első szerző:Fehér Enikő (molekuláris biológus, mikrobiológus)
Cím:Genomic Epidemiology and Evolution of Duck Hepatitis A Virus / Enikő Fehér, Szilvia Jakab, Krisztina Bali, Eszter Kaszab, Borbála Nagy, Katalin Ihász, Ádám Bálint, Vilmos Palya, Krisztián Bányai
Dátum:2021
ISSN:1999-4915
Megjegyzések:Duck hepatitis A virus (DHAV), an avian picornavirus, causes high-mortality acute disease in ducklings. Among the three serotypes, DHAV-1 is globally distributed, whereas DHAV-2 and DHAV-3 serotypes are chiefly restricted to Southeast Asia. In this study, we analyzed the genomic evolution of DHAV-1 strains using extant GenBank records and genomic sequences of 10 DHAV-1 strains originating from a large disease outbreak in 2004?2005, in Hungary. Recombination analysis revealed intragenotype recombination within DHAV-1 as well as intergenotype recombination events involving DHAV-1 and DHAV-3 strains. The intergenotype recombination occurred in the VP0 region. Diversifying selection seems to act at sites of certain genomic regions. Calculations estimated slightly lower rates of evolution of DHAV-1 (mean rates for individual protein coding regions, 5.6286 ? 10?4 to 1.1147 ? 10?3 substitutions per site per year) compared to other picornaviruses. The observed evolutionary mechanisms indicate that whole-genome-based analysis of DHAV strains is needed to better understand the emergence of novel strains and their geographical dispersal.
Tárgyszavak:Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Megjelenés:Viruses-Basel. - 13 : 8 (2021), p. 1-10. -
További szerzők:Jakab Szilvia Bali Krisztina Kaszab Eszter (1989-) (biológus) Nagy Borbála Ihász Katalin Bálint Ádám Palya Vilmos Bányai Krisztián
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5.

001-es BibID:BIBFORM118513
035-os BibID:(WoS)000898136200001 (cikkazonosító)1093884 (Scopus)85143892810
Első szerző:Jakab Szilvia
Cím:Corrigendum: Genetic diversity of imported PRRSV-2 strains, 2005-2020, Hungary / Szilvia Jakab, Eszter Kaszab, Szilvia Marton, Krisztián Bányai, Ádám Bálint, Imre Nemes, István Szabó
Dátum:2022
ISSN:2297-1769
Megjegyzések:Porcine reproductive and respiratory syndrome virus 2 (PRRSV-2) remains sporadic in Europe. In this study, we investigated the molecular epidemiology of PRRSV-2 infections encompassing 15 years in Hungary. Partial (423 bp long) ORF5 sequences (n = 44) from 20 Hungarian pig herds were analyzed. The study strains fell into two genetic lineages, L1 and L5, being L5 strains more prevalent (88.6 vs. 11.4%). Pairwise sequence identities within Hungarian representative PRRSV-2 strains ranged between 84.7 to 100% (nucleotide, nt) and 85 to 100% (amino acid, aa). When compared with reference strains, identity values fell between 87 and 100% (L1, nt 87?91%, aa 87?93%, reference strain IAF-exp91; L5, nt 87?100%, aa 88?100%, reference strain Ingelvac MLV). Epidemiologic examination implied that the majority of L5 strains were imported repeatedly from other European countries where Ingelvac MLV was approved for routine use. The emergence of L1 strains was thought to be associated with a single introduction and subsequent dissemination between pig farms of a large integrator. Results presented here contribute to a better understanding of the epizootiology of PRRSV-2 infections and shed light on the genetic diversity of viral strains in non-endemic countries.
Tárgyszavak:Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Megjelenés:Frontiers in Veterinary Science. - 9 (2022), p. 1093884. -
További szerzők:Kaszab Eszter (1989-) (biológus) Marton Szilvia Bányai Krisztián Bálint Ádám Nemes Imre Szabó István
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6.

001-es BibID:BIBFORM118507
035-os BibID:(WoS)000876193300001 (Scopus)85140402684 (cikkazonosító)1093884
Első szerző:Jakab Szilvia
Cím:Genetic diversity of imported PRRSV-2 strains, 2005-2020, Hungary / Szilvia Jakab, Eszter Kaszab, Szilvia Marton, Krisztián Bányai, Ádám Bálint, Imre Nemes, István Szabó
Dátum:2022
ISSN:2297-1769
Megjegyzések:Porcine reproductive and respiratory syndrome virus 2 (PRRSV-2) remains sporadic in Europe. In this study, we investigated the molecular epidemiology of PRRSV-2 infections encompassing 15 years in Hungary. Partial (423 bp long) ORF5 sequences (n = 44) from 20 Hungarian pig herds were analyzed. The study strains fell into two genetic lineages, L1 and L5, being L5 strains more prevalent (88.6 vs. 11.4%). Pairwise sequence identities within Hungarian representative PRRSV-2 strains ranged between 84.7 to 100% (nucleotide, nt) and 85 to 100% (amino acid, aa). When compared with reference strains, identity values fell between 87 and 100% (L1, nt 87-91%, aa 87-93%, reference strain IAF-exp91; L5, nt 87-100%, aa 88-100%, reference strain Ingelvac MLV). Epidemiologic examination implied that the majority of L5 strains were imported repeatedly from other European countries where Ingelvac MLV was approved for routine use. The emergence of L1 strains was thought to be associated with a single introduction and subsequent dissemination between pig farms of a large integrator. Results presented here contribute to a better understanding of the epizootiology of PRRSV-2 infections and shed light on the genetic diversity of viral strains in non-endemic countries.
Tárgyszavak:Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Megjelenés:Frontiers in Veterinary Science. - 9 (2022), p. 1-10. -
További szerzők:Kaszab Eszter (1989-) (biológus) Marton Szilvia Bányai Krisztián Bálint Ádám Nemes Imre Szabó István
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7.

001-es BibID:BIBFORM118255
035-os BibID:(cikkazonosító)1327725 (Scopus)85182813583
Első szerző:Jakab Szilvia
Cím:Genome stability assessment of PRRS vaccine strain with new ARTIC-style sequencing protocol / Szilvia Jakab, Ádám Bálint, Karolina Cseri, Krisztina Bali, Eszter Kaszab, Marianna Domán, Máté Halas, Krisztina Szarka, Krisztián Bányai
Dátum:2024
ISSN:2297-1769
Megjegyzések:A tiling amplicon sequencing protocol was developed to analyse the genome sequence stability of the modified live PRRSV vaccine strain, Porcilis MLV. The backbone of the ARTIC-style protocol was formed by 34 individual primer pairs, which were divided into two primer pools. Primer pairs were designed to amplify 532 to 588 bp fragments of the corresponding genomic region. The amplicons are suitable for sequencing on Illumina DNA sequencers with available 600-cycle sequencing kits. The concentration of primer pairs in the pools was optimized to obtain a balanced sequencing depth along the genome. Deep sequencing data of three vaccine batches were also analysed. All three vaccine batches were very similar to each other, although they also showed single nucleotide variations (SNVs) affecting less than 1 % of the genome. In the three vaccine strains, 113 to 122 SNV sites were identified; at these sites, the minority variants represented a frequency range of 1 to 48.7 percent. Additionally, the strains within the batches contained well-known length polymorphisms; the genomes of these minority deletion mutants were 135 to 222 bp shorter than the variant with the complete genome. Our results show the usefulness of ARTIC-style protocols in the evaluation of the genomic stability of PRRS MLV strains.
Tárgyszavak:Orvostudományok Egészségtudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
deep sequencing
genetic variability
Porcilis MLV
porcine reproductive and respiratory syndrome virus
single nucleotide variation
tiling amplicon sequencing
Megjelenés:Frontiers in Veterinary Science. - 10 (2024), p. 1-8. -
További szerzők:Bálint Ádám Cseri Karolina (1985-) (molekuláris biológus) Bali Krisztina Kaszab Eszter (1989-) (biológus) Domán Marianna (1987-) (biológus) Halas Máté Szarka Krisztina (1971-) (molekuláris biológus, mikrobiológus) Bányai Krisztián
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8.

001-es BibID:BIBFORM118514
035-os BibID:(WoS)000685293500003
Első szerző:Kaszab Eszter (biológus)
Cím:Víziszárnyasok polyomavírus-fertőzése / Kaszab Eszter, Bali Krisztina, Bálint Ádám, Süli Tamás, Bajnóczi Pál, Bányai Krisztián, Fehér Enikő
Dátum:2021
ISSN:0025-004X
Megjegyzések:Goose haemorrhagic polyomavirus (GHPV) infection may induce fulminant disease and death of young geese causing serious economic losses for farmers. The review summarizes the pathological and histopathological characteristics of the disease and the characteristics of the virus. GHPV DNA has been identified in the internal organs of different wild bird species that may imply effective virus replication in a number of heterologous hosts and the role of those in the maintenance of viral circulation in nature. The analysis of viral genomic sequences during a retrospective molecular epidemiological study revealed co-circulating GHPV variants in goose flocks that may be the consequence of mutations in the genome of persisting viruses, or introduction and re-introduction of various strains. The wide host range, the persistent infection, the environmental contamination and the trade of live birds and animal products may facilitate the transmission of GHPV. The virus has a relatively conserved genome and capsid proteins, a feature that may be advantageous for vaccine development against the disease.
Tárgyszavak:Természettudományok Biológiai tudományok magyar nyelvű folyóiratközlemény hazai lapban
folyóiratcikk
Megjelenés:Magyar Allatorvosok Lapja. - 143 : 7 (2021), p. 401-408. -
További szerzők:Bali Krisztina Bálint Ádám Süli Tamás Bajnóczi Pál Bányai Krisztián Fehér Enikő (1981-) (molekuláris biológus, mikrobiológus)
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Intézményi repozitóriumban (DEA) tárolt változat
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9.

001-es BibID:BIBFORM118491
035-os BibID:(WoS)000536901100001 (Scopus)85085869043
Első szerző:Kaszab Eszter (biológus)
Cím:Molecular epidemiology and phylodynamics of goose haemorrhagic polyomavirus / Eszter Kaszab, Szilvia Marton, Ádám Dán, Attila Farsang, Ádám Bálint, Krisztián Bányai, Enikő Fehér
Dátum:2020
ISSN:1865-1674 1865-1682
Tárgyszavak:Természettudományok Biológiai tudományok magyar nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Megjelenés:Transboundary and Emerging Diseases. - 67 : 6 (2020), p. 2602-2608. -
További szerzők:Marton Szilvia Dán Ádám Farsang Attila Bálint Ádám Bányai Krisztián Fehér Enikő (1981-) (molekuláris biológus, mikrobiológus)
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