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001-es BibID:	BIBFORM114860
Első szerző:	Műzes Györgyi
Cím:	IGF1R inhibition affects the survival of HT29 cancer cells by alterations of the TLR9- and autophagy signaling / G. Műzes, A. Sebestyén, Á. Simon, L. Nagy, B. Barta, T. Dankó, A. L. Kiss, F. J. Sipos
Dátum:	2019
ISSN:	0923-7534 1569-8041
Megjegyzések:	Background In HT29 cells, an interplay between self-DNA-induced TLR9-signaling and autophagy response was found with significant effects on cell survival and kinetic parameters. The IGF/IGF1R system plays a determinant role in the pathogenesis and progression of colorectal cancer. This pathway is upstream of mTORC1, a negative regulator of autophagy. I mammalian systems chronic IGF1R inhibition was shown to attenuate autophagosome formation. The interrelated action of IGF1R inhibition and TLR9/autophagy signaling in cancer cells has not yet been clarified. The present study was designed to assess this complex network using HT29 colon carcinoma cells. Methods HT29 cells were incubated for 72 h with genomic (g), hypermethylated (m), and fragmented (f) tumor self-DNAs, and with/without inhibitors of IGF1R (picropodophyllin), autophagy (chloroquine) and TLR9 (ODN2088), respectively. Cell viability was measured by MTT assay. Transcriptional changes of TLR9-signaling, IGF1R, mTORC, Akt, and the autophagy process were assayed by Human v3 miRNA Assay (NanoString). Autophagy proteins were detected by immunocytochemistry, while morphology of apoptosis and autophagy by transmission electron microscopy (TEM). Results In case of autophagy g- and f-DNAs caused significant upregulation of Beclin1, Atg16L1, LC3 mRNAs, and downregulation of mTORC, and Akt, verified by immunocytochemistry, as well. IGF1R-inhibition alone altered inversely the autophagy-associated gene- and protein-expressions. Upon combined inhibition of autophagy, TLR9 and/or IGF1R-signaling varying degree of autophagy was detected in all groups of tumor cells according to NanoString and TEM. Incubation with IGF1R inhibitor and with m-DNA no expected suppression of tumor cell survival, induction of apoptotic death, and activation of mitophagy were found. Moreover, IGF1R inhibition affected negatively the cell-protective effect f-DNA on macroautophagy and lipophagy. Conclusions Our study provided evidence for an interaction between the inhibited IGF1R and TLR9/autophagy signaling with major influences on survival, proliferation and death of HT29 cells subjected to intact/modified self-DNAs. (Funded by StartUp.). Legal entity responsible for the study Ferenc Sipos. Funding StartUp. Disclosure All authors have declared no conflicts of interest.
Tárgyszavak:	Orvostudományok Klinikai orvostudományok idézhető absztrakt folyóiratcikk
Megjelenés:	Annals Of Oncology. - 30 : Suppl_5 (2019), p. 806-807. -
További szerzők:	Sebestyén Andor Simon Ágnes Nagy Lőrinc (1995-) (biológus, gyógyszer-biotechnológus) Barta Bianka Danko Tamás Kiss A. L. Sipos F. J.
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001-es BibID:	BIBFORM114861
Első szerző:	Sipos F. J.
Cím:	Modulation of TLR9-dependent autophagy response via inhibition of c-Met signaling influences the survival of HT29 cancer cells / F. J. Sipos, L. Nagy, B. Barta, Á. Simon, T. Dankó, A. Sebestyén, A. L. Kiss, G. Műzes
Dátum:	2019
ISSN:	0923-7534 1569-8041
Megjegyzések:	Background:InHT29cells,aninterplaybetweenself-DNA-inducedTLR9-and autophagyresponseswasfoundwithremarkableeffectsonsurvivalanddifferentiation oftumorcells.c-Metactivationisknowntodrivetheprogressionofcolorectalcancer bypromotingsignalingcascadesthatmainlyresultinalterationsofcellmotility,survivalandproliferation.c-Metinhibitionwasshowntoinhibitautophagy.Incancer cellstheinterrelatedroleofc-MetinhibitionandTLR9/autophagysignalinghasnotyet beenclarified,soweaimedtoassessthiscomplexinterplay. Methods:HT29cellswereincubatedfor72hwithgenomic(g),hypermethylated(m), andfragmented(f)tumorself-DNAs,andwith/withoutinhibitorsofc-Met(diisothiocyanatostilbene),autophagy(chloroquine)andTLR9(ODN2088),respectively.Cell viabilitywasmeasuredbyMTTassay.TranscriptionalchangesofTLR9-signaling, PI3K,CD95,c-Met,Bcl2,cytochrome-c,andtheautophagyprocesswereassayedby Humanv3miRNAAssay(NanoString).Autophagyproteinsweredetectedbyimmunocytochemistry,whilemorphologyofapoptosisandautophagybytransmissionelectronmicroscopy(TEM). Results:Self-DNAsgandfresultedinsignificantupregulationofBeclin1,Atg16L1, LC3mRNAs,anddownregulationofPI3K,Bcl2,CD95,andcytochrome-c,verifiedby immunocytochemistry,aswell.c-Metinhibitionalonealteredinverselytheautophagyassociatedgene-andprotein-expressions.Ineachgroupoftumorcellsusingcombined inhibitionofautophagy,TLR9and/orc-Met-signalingvaryingdegreeofautophagy wasobservedaccordingtoNanoStringandTEM.Followingcombinedincubationwith c-Metinhibitorandm-DNAsnoexpectedsuppressionoftumorcellsurvivaland inductionofapoptosisandmitophagyweredetected.Further,c-Metinhibition changedthecell-protectiveeffectf-DNAonmacroautophagy. Conclusions:Ourstudyprovidedevidenceforanintensecrosstalkbetweentheinhibitedc-Metcanonicalandnon-canonicalsignalingpathways,andtheTLR9/autophagy responsewithprofoundimpactsonsurvival,proliferationanddeathofHT29cellssubjectedtointact/modifiedself-DNAs. Legalentityresponsibleforthestudy:FerencSipos. Funding:StartUp. Disclosure:Allauthorshavedeclarednoconflictsofinterest.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idézhető absztrakt folyóiratcikk
Megjelenés:	Annals Of Oncology. - 30 : Suppl_5 (2019), p. v807. -
További szerzők:	Nagy Lőrinc (1995-) (biológus, gyógyszer-biotechnológus) Barta Bianka Simon Ágnes Danko Tamás Sebestyén Andor Kiss A. L. Műzes Györgyi
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