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001-es BibID:	BIBFORM119838
Első szerző:	Elshafia, Ali Hamid Mohammed agrármérnök
Cím:	Survey on antibiotic resistant bacteria in commercial fish ponds / Elshafia Ali Hamid Mohammed; Renáta Bőkényné Tóth; Gábor Kardos; Csongor Freytag; Károly Pál
Dátum:	2024
Megjegyzések:	Fish is a valuable source of proteins and commercial fish farms help to satisfy the demand. Hungarian farms produce a large amount of fresh fish every year, in many lakes countrywide. Due to the fish farming activities (the use of antibiotics for curing fish with bacterial infections) and the migrating birds (reservoir of pathogenic microbes), these lakes may be sources of pathogenic bacteria that confer antibiotic resistance. These bacteria may pose a threat not only to the fish but employees and consumers, as well. A joint project of the Institute of Metagenomics and the microbiology laboratory of the Institute of Food Sciences is to perform regular surveys on the microbiological quality of fish farm lake water, with emphasis on the detection of possible AMR bacteria, in accordance with the Farm to Fork Strategy. The survey started in 2023 and 10-10 lakes were sampled in two fish farms, respectively. After filtration of water samples, bacteria were isolated on vancomycine and Eosine Methylene Blue agar plates, then identified by MALDI-TOF. Though, numerous bacterial species were found, no traces of vancomycine resistant Enterococcus isolates were found yet.
Tárgyszavak:	Agrártudományok Állattenyésztési tudományok előadáskivonat könyvrészlet
Megjelenés:	1st Debrecen Online Conference on Infectious Diseases in a One Health context (DOCIDOH) : Book of abstracts. - p. 12
További szerzők:	Bőkényné Tóth Renáta Kardos Gábor (1974-) (absztraktok) Freytag Csongor (1993-) (biológus) Pál Károly (1978-) (mikrobiológus)
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001-es BibID:	BIBFORM119720
Első szerző:	Elshafia, Ali Hamid Mohammed agrármérnök
Cím:	Identification of Antibiotic-Resistant Bacteria in Commercial Fish Farms in Hungary / Elshafia Ali Hamid Mohammed; Renáta Bőkényné Tóth; Gábor Kardos; Pál Károly
Dátum:	2024
Megjegyzések:	The common carp (Cyprinus carpio) is a popular species in fish farms in Hungary (Europe). Since Hungary is a member state of the European Union, the use of antibiotics for growth promotion of animals has been banned since 2011. An even stricter legislation came into force in 2022 to inhibit the spread of antibiotic resistant bacteria in animals and humans, in accordance with the Farm to Fork Strategy. Nevertheless, new strains of AMR conferring bacteria may appear in fish farm lakes as a result of antibiotic treatment of bacterial infections, or due to wild birds as reservoirs. Antibiotic resistance genes (especially in form of mobile genetic elements, e.g. plasmids) may pose a serious threat to the fish production, health of employees, and environment. The current study aimed to survey the occurrence of antibiotic resistant bacteria and confirm their identity in water samples of commercial fish farms in Hungary. Twenty water samples were collected from two fish farms and subjected to bacterial isolation using a membrane filtration technique (pore size: 0.45-micrometer). Then the membranes were placed on different types of selected media (VRE, Eosin Methylene Blue and Endo's Medium). Bacterial colonies were subjected to matrix-assisted laser desorption ionization?timeof- flight mass spectrometry (MALDI-TOF MS) technique for species level identification. The following species were detected: Acinetobacter baumannii, A. calcoacetius, A. junii, A. pittii, A. tandoii, Bacillus pumilus, Comamonas aquatica, Enterococcus casseliflavus, E. gallinarum, Escherichia coli, Lactobacillus plantarum, Ochrobactrum intermedium, Pediococcus pentosaceus, Pseudomonas aeruginosa, P. alcaligenes, P. mendocina, P. otidis, P. putida and Stenotrophomonas maltophilia. In the next step, we will use antibiotic tests to investigate, whether these isolates have some unusual extrinsic antibiotic resistance. In addition, we will perform PCR experiments to detect the presence of virulence factors and toxin producing genes.
Tárgyszavak:	Orvostudományok Egészségtudományok előadáskivonat könyvrészlet AMR antibiotic resistant bacteria Common carp MALDI-TOF MS
Megjelenés:	15th Egerton University Biennial International Conference : Programme and book of abstracts / ed. M.K. Charimbu. - p. 42
További szerzők:	Bőkényné Tóth Renáta Kardos Gábor (1974-) (absztraktok) Pál Károly (1978-) (mikrobiológus)
Internet cím:	Intézményi repozitóriumban (DEA) tárolt változat
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