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001-es BibID:	BIBFORM117605
035-os BibID:	(cikkazonosító)670380 (WoS)000660647600001 (Scopus)85107691476
Első szerző:	Khalfalla Maha (PhD hallgató / nutrition science)
Cím:	Isolation, Extraction, Purification, and Molecular Characterization for Thermostable α -Amylase from Locally Isolated Bacillus Species in Sudan / Rakaz, Maha A.; Hussien, Mohammed O.; Ibrahim, Hanan M.
Dátum:	2021
ISSN:	2090-2247 2090-2255
Megjegyzések:	The aim of this study was to isolate some soil bacteria strain that produced ?-amylase and subsequent extraction and purification.One hundred soil samples were collected from different geographical areas in Khartoum State such as north Omdurman, TotiIsland, and Soba. Samples were analyzed for starch hydrolyzing bacteria. Among several bacteria isolated, Bacillus cereus andBacillus licheniformis were identified as active ?-amylase producers. Both bacteria showed a large zone of clearance of 20 mm whengrown on starch-agar plates. +e identity was conducted using biochemical characterization and confirmed by sequencing their16S-rDNA. +e constitutive nature of amylase was proved by amplification of the amylase gene from the genome ofB. licheniformis. +e ?-amylase activity from the spent medium of B. cereus and B. licheniformis was optimized at pH 8.0 andtemperature of 45?C and 65?C, respectively. +e ?-amylase produced by both bacteria is alkalophilic and thermophilic. +eexperiments confirmed that B. licheniformis can be a good source of amylase for industrial applications in Sudan
Tárgyszavak:	Agrártudományok Élelmiszertudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk
Megjelenés:	Biochemistry Research International. - 2021 (2021), p. 1-8. -
További szerzők:	Hussien, Mohammed O. Ibrahim, Hanan M.
Pályázati támogatás:	SHE Other
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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