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001-es BibID:	BIBFORM018166
Első szerző:	Orosz László (Szeged)
Cím:	Involvement of p63 in the herpes simplex virus-1-induced demise of corneal cells / Orosz László, Gallyas Éva, Kemény Lajos, Mándi Yvette, Facskó Andrea, Megyeri Klára
Dátum:	2010
ISSN:	1423-0127
Megjegyzések:	The transcription factor p63 plays a pivotal role in the development and maintenance of epithelial tissues, including the ocular surface. In an effort to gain insight into the pathogenesis of keratitis caused by HSV-1, we determined the expression patterns of the p63 and Bax proteins in the Staatens Seruminstitute Rabbit Cornea cell line (SIRC). Methods SIRC cells were infected with HSV-1 at various multiplicities and maintained for different periods of time. Virus replication was measured by indirect immunofluorescence assay and Western blot analysis. Cell viability was determined by MTT assay. The apoptotic response of the infected cells was quantified by ELISA detecting the enrichment of nucleosomes in the cytoplasm. Western blot analysis was used to determine the levels of p63 and Bax proteins. Results Indirect immunofluorescence assays and Western blot analyses demonstrated the presence of HSV-1 glycoprotein D (gD) in the infected SIRC cell line, and the pattern of gD expression was consistent with efficient viral replication. The results of MTT and ELISA assays showed that HSV-1 elicited a strong cytopathic effect, and apoptosis played an important role in the demise of the infected cells. Mock-infected SIRC cells displayed the constitutive expression of ?Np63?. The expressions of the Bax-? and TAp63? isoforms were considerably increased, whereas the level of ?Np63? was decreased in the HSV-1-infected SIRC cells. Experiments involving the use of acyclovir showed that viral DNA replication was necessary for the accumulation of TAp63?. Conclusion These data suggest that a direct, virus-mediated cytopathic effect may play an important role in the pathogenic mechanism of herpetic keratitis. By disturbing the delicate balance between the pro-survival ?N and the pro-apoptotic TA isoforms, HSV-1 may cause profound alterations in the viability of the ocular cells and in the tissue homeostasis of the ocular surface.
Tárgyszavak:	Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:	Journal of Biomedical Science. - 17 : 1 (2010), p. 47. -
További szerzők:	Gallyas Éva Kemény Lajos Mándi Yvette Facskó Andrea (1953-) (szemész) Megyeri Klára
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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001-es BibID:	BIBFORM062340
Első szerző:	Petrovski, Goran (orvos)
Cím:	Herpes simplex virus types 1 and 2 modulate autophagy in SIRC corneal cells / Goran Petrovski, Kata Pásztor, László Orosz, Réka Albert, Edina Mencel, Morten C. Moe, Kai Kaarniranta, Andrea Facskó, Klára Megyeri
Dátum:	2014
ISSN:	0250-5991
Megjegyzések:	Autophagy and apoptosis function as important early cellular defense mechanisms in infections and other diseases. The outcome of an infection is determined by a complex interplay between the pathogenic microorganism and these intracellular pathways. To better understand the cytopathogenicity of Herpes simplex virus types 1 and 2 (HSV-1 and - 2), we studied the effect of these viruses on the autophagic and apoptotic processes in the SIRC corneal cell line. Infection with the KOS strain of HSV-1 and a wild-type strain of HSV-2 enhanced autophagosome formation, triggered cytoplasmic acidification, increased LC3B lipidation and elevated the ratio of apoptotic cells. The autophagy inhibitor bafilomycin A1 triggered a significant increase in the apoptotic responses of HSV-1 and HSV-2-infected cells. Thus, both HSV types affect autophagy and apoptosis in a coordinated fashion, and autophagy plays cytoprotective role in HSV-infected cells via antagonizing apoptosis. Together these data implicate autophagy in the pathogenic mechanism of herpetic keratitis.
Tárgyszavak:	Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban Apoptosis autophagy corneal cells Herpes simplex virus keratitis
Megjelenés:	Journal Of Biosciences. - 39 : 4 (2014), p. 683-692. -
További szerzők:	Pásztor Kata Orosz László (Szeged) Albert Réka (1986-) Mencel Edina Moe, Morten C. Kaarniranta, Kai (1972-) (szemész szakorvos) Facskó Andrea (1953-) (szemész) Megyeri Klára
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