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001-es BibID:	BIBFORM059505
Első szerző:	Froen, Rebecca C.
Cím:	Does the Adult Human Ciliary Body Epithelium Contain "True" Retinal Stem Cells? / Froen Rebecca, Johnsen Erik O., Nicolaissen Bjorn, Facskó Andrea, Petrovski Goran, Moe Morten C.
Dátum:	2013
ISSN:	2314-6133 2314-6141
Megjegyzések:	Recent reports of retinal stem cells being present in several locations of the adult eye have sparked great hopes that they may be used to treat the millions of people worldwide who suffer from blindness as a result of retinal disease or injury. A population of proliferative cells derived from the ciliary body epithelium (CE) has been considered one of the prime stem cell candidates, and as such they have received much attention in recent years. However, the true nature of these cells in the adult human eye has still not been fully elucidated, and the stem cell claim has become increasingly controversial in light of new and conflicting reports. In this paper, we will try to answer the question of whether the available evidence is strong enough for the research community to conclude that the adult human CE indeed harbors stem cells.
Tárgyszavak:	Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban NEURAL PROGENITOR CELLS MAMMALIAN EYE HUMAN BRAIN PROLIFERATIVE VITREORETINOPATHY STEM/PROGENITOR CELLS NEUROSPHERE ASSAY DIFFERENTIATION SYSTEM PHOTORECEPTORS IDENTIFICATION
Megjelenés:	Biomed Research International. - 2013 : Article ID 531579 (2013), p. 1-7. -
További szerzők:	Johnsen, Erik O. Nicolaissen, Bjorn Facskó Andrea (1953-) (szemész) Petrovski, Goran (1975-) (orvos) Moe, Morten C.
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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001-es BibID:	BIBFORM037061
Első szerző:	Froen, Rebecca C.
Cím:	Pigment epithelial cells isolated from human peripheral iridectomies have limited properties of retinal stem cells / Froen R. C., Johnsen E. O., Petrovski G., Berényi E., Facskó A., Berta A., Nicolaissen B., Moe M. C.
Dátum:	2011
ISSN:	1755-375X
Megjegyzések:	PURPOSE: The identification of cells with properties of retinal progenitor cells (RPCs) in the adult human ciliary margin (CM) prompted a number of studies of their proliferative and differentiation potential. One of the remaining challenges is to find a feasible method of isolating RPCs from the patient's eye. In the human CM, only the iris pigment epithelium (IPE) is easily obtained by a minimally invasive procedure. In the light of recent studies questioning the existence of RPCs in the adult mammalian CM, we wanted to assess the potential of the adult human IPE as source of RPCs. METHODS: The IPE were isolated from peripheral iridectomies during glaucoma surgery, and IPE and ciliary body (CB) epithelium were also isolated from post-mortem tissue. Cells were cultivated in sphere-promoting conditions or as monolayers. Whole-tissue samples, undifferentiated and differentiated cells were studied by immunocytochemistry, RT-PCR and transmission electron microscopy. RESULTS: The adult human IPE, like the CB, expressed markers of RPCs such as Pax6, Sox2 and Nestin in vivo. Both sphere-promoting and monolayer cultures preserved this phenotype. However, both IPE/CB cultures expressed markers of differentiated epithelial cells such as Claudin, microphtalmia-associated transcription factor (MITF) and Cytokeratin-19. Ultrastructurally, IPE spheres displayed epithelial-like junctions and contained mature melanosomes. After induced differentiation, IPE-derived cells showed only partial neuronal differentiation expressing beta-III-tubulin, Map-2 and Rhodopsin, whereas no mature glial markers were found. CONCLUSION: Proliferative cells with some properties of RPCs can be isolated from the adult human IPE by peripheral iridectomies. Yet, many cells retain properties of differentiated epithelial cells and lack central properties of somatic stem cells.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:	Acta Ophthalmologica. - 89 : 8 (2011), p. 635-644. -
További szerzők:	Johnsen, Erik O. Petrovski, Goran (1975-) (orvos) Berényi Erika Facskó Andrea (1953-) (szemész) Berta András (1955-) (szemész, gyermekszemész) Nicolaissen, Bjorn Moe, Morten C.
Internet cím:	Intézményi repozitóriumban (DEA) tárolt változat DOI
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