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001-es BibID:bibEBI00019288
Első szerző:Kemény-Beke Ádám (szemész)
Cím:Antiproliferative effect of 4-thiouridylate on OCM-1 uveal melanoma cells / Kemény-Beke A., Berényi E., Facskó A., Damjanovich J., Horváth A., Bodnár A., Berta A., Aradi J.
Dátum:2006
Megjegyzések:Brachytherapy is a well-established, effective treatment for uveal melanoma with a failure rate of 15%. The fatal consequence of unsuccessful treatments offers reason for improvement of the method. The authors propose using an apoptosis inducing agent locally, concomitantly with the well-established therapy, to sensitize the tumor cells. The authors propose a new nontoxic moderately active apoptosis inducing agent, 4-thio-uridylate (s4UMP), for this purpose. METHODS: OCM-1 uveal melanoma cells were treated with various concentrations of s4UMP and its effect was monitored by measuring the cell viability (MTT assay). The following apoptosis detecting methods were performed to reveal the mechanism of decreased cell viability: light microscopy, DNA fragmentation assay, determination of caspase 9 activity, and FACS analysis. RESULTS: The viability of uveal melanoma cells was decreased by 32%, 40%, and 9% after 24, 48, and 72 hours of treatment with 10 microg/mL (30 microM) s4UMP. The effect was not dose dependent; it rather followed a saturation-type inhibition and the cells at lower drug concentration recovered after 72 hours. Characteristic apoptotic cell morphology and DNA fragmentation was detected in treated cells. The caspase-9 was activated upon treatment showing maximal activity at 48 hours suggesting the induction of apoptosis. The annexin binding activity further verified the apoptogenic activity of s4UMP. CONCLUSIONS: Uveal melanoma, more than other solid tumors, is resistant to most of the chemotherapeutic protocols as indicated by the high mortality rate of metastatic disease. The authors showed that s4UMP, a naturally occurring nucleotide, could induce apoptosis in uveal melanoma cells, suggesting a potential supplementary therapeutic application of the compound.
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:European Journal of Ophthalmology. - 16 : 5 (2006), p. 680-685. -
További szerzők:Berényi Erika Facskó Andrea (1953-) (szemész) Damjanovich Judit (1963-) (szemész) Horváth A. Dóczy-Bodnár Andrea (1970-) (biofizikus) Berta András (1955-) (szemész, gyermekszemész) Aradi János (1942-) (biokémikus, vegyész)
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001-es BibID:BIBFORM078207
Első szerző:Surányi Éva (szemész)
Cím:Does Beta-ray Emitting Therapy of Ciliary Body Tumors Decrease Central Corneal Endothelial Cell Density? / Suranyi Eva, Berta Andras, Modis Laszlo, Szalai Eszter, Damjanovich Judit
Dátum:2013
ISSN:1120-6721
Megjegyzések:PURPOSE: To evaluate the effect of plaque radiation therapy using beta-radioactive isotope for anterior segment tumors on the density and morphology of endothelial cells. ? METHODS: Endothelial cell density (ECD) and morphometry in 15 eyes of 15 patients with ciliary body tumor was examined prior to and 6 months after radiation therapy. After irradiation, central ECD values were also compared with peripheral (2.0 mm from limbus) ECD values measured around the plaque. The ECD, average cell area, coefficient of variation of cell area, and pachymetry measurements were conducted with contact specular microscopy.? RESULTS: The mean corrected ECD values prior to irradiation were 2147 ? 128 cells/mm2 and 2050 ? 108 cells/mm2 after the radiation therapy. After irradiation, the mean peripheral ECD values were 2056 ? 101 cells/mm2. A significant decrease in ECD values was observed after radiation (p = 0.007). Peripheral ECD values measured around the plaque showed no significant difference (p = 0.86) as compared to central ECD values.? CONCLUSIONS: According to our measurements, plaque therapy for tumors in the anterior segment decreases ECD significantly, but not highly, even in case of plaques containing beta-radiation isotope, and the plaques are not placed direct on the cornea surface. The decreased ECD causes no changes in corneal thickness or transparency, but it may have an influence on a subsequent cataract surgery, which generates further endothelial loss.
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Beta-radiation
Ciliary body tumor
Endothelial cell
Plaque therapy
Megjelenés:European Journal Of Ophthalmology. - 23 : 5 (2013), p. 623-628. -
További szerzők:Berta András (1955-) (szemész, gyermekszemész) Módis László (1964-) (szemész szakorvos, kontaktológus) Szalai Eszter (1984-) (orvos) Damjanovich Judit (1963-) (szemész)
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