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1.

001-es BibID:BIBFORM043609
Első szerző:Albert Réka
Cím:Cultivation and characterization of cornea limbal epithelial stem cells on lens capsule in animal material-free medium / Réka Albert, Zoltán Veréb, Krisztián Csomós, Morten C. Moe, Erik O. Johnsen, Ole Kristoffer Olstad, Bjørn Nicolaissen, Éva Rajnavölgyi, László Fésüs, András Berta, Goran Petrovski
Dátum:2012
ISSN:1932-6203
Megjegyzések:A simple, reproducible, animal-material free method for cultivating and characterizing cornea limbal epithelial stem cells (LESCs) on human lens capsule (LC) was developed for future clinical transplantation. The limbal tissue explants (2 ? 2 ? 0.25 mm) were harvested from 77 cadavers and expanded ex vivo on either cell culture plates or LC in medium containing human serum as the only growth supplement. Cell outgrowth at the edge of the explants was observed within 24 hours of cultivation and achieved viable outgrowth (>97% viability as measured by MTT assay and flow cytometry) within two weeks. The outgrowing cells were examined by genome-wide microarray including markers of stemness (p63?, ABCG2, CK19, Vimentin and Integrin ?9), proliferation (Ki-67), limbal epithelial cells (CK 8/18 and 14) and differentiated cornea epithelial cells (CK 3 and 12). Immunostaining revealed the non-hematopoietic, -endothelial and -mesenchymal stem cell phenotype of the LESCs and the localization of specific markers in situ. Cell adhesion molecules, integrins and lectin-based surface carbohydrate profiling showed a specific pattern on these cells, while colony-formation assay confirmed their clonal potency. The LESCs expressed a specific surface marker fingerprint (CD117/c-kit, CXCR4, CD144/VE-Cadherin, CD146/MCAM, CD166/ALCAM, and surface carbohydrates: WGA, ConA, RCA, PNA and AIL) which can be used for better localization of the limbal stem cell niche. In summary, we report a novel method combining the use of a medium with human serum as the only growth supplement with LC for cultivating, characterizing and expanding cornea LESCs from cadavers or alternatively from autologous donors for possible treatment of LESC deficiency.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Limbal epithelial stem cell
egyetemen (Magyarországon) készült közlemény
Megjelenés:PLoS One. - 7 : 10 (2012), p. e47187. -
További szerzők:Veréb Zoltán (1980-) (immunológus, mikrobiológus, molekuláris biológus) Csomós Krisztián (1981-) (molekuláris biológus) Moe, Morten C. Johnsen, Erik O. Olstad, Ole Kristoffer Nicolaissen, Bjorn Rajnavölgyi Éva (1950-) (immunológus) Fésüs László (1947-) (orvos biokémikus) Berta András (1955-) (szemész, gyermekszemész) Petrovski, Goran (1975-) (orvos)
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2.

001-es BibID:BIBFORM013626
Első szerző:Fodor Mariann (szemész)
Cím:Spectroscopic study of explanted opacified hydrophilic acrylic intraocular lenses / Fodor M., Petrovski G., Moe M. C., Bíró Z., Németh G., Dinya Z., Tornai I., Ujvári T., Berta A., Facskó A.
Dátum:2011
ISSN:1755-3768
Megjegyzések:To report a detailed spectroscopic analysis of explanted hydrophilic acrylic intraocular lenses (IOLs) that were removed because of postoperative opacification of the lens optic. Methods: Thirteen Hydroview H60M (Bausch & Lomb Surgical) IOLs were explanted from 13 different patients on average 56 months after phacoemulsification and IOL implantation. All patients had decreased visual acuity because of a fine granularity of the optical surface of the IOLs. The surface was investigated by gross, microscopic, histochemical and scanning electron microscopic analysis, and the elemental composition of the opacified IOLs was determined by X-ray fluorescence spectroscopy (XRF). The spectrograms were compared to three different originally packed and never-implanted hydrophilic acrylic IOLs. Results: Light and scanning electron microscopy of the optical surface of explanted IOLs revealed multiple fine granular deposits varying in size and shape that were positive for alizarin red. XRF confirmed that the explanted IOLs contained not only the previously reported calcium and phosphorous (calcium apatite), chlorine, silicone, sodium, aluminum and magnesium but also iron, sulfur, potassium as well as lesser amounts of iodine, zinc, strontium and yttrium. Conclusion: This is the first spectroscopic analysis determining the content of more than 10 elements of explanted and originally packed never-implanted hydrophilic acrylic IOLs. The possible origin of the different elements obtained from the spectrograms and their implications are discussed.
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Megjelenés:Acta Ophthalmologica. - 89 : 2 (2011), p. e161-e166. -
További szerzők:Petrovski, Goran (1975-) (orvos) Moe, Morten C. Biró Zsolt (szemész) Németh Gábor (1975-) (szemész) Dinya Zoltán (1942-) (vegyész) Tornai Ildikó Ujvári Tamás Berta András (1955-) (szemész, gyermekszemész) Facskó Andrea (1953-) (szemész)
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3.

001-es BibID:BIBFORM037061
Első szerző:Froen, Rebecca C.
Cím:Pigment epithelial cells isolated from human peripheral iridectomies have limited properties of retinal stem cells / Froen R. C., Johnsen E. O., Petrovski G., Berényi E., Facskó A., Berta A., Nicolaissen B., Moe M. C.
Dátum:2011
ISSN:1755-375X
Megjegyzések:PURPOSE: The identification of cells with properties of retinal progenitor cells (RPCs) in the adult human ciliary margin (CM) prompted a number of studies of their proliferative and differentiation potential. One of the remaining challenges is to find a feasible method of isolating RPCs from the patient's eye. In the human CM, only the iris pigment epithelium (IPE) is easily obtained by a minimally invasive procedure. In the light of recent studies questioning the existence of RPCs in the adult mammalian CM, we wanted to assess the potential of the adult human IPE as source of RPCs. METHODS: The IPE were isolated from peripheral iridectomies during glaucoma surgery, and IPE and ciliary body (CB) epithelium were also isolated from post-mortem tissue. Cells were cultivated in sphere-promoting conditions or as monolayers. Whole-tissue samples, undifferentiated and differentiated cells were studied by immunocytochemistry, RT-PCR and transmission electron microscopy. RESULTS: The adult human IPE, like the CB, expressed markers of RPCs such as Pax6, Sox2 and Nestin in vivo. Both sphere-promoting and monolayer cultures preserved this phenotype. However, both IPE/CB cultures expressed markers of differentiated epithelial cells such as Claudin, microphtalmia-associated transcription factor (MITF) and Cytokeratin-19. Ultrastructurally, IPE spheres displayed epithelial-like junctions and contained mature melanosomes. After induced differentiation, IPE-derived cells showed only partial neuronal differentiation expressing beta-III-tubulin, Map-2 and Rhodopsin, whereas no mature glial markers were found. CONCLUSION: Proliferative cells with some properties of RPCs can be isolated from the adult human IPE by peripheral iridectomies. Yet, many cells retain properties of differentiated epithelial cells and lack central properties of somatic stem cells.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:Acta Ophthalmologica. - 89 : 8 (2011), p. 635-644. -
További szerzők:Johnsen, Erik O. Petrovski, Goran (1975-) (orvos) Berényi Erika Facskó Andrea (1953-) (szemész) Berta András (1955-) (szemész, gyermekszemész) Nicolaissen, Bjorn Moe, Morten C.
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4.

001-es BibID:BIBFORM037065
Első szerző:Johnsen, Erik O.
Cím:Activation of neural progenitor cells in human eyes with proliferative vitreoretinopathy / Johnsen E. O., Froen R. C., Albert R., Omdal B. K., Sarang Z., Berta A., Nicolaissen B., Petrovski G., Moe M. C.
Dátum:2012
ISSN:0014-4835
Megjegyzések:In addition to the ability for self-renewal and functional differentiation, neural stem/progenitor cells (NSCs) can respond to CNS injuries by targeted migration. In lower vertebrates, retinal injury is known to activate NSCs in the ciliary marginal zone (CMZ). Cells expressing markers of NSCs are also present in the ciliary body epithelium (CE) and in Müller glia in the peripheral retina (PR) of the adult human eye. However, these cells seem to be quiescent in the adult human eye and recent reports have shown that CE cells have limited properties of NSCs. In order to further clarify whether NSCs exist in the adult human eye, we tested whether NSC-like cells could be activated in eyes with proliferative vitreoretinopathy (PVR). The PR and CE were studied for NSC-associated markers in human enucleated control eyes and eyes with confirmed PVR, as well as in a mouse model of PVR. Furthermore, cells isolated from vitreous samples obtained during vitrectomies for retinal detachment were directly fixed or cultured in a stem cell-promoting medium and compared to cells cultured from the post-mortem retina and CE. In situ characterization of the normal eyes revealed robust expression of markers present in NSCs (Nestin, Sox2, Pax6) only around peripheral cysts of the proximal pars plana region and the PR, the latter population also staining for the glial marker GFAP. Although there were higher numbers of dividing cells in the CE of PVR eyes than in controls, we did not detect NSC-associated markers in the CE except around the proximal pars plana cysts. In the mice PVR eyes, Nestin activation was also found in the CE. In human PVR eyes, proliferation of both non-glial and glial cells co-staining NSC-associated markers was evident around the ora serrata region. Spheres formed in 7/10 vitreous samples from patients with PVR compared to 2/15 samples from patients with no known PVR, and expressed glial - and NSC-associated markers both after direct fixation and repetitive passages. In conclusion, the adult human eye may harbor two different populations of neuroepithelial stem/progenitor cells; a non-glial population located in the proximal pars plana around peripheral cysts in addition to a population with Müller glia characteristics. Yet, we only found that the glial population was able to respond to retinal injury by targeted migration into the vitreous.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:Experimental Eye Research. - 98 (2012), p. 28-36. -
További szerzők:Froen, Rebecca C. Albert Réka (1986-) Omdal, Bente K. Sarang Zsolt (1976-) (mikrobiológus) Berta András (1955-) (szemész, gyermekszemész) Nicolaissen, Bjorn Petrovski, Goran (1975-) (orvos) Moe, Morten C.
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5.

001-es BibID:BIBFORM042067
035-os BibID:PMID:22112056
Első szerző:Kinnunen, Kati
Cím:Molecular mechanisms of retinal pigment epithelium damage and development of age-related macular degeneration / Kati Kinnunen, Goran Petrovski, Morten C. Moe, András Berta, Kai Kaarniranta
Dátum:2012
ISSN:1755-375X
Megjegyzések:Age-related macular degeneration (AMD) is attributed to a complex interaction of genetic and environmental factors. It is characterized by degeneration involving the retinal photoreceptors, retinal pigment epithelium (RPE) and Bruch's membrane, as well as alterations in choroidal capillaries. AMD pathogenesis is strongly associated with chronic oxidative stress and inflammation that ultimately lead to protein damage, aggregation and degeneration of RPE. Specific degenerative findings for AMD are accumulation of intracellular lysosomal lipofuscin and extracellular drusens. In this review, we discuss thoroughly RPE-derived mechanisms in AMD pathology. © 2011 The Authors.
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
külföldön készült közlemény
Megjelenés:Acta Ophthalmologica. - 90 : 4 (2012), p. 299-309. -
További szerzők:Petrovski, Goran (1975-) (orvos) Moe, Morten C. Berta András (1955-) (szemész, gyermekszemész) Kaarniranta, Kai (1972-) (szemész szakorvos)
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6.

001-es BibID:BIBFORM049105
Első szerző:Petrovski, Goran (orvos)
Cím:Autophagy in the eye : a double-edged sword / Goran Petrovski, Réka Albert, Kai Kaarniranta, Morten C. Moe, László Fésüs, András Berta, Dipak K. Das
Dátum:2012
Megjegyzések:Autophagy, a cytoplasmic recycling process active from early development throughout lifetime and aging has turned from largely a morphological phenomenon to a well described molecular mechanism in health and disease. The eye as an organ in which an interplay of three germ layers is responsible for achieving complex visual response, is the place where battles for cellular life or death takes place continuously ? an organ in which the highest dead cell turnover occurs in the human body. The role of autophagy in the different segments of the eye remains not so well understood or described, making it difficult to classify autophagy as a protective or disease causing process. This chaptersummarizes the current knowledge about the role of autophagy in eye health and disease as well as the present drug targets applying autophagy as a protective mechanism against ophthalmic neurodegeneration and aging.
ISBN:978 1 61942 266 7
Tárgyszavak:Orvostudományok Elméleti orvostudományok könyvfejezet
Autophagy
double-edged sword
Megjelenés:Autophagy: Principles, Regulation and Roles in Disease / szerk. Nikolai Gorbunov. - p. 157-180. -
További szerzők:Albert Réka (1986-) Kaarniranta, Kai (1972-) (szemész szakorvos) Moe, Morten C. Fésüs László (1947-) (orvos biokémikus) Berta András (1955-) (szemész, gyermekszemész) Das, Dipak Kumar
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7.

001-es BibID:BIBFORM028036
Első szerző:Petrovski, Goran (orvos)
Cím:Clearance of dying ARPE-19 cells by professional and nonprofessional phagocytes in vitro- implications for age-related macular degeneration (AMD) / Petrovski Goran, Berényi Erika, Moe Morten C., Vajas Attila, Fésüs László, Berta András, Facskó Andrea
Dátum:2011
ISSN:1755-375X
Megjegyzések:Failure of retinal pigment epithelial (RPE) cells and macrophages to engulf different dying cells in the retina may result in accumulation of debris and development of age-related macular degeneration (AMD). The dynamics and influence of different treatments on this clearance process can be studied in vitro using human ARPE-19 cells and macrophages as phagocytes modelling dry and wet type of AMD, respectively. METHODS: Death through extracellular matrix detachment using polyHEMA-coated surfaces (anoikis) and UV irradiation (apoptosis) was induced in ARPE-19 cells. Two-coloured phagocytic assays were performed to quantify the amount of dying cells phagocytes engulfed (flow cytometry) and for visualization (fluorescent and scanning electron microscopy). The effect of phosphatidylserine inhibition with recombinant annexin-V and glucocorticoid (triamcinolone) treatment on the phagocytic process was tested. RESULTS: The clearance of anoikic and apoptotic cells by nondying ARPE-19 cells over 8 hr of co-incubation increased over time (at 8 hr, over 53% and 35% of the phagocytes contained engulfed dying cells, respectively). The human macrophages engulfed the anoikic and apoptotic ARPE-19 cells with seven and four times lower capacity, respectively. Phosphatidylserine appearance on the dying cells did not affect, but triamcinolone treatment enhanced the phagocytosis of the dying cells by macrophages. CONCLUSIONS: ARPE-19 cells are more efficient in clearing anoikic than UV-induced apoptotic cells. Macrophages are less efficient in the clearance process than ARPE-19 cells. The present model can be used for studying both dry and wet type of AMD in vitro and for testing different pharmacological aspects affecting this disease.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:Acta Ophthalmologica. - 89 : 1 (2011), p. 30-34. -
További szerzők:Berényi Erika Moe, Morten C. Vajas Attila (1973-) (szemész) Fésüs László (1947-) (orvos biokémikus) Berta András (1955-) (szemész, gyermekszemész) Facskó Andrea (1953-) (szemész)
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