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1.

001-es BibID:bibEB00010212
035-os BibID:PMID:7694470
Első szerző:Juhász István (bőrgyógyász, bőrsebész, kozmetológus, klinikai onkológus)
Cím:Regulation of extracellular matrix proteins and integrin cell substratum adhesion receptors on epithelium during cutaneous human wound healing in vivo / Istvan Juhasz, George F. Murphy, Horng-Chin Yan, Meenhard Herlyn, Steven M. Albelda
Dátum:1993
Megjegyzések:Although changes in extracellular matrix proteins during wound healing have been well documented, little is known about the regulation of corresponding extracellular matrix adhesion receptors (integrins). To study this process in a human in vivo model, full thickness human skin grafts were transplanted onto severe combined immunodeficient mice and deep excisional wounds involving both the epidermal and dermal layers were then made. The changes in the expression of cell matrix proteins and epithelial integrins over time were analyzed with specific antibodies using immunohistochemistry. Wounding was associated with alterations in extracellular matrix proteins, namely, loss of laminin and type IV collagen in the region of the wound and expression of tenascin and fibronectin. Changes were also noted in the integrins on the migrating keratinocytes. There was marked up-regulation of the alpha v subunit and de novo expression of the fibronectin receptor (alpha 5 beta 1) during the stage of active migration (days 1 to 3 after wounding). In the later stages of wound healing, after epithelial integrity had been established, redistribution of the alpha 2, alpha 3, alpha 6, and beta 4 collagen/laminin-binding integrin subunits to suprabasal epidermal layers was noted. Thus, during cutaneous wound healing, keratinocytes up-regulate fibronectin/fibrinogen-binding integrins and redistribute collagen/laminin-binding integrins. This study demonstrates that the human skin/severe combined immunodeficient chimera provides a useful model to study events during human wound repair.
Tárgyszavak:idegen nyelvű folyóiratközlemény külföldi lapban
külföldön készült közlemény
Megjelenés:American Journal of Pathology. - 143 : 5 (1993), p. 1458-1469. -
További szerzők:Murphy, George F. Yan, Horng-Chin Herlyn, Meenhard Albelda, Steven M.
Internet cím:elektronikus változat
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2.

001-es BibID:bibEB00010211
035-os BibID:PMCID:PMC288423
Első szerző:Juhász István (bőrgyógyász, bőrsebész, kozmetológus, klinikai onkológus)
Cím:Development of pemphigus vulgaris-like lesions in severe combined immunodeficiency disease mice reconstituted with lymphocytes from patients / Istvan Juhasz, Gerald S. Lazarus, George F. Murphy, Ie-Ming Shih, Meenhard Herlyn
Dátum:1993
Megjegyzések:Pemphigus vulgaris is an autoimmune blistering disease that is induced by binding of antibodies to a 130/85-kD protein complex on epidermal keratinocytes. An in vivo experimental model of this disease was developed by reconstituting severe combined immunodeficiency (SCID) mice with 1-10 x 10(7) PBL from patients with naturally occurring pemphigus vulgaris. Of 49 reconstituted mice, 34 (69%) produced human IgG levels of > 0.1 mg/ml. Circulating anti-pemphigus antibodies were found in 20 of the 34 successfully reconstituted mice; 44% of these animals had deposits of human IgG in their own skin after it was traumatized by either heat or cold. Spontaneous pemphigus vulgaris-like blisters associated with human IgG deposits were rarely found in mouse skin. By contrast, allogeneic human skin grafted to 10 to 12 mice before reconstitution with patients' PBL developed pemphigus vulgaris-like lesions containing human IgG deposits. These results demonstrate that SCID mice can serve as a model of an antibody-mediated human autoimmune skin disease.
Tárgyszavak:idegen nyelvű folyóiratközlemény külföldi lapban
külföldön készült közlemény
Megjelenés:Journal of Clinical Investigation. - 92 : 5 (1993), p. 2401-2407. -
További szerzők:Lazarus, Gerald S. Murphy, George F. Herlyn, Meenhard Shih, Ie-Ming
Internet cím:DOI
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3.

001-es BibID:bibEB00010210
035-os BibID:PMID:8342600
Első szerző:Juhász István (bőrgyógyász, bőrsebész, kozmetológus, klinikai onkológus)
Cím:Growth and invasion of human melanomas in human skin grafted to immunodeficient mice / Istvan Juhasz, Steven M. Albelda, David E. Elder, George F. Murphy, Koji Adachi, Dorothee Herlyn, Istvan T. Valyi-Nagy, Meenhard Herlyn
Dátum:1993
Megjegyzések:An orthotopic model of human melanoma was developed in which malignant cells were injected into human skin grafted to nude and SCID mice. Melanoma cells proliferated and invaded the human skin grafts with characteristic patterns. Three of six melanomas grew as multiple nodules and infiltered the grafts without major architectural changes in the dermis, whereas the others invaded the dermis along collagen fibers with prominent endothelial vessels. By contrast, melanoma cells inoculated into mouse skin grew as diffusely expanding nodules that did not invade the murine dermis. In human skin grafts, human melanoma cells were angiogenic for human blood vessels, and murine vessels were only found at the periphery of grafts. Tumor cells invaded the human vessels, and four out of seven cell lines metastasized to lungs, suggesting that this model is useful to determine in vivo the interactions between normal and malignant human cells.
Tárgyszavak:idegen nyelvű folyóiratközlemény külföldi lapban
külföldön készült közlemény
Megjelenés:American Journal of Pathology. - 143 : 2 (1993), p. 528-537. -
További szerzők:Albelda, Steven M. Elder, David E. Murphy, George F. Adachi, Koji Herlyn, Dorothee Vályi-Nagy István Herlyn, Meenhard
Internet cím:elektronikus változat
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4.

001-es BibID:bibEBI14267
Első szerző:Pilewski, J. M.
Cím:Modulation of adhesion molecules by cytokines in vivo using human/severe combined immunodeficient (SCID) mouse chimeras / Pilewski, J. M., Yan, H-C., Juhász I., Christofidou-Solomidou, M., Williams, J., Murphy, G. F., Albelda, S. M.
Dátum:1995
Megjegyzések:Endothelial cell-leukocyte interactions involve multiple cell adhesion molecules acting in a programmed and sequential manner to create a leukocyte-endothelial cell adhesion cascade. To understand this process fully, in vivo models are needed. To accomplish this, we have transplanted pieces of normal human tissues onto immunodeficient mice to create chimeric animals. In one model, human skin is grafted and closely resembles normal skin histologically. The grafts retain their human vasculature and show low baseline expression of E-selectin, vascular cell adhesion molecule-1, and intercellular cell adhesion molecule-1. After intradermal injection of human cytokines, these cell adhesion molecules are markedly upregulated and an active inflammatory reaction ensues, with migration of murine leukocytes. Intravenous injection of an anti-human E-selectin antibody completely inhibits leukocyte accumulation induced by tumor necrosis factor-alpha but only partially inhibits leukotriene B4-induced inflammation. In a second model, human bronchus was successfully transplanted heterotopically into severe combined immunodeficient mice. Injection of tumor necrosis factor induced upregulation of E-selectin, intercellular cell adhesion molecule-1, and vascular cell adhesion molecule-1 in the submucosal microvessels, with slightly different kinetics than in the skin. In conclusion, human-severe combined immunodeficient chimeric mice represent a useful model system to study the regulation and function of human cell adhesion molecules in an in vivo setting.
Tárgyszavak:idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:Journal of clinical immunology. - 15 : Suppl.6 (1995), p. 122S-129S. -
További szerzők:Yan, Horng-Chin Juhász István (1956-) (bőrgyógyász, bőrsebész, kozmetológus, klinikai onkológus) Christofidou-Solomidou, M. Williams, J. Murphy, George F. Albelda, Steven M.
Internet cím:DOI
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5.

001-es BibID:bibEBI12849
Első szerző:Yan, Homg-Chin
Cím:Human/severe combined immunodeficient mouse chimeras : an experimental in vivo model system to study the regulation of human endothelial cell-leukocyte adhesion molecules / Homg-Chin Yan, Istvan Juhasz, Joseph Pilewski, George F. Murphy, Meenhard Herlyn, Steven M. Albelda
Dátum:1993
ISSN:0021-9738 1558-8238
Megjegyzések:The ability of circulating white blood cells to enter inflamed tissues is mediated by specific cell adhesion molecules thought to be expressed in a programmed and sequential manner to form an "adhesion cascade." Because of the complexity of this process, it is becoming increasingly important to develop in vivo models. Two major problems have limited the utility of current animal models. The first is the inability of many of the antibodies developed against cell adhesion molecules in human cell culture models to cross-react in animals. The second is the uncertainty in extrapolating animal (particularly rodent) findings to humans. To circumvent these problems, full thickness human skin grafts were transplanted onto immunodeficient (severe combined immunodeficient) mice. After 4-6 wk, the transplanted skin grafts closely resembled normal skin histologically and maintained their human vasculature as determined by immunohistochemical staining with human-specific endothelial cell markers. Intradermal injection of tumor necrosis factor-alpha resulted in the reversible upregulation of the leukocyte-endothelial adhesion molecules E-selectin, vascular cell adhesion molecule-1, and intercellular adhesion molecule-1, and in an active inflammatory reaction with migration of murine leukocytes into cytokine-injected areas. These results indicate that the severe combined immunodeficient mouse/human skin transplant model provides a useful in vivo system in which to study human endothelium during the process of inflammation.
Tárgyszavak:idegen nyelvű folyóiratközlemény külföldi lapban
külföldön készült közlemény
Megjelenés:Journal of Clinical Investigation. - 91 : 3 (1993), p. 986-996. -
További szerzők:Juhász István (1956-) (bőrgyógyász, bőrsebész, kozmetológus, klinikai onkológus) Pilewski, Joseph Murphy, George F. Herlyn, Meenhard Albelda, Steven M.
Internet cím:DOI
elektronikus változat
Borító:
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