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001-es BibID:bibEB00010212
035-os BibID:PMID:7694470
Első szerző:Juhász István (bőrgyógyász, bőrsebész, kozmetológus, klinikai onkológus)
Cím:Regulation of extracellular matrix proteins and integrin cell substratum adhesion receptors on epithelium during cutaneous human wound healing in vivo / Istvan Juhasz, George F. Murphy, Horng-Chin Yan, Meenhard Herlyn, Steven M. Albelda
Dátum:1993
Megjegyzések:Although changes in extracellular matrix proteins during wound healing have been well documented, little is known about the regulation of corresponding extracellular matrix adhesion receptors (integrins). To study this process in a human in vivo model, full thickness human skin grafts were transplanted onto severe combined immunodeficient mice and deep excisional wounds involving both the epidermal and dermal layers were then made. The changes in the expression of cell matrix proteins and epithelial integrins over time were analyzed with specific antibodies using immunohistochemistry. Wounding was associated with alterations in extracellular matrix proteins, namely, loss of laminin and type IV collagen in the region of the wound and expression of tenascin and fibronectin. Changes were also noted in the integrins on the migrating keratinocytes. There was marked up-regulation of the alpha v subunit and de novo expression of the fibronectin receptor (alpha 5 beta 1) during the stage of active migration (days 1 to 3 after wounding). In the later stages of wound healing, after epithelial integrity had been established, redistribution of the alpha 2, alpha 3, alpha 6, and beta 4 collagen/laminin-binding integrin subunits to suprabasal epidermal layers was noted. Thus, during cutaneous wound healing, keratinocytes up-regulate fibronectin/fibrinogen-binding integrins and redistribute collagen/laminin-binding integrins. This study demonstrates that the human skin/severe combined immunodeficient chimera provides a useful model to study events during human wound repair.
Tárgyszavak:idegen nyelvű folyóiratközlemény külföldi lapban
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Megjelenés:American Journal of Pathology. - 143 : 5 (1993), p. 1458-1469. -
További szerzők:Murphy, George F. Yan, Horng-Chin Herlyn, Meenhard Albelda, Steven M.
Internet cím:elektronikus változat
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2.

001-es BibID:bibEBI14267
Első szerző:Pilewski, J. M.
Cím:Modulation of adhesion molecules by cytokines in vivo using human/severe combined immunodeficient (SCID) mouse chimeras / Pilewski, J. M., Yan, H-C., Juhász I., Christofidou-Solomidou, M., Williams, J., Murphy, G. F., Albelda, S. M.
Dátum:1995
Megjegyzések:Endothelial cell-leukocyte interactions involve multiple cell adhesion molecules acting in a programmed and sequential manner to create a leukocyte-endothelial cell adhesion cascade. To understand this process fully, in vivo models are needed. To accomplish this, we have transplanted pieces of normal human tissues onto immunodeficient mice to create chimeric animals. In one model, human skin is grafted and closely resembles normal skin histologically. The grafts retain their human vasculature and show low baseline expression of E-selectin, vascular cell adhesion molecule-1, and intercellular cell adhesion molecule-1. After intradermal injection of human cytokines, these cell adhesion molecules are markedly upregulated and an active inflammatory reaction ensues, with migration of murine leukocytes. Intravenous injection of an anti-human E-selectin antibody completely inhibits leukocyte accumulation induced by tumor necrosis factor-alpha but only partially inhibits leukotriene B4-induced inflammation. In a second model, human bronchus was successfully transplanted heterotopically into severe combined immunodeficient mice. Injection of tumor necrosis factor induced upregulation of E-selectin, intercellular cell adhesion molecule-1, and vascular cell adhesion molecule-1 in the submucosal microvessels, with slightly different kinetics than in the skin. In conclusion, human-severe combined immunodeficient chimeric mice represent a useful model system to study the regulation and function of human cell adhesion molecules in an in vivo setting.
Tárgyszavak:idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:Journal of clinical immunology. - 15 : Suppl.6 (1995), p. 122S-129S. -
További szerzők:Yan, Horng-Chin Juhász István (1956-) (bőrgyógyász, bőrsebész, kozmetológus, klinikai onkológus) Christofidou-Solomidou, M. Williams, J. Murphy, George F. Albelda, Steven M.
Internet cím:DOI
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