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001-es BibID:	bibEB00010210
035-os BibID:	PMID:8342600
Első szerző:	Juhász István (bőrgyógyász, bőrsebész, kozmetológus, klinikai onkológus)
Cím:	Growth and invasion of human melanomas in human skin grafted to immunodeficient mice / Istvan Juhasz, Steven M. Albelda, David E. Elder, George F. Murphy, Koji Adachi, Dorothee Herlyn, Istvan T. Valyi-Nagy, Meenhard Herlyn
Dátum:	1993
Megjegyzések:	An orthotopic model of human melanoma was developed in which malignant cells were injected into human skin grafted to nude and SCID mice. Melanoma cells proliferated and invaded the human skin grafts with characteristic patterns. Three of six melanomas grew as multiple nodules and infiltered the grafts without major architectural changes in the dermis, whereas the others invaded the dermis along collagen fibers with prominent endothelial vessels. By contrast, melanoma cells inoculated into mouse skin grew as diffusely expanding nodules that did not invade the murine dermis. In human skin grafts, human melanoma cells were angiogenic for human blood vessels, and murine vessels were only found at the periphery of grafts. Tumor cells invaded the human vessels, and four out of seven cell lines metastasized to lungs, suggesting that this model is useful to determine in vivo the interactions between normal and malignant human cells.
Tárgyszavak:	idegen nyelvű folyóiratközlemény külföldi lapban külföldön készült közlemény
Megjelenés:	American Journal of Pathology. - 143 : 2 (1993), p. 528-537. -
További szerzők:	Albelda, Steven M. Elder, David E. Murphy, George F. Adachi, Koji Herlyn, Dorothee Vályi-Nagy István Herlyn, Meenhard
Internet cím:	elektronikus változat
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001-es BibID:	bibEBI12846
035-os BibID:	PMID:8478142
Első szerző:	Vályi-Nagy István
Cím:	Spontaneous and induced differentiation of human melanoma cells / Istvan Valyi-Nagy, Ie-Ming Shih, Tibor Gyorfi, David Greenstein, Istvan Juhasz, David E. Elder, Meenhard Herlyn
Dátum:	1993
Megjegyzések:	Malignant melanoma cells can differentiate spontaneously in vivo and in vitro into cells with a finite lifespan. Analysis of differentiating cells from primary melanomas in culture revealed a flat, fibroblast-like morphology and expression of the fibroblast-associated marker leucine aminopeptidase (LAP). Differentiation was also observed in a minor sub-population of permanent cell lines derived from metastatic lesions. An experimental model of melanoma cell differentiation was then developed, using the pyrimidine analog bromodeoxyuridine (BUdR). BUdR-treated cells had a flat morphology, were contact-inhibited, had up to 20-fold increased surface area, expressed LAP, no longer proliferated anchorage-independently in soft agar, and 3 out of 4 cell lines were non-tumorigenic in athymic nude mice. Our results show that models of differentiation of melanoma cells can be established that help to define pathways of differentiation.
Tárgyszavak:	idegen nyelvű folyóiratközlemény külföldi lapban külföldön készült közlemény
Megjelenés:	International Journal of Cancer. - 54 : 1 (1993), p. 159-165. -
További szerzők:	Shih, Ie-Ming Győrfi Tibor Greenstein, David Juhász István (1956-) (bőrgyógyász, bőrsebész, kozmetológus, klinikai onkológus) Elder, David E. Herlyn, Meenhard
Internet cím:	elektronikus változat DOI
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001-es BibID:	bibEBI12844
Első szerző:	Vályi-Nagy István
Cím:	Undifferentiated keratinocytes control growth, morphology, and antigen expression of normal melanocytes through cell-cell contact / Vályi-Nagy, I. T. , Hirka, G., Jensen, P. J., Shih, I. M., Juhász, I., Herlyn, M.
Dátum:	1993
ISSN:	0023-6837 1530-0307
Megjegyzések:	BACKGROUND: Melanocytes in the normal human epidermis are generally dendritic and neither proliferate nor express melanoma-associated antigens. In culture, on the other hand, melanocytes are bi- to tripolar, proliferate with 2 to 4 day doubling times, and express melanoma-associated antigens. This observation prompted us to investigate the regulatory role of keratinocytes for growth, morphology, and antigen expression of melanocytes. EXPERIMENTAL DESIGN: Melanocytes and keratinocytes were cultured under three different co-culture conditions: (a) separated by a semiporous membrane, (b) in monolayer cultures allowing direct contact between cells, and (c) in three-dimensional epidermal reconstructs. RESULTS: Melanocytes separated from keratinocytes by semiporous membranes remained di- and tripolar and could not proliferate in medium optimal for keratinocytes. When cell-cell contact was established between melanocytes and undifferentiated, but not differentiated, keratinocytes, melanocytes proliferated at a rate similar to keratinocytes and they developed multiple dendrites. In co-cultures allowing the multi-layered growth of keratinocytes, melanocytes were nonproliferative when juxtaposed to undifferentiated keratinocytes in the basal layer, but proliferated when surrounded by differentiated keratinocytes in the intermediate and upper layers. Expression of melanoma-associated antigens on melanocytes decreased to similar levels as in normal skin when melanocytes were in direct contact with undifferentiated, but not differentiated, keratinocytes. CONCLUSIONS: Undifferentiated, but not differentiated, keratinocytes control growth, morphology, and antigen expression of melanocytes through direct cell-cell contact. These results suggest that the phenotypic characteristics of nevus and melanoma cells in the dermis, i.e., proliferation and expression of tumor-associated antigens, may be due to their loss of contact with undifferentiation keratinocytes.
Tárgyszavak:	idegen nyelvű folyóiratközlemény külföldi lapban külföldön készült közlemény
Megjelenés:	Laboratory Investigation. - 69 : 2 (1993), p. 152-159. -
További szerzők:	Hirka G. Jensen, P. J. Shih, Ie-Ming Juhász István (1956-) (bőrgyógyász, bőrsebész, kozmetológus, klinikai onkológus) Herlyn, Meenhard
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