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001-es BibID:	BIBFORM019560
Első szerző:	Aleksza Magdolna
Cím:	Increased frequency of intracellular interleukin (IL)-13 and IL-10, but not IL-4, expressing CD4+ and CD8+ peripheral T cells of patients with atopic dermatitis / M. Aleksza, B. Irinyi, A. Lukács, P. Antal-Szalmás, J. Hunyadi, A. Szegedi
Dátum:	2002
Megjegyzések:	Background A number of studies exist demonstrating the increased expression of type 2 cytokinesand decreased capacity to produce interferon-c (IFN-c) in peripheral blood mononuclear cells(PBMCs) of patients with atopic dermatitis (AD).Objectives To clarify the results of recent studies concerning the role of interleukin (IL)-4 and IL-13in PBMCs of AD patients, we analysed the activation status of lymphocyte subpopulations.Methods We measured the intracellular expression and serum levels of certain type 1 and type 2cytokines, using cell surface and intracellular cytokine staining, flow cytometry and enzyme-linkedimmunosorbent assay techniques.Results The frequency of IL-10 and IL-13 producing CD4+ and CD8+ T cells was significantlyhigher in patients with AD, while the frequency of IFN-c secreting helper and cytotoxic T cells wassignificantly lower in patients with AD than in control subjects. The serum levels of IL-10 and IL-13were also significantly increased. There were no significant differences observed between theexperimental groups in the frequency of IL-4 producing CD4+ and CD8+ cells.Conclusions This study demonstrates a type 2 cytokine production in the CD4+ and CD8+ T cellsof AD patients, which is characterized by an elevated IL-13, but not by IL-4 secretion, and by anincreased level of the immunoregulatory IL-10, which can contribute to a decrease in IFN-cexpression.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban atopic dermatitis CD4+ cells CD8+ cells flow cytometry interleukin-4 interleukin-13
Megjelenés:	British Journal of Dermatology. - 147 : 6 (2002), p. 1135-1141. -
További szerzők:	Irinyi Beatrix (1972-) (bőrgyógyász, allergológus) Lukács A. Antal-Szalmás Péter (1968-) (laboratóriumi szakorvos) Hunyadi János (1943-) (bőrgyógyász, kozmetológus, allergológus) Szegedi Andrea (1964-) (bőrgyógyász)
Internet cím:	DOI Intézményi repozitóriumban (DEA) tárolt változat
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001-es BibID:	BIBFORM038886
035-os BibID:	PMID:11903236
Első szerző:	Farkas Beatrix
Cím:	Terbinafine (Lamisil) treatment of toenail onychomycosis in patients with insulin-dependent and non-insulin-dependent diabetes mellitus : a multicentre trial / B. Farkas, C. Paul, A. Dobozy, J. Hunyadi, A. Horvath, G. Fekete
Dátum:	2002
ISSN:	0007-0963
Megjegyzések:	bibEBI12921 újratöltveBackground Diabetes mellitus (DM) affects an estimated 175 million people world-wide.Approximately one-third of patients with DM have toenail onychomycosis.Objectives To determine the ef?cacy and safety of terbina?ne treatment of toenail onychomycosis inpatients with DM receiving insulin and ? or oral antidiabetic agents. Special interest was focused onpotential drug interactions with oral hypoglycaemic substances.Methods In a multicentre trial, patients suffering from insulin-dependent DM (IDDM) or noninsulin-dependent DM (NIDDM) with toenail onychomycosis were treated for 12 weeks with oralterbina?ne 250 mg daily and followed up to 48 weeks. In addition to clinical, mycological andlaboratory investigations, blood glucose levels were monitored.Results At the end of the trial (week 48), a mycological cure rate of 73% was achieved. The ratesof clinical cure and complete cure (mycological cure plus clinical cure) were 57% and 48%,respectively. There was no statistically signi?cant difference between the NIDDM and IDDM groupswith respect to the cure rates (P > 0á05). No hypoglycaemic episode was reported and none of thepatients had hypoglycaemia during the treatment phase.Conclusions With excellent cure rates and a good tolerability pro?le, terbina?ne should continue tobe a drug of choice for the treatment of
Tárgyszavak:	Orvostudományok Egészségtudományok idegen nyelvű folyóiratközlemény külföldi lapban egyetemen (Magyarországon) készült közlemény
Megjelenés:	The British Journal of Dermatology. - 146 : 2 (2002), p. 254-260. -
További szerzők:	Paul, C. Dobozy A. Horváth A. Fekete G. Hunyadi János (1943-) (bőrgyógyász, kozmetológus, allergológus)
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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001-es BibID:	BIBFORM038687
Első szerző:	Gyimesi Edit (klinikai biokémikus, vegyész)
Cím:	Basophil CD63 expression assay on highly sensitized atopic donor leucocytes-a useful method in chronic autoimmune urticaria / E. Gyimesi, S. Sipka, K. Dankó, E. Kiss, B. Hídvégi, M. Gál, J. Hunyadi, B. Irinyi, A. Szegedi
Dátum:	2004
ISSN:	0007-0963
Megjegyzések:	AbstractBACKGROUND: The autoimmune subclass of chronic idiopathic urticaria (CU) has been characterized by the occurrence of biologically relevant IgG antibodies against the IgE molecule or the alpha chain of the high-affinity Fcepsilon receptor (FcepsilonRIalpha) on basophils and mast cells. These antibodies are usually detected by autologous serum skin testing and confirmed by histamine release studies, immunoblotting, or enzyme-linked immunosorbent assay, but not always.OBJECTIVES: To detect autoantibodies to the FcepsilonRIalpha in sera of CU patients by a modified serum-induced basophil activation test measured by flow cytometry (FCM) and to evaluate the relationship between the in vitro functional test, the autologous serum skin test (ASST), and the serum levels of IgE, eosinophil cationic protein (ECP) and antithyroid antibodies.METHODS: Sera of 30 patients with CU and 26 patients with systemic autoimmune diseases (systemic lupus erythematosus, dermatomyositis) were tested for CD63 activation marker expression on basophils by FCM. Leucocytes from two highly sensitized atopic donors (D(A1,) D(A2)) and one non-atopic donor (D(NA)) were incubated with patients' sera and double-labelled with anti-IgE and anti-CD63 antibodies. Subsequently, the percentage of CD63-expressing basophils was determined by using FCM. In all CU patients an ASST was carried out and the serum IgE, and ECP levels and antithyroid antibodies were evaluated.RESULTS: Twelve patients had a positive ASST and 14 patients a positive CD63 expression assay. There was a strong correlation between the ASST and CD63 assay. Sera from patients with systemic autoimmune diseases did not raise positive CD63 expression on basophils. There was a moderate negative correlation between the occurrence of atopic serum markers (IgE, ECP) and the ability of sera to induce CD63 expression on basophil cells of D(A2) (P < 0.05). The female sex was preponderant and antithyroid antibodies were more frequent.CONCLUSIONS: Our new technical observation demonstrates that basophils of highly sensitized atopic donors can be successfully used without priming with IL-3 for the in-vitro flow cytofluorimetric diagnosis of CU. With this investigation the characterization of the autoimmune origin of CU is based on an objective in vitro technique.
Tárgyszavak:	Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban autoimmune urticaria CD63 assay basophil diagnosis egyetemen (Magyarországon) készült közlemény
Megjelenés:	British Journal of Dermatology. - 151 : 2 (2004), p. 388-396. -
További szerzők:	Sipka Sándor (1945-) (laboratóriumi szakorvos) Dankó Katalin (1952-2021) (belgyógyász, allergológus és klinikai immunológus) Kiss Emese (1960-) (belgyógyász, immunológus) Hidvégi Bernadett Gál Mónika Hunyadi János (1943-) (bőrgyógyász, kozmetológus, allergológus) Irinyi Beatrix (1972-) (bőrgyógyász, allergológus) Szegedi Andrea (1964-) (bőrgyógyász)
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001-es BibID:	BIBFORM023739
Első szerző:	Szegedi Andrea (bőrgyógyász)
Cím:	Significant correlation between the CD63 assay and the histamine release assay in chronic urticaria / A. Szegedi, B. Irinyi, M. Gál, J. Hunyadi, K. Dankó, E. Kiss, S. Sipka, G. Szegedi, E. Gyimesi
Dátum:	2006
Megjegyzések:	Background Antibodies directed to the a subunit of the high affinity IgE receptorand the IgE molecule are proposed to be of pathogenetic relevance in a group ofpatients with chronic urticaria (CU). The diagnosis of autoimmune chronic urticaria(ACU) is difficult; the autologous serum skin test (ASST) seems to be a usefulscreening test, but reliable, additional confirmatory methods are needed.Objectives To assess the diagnostic value of a modified serum-induced basophilactivation test, the CD63 expression assay, in the diagnosis of ACU by comparingthe results of the CD63 assay with the results of the histamine release (HR) test,the ASST and serum levels of soluble CD40 ligand (sCD40L).Methods Using basophils from an atopic (DA) and a nonatopic (DNA) donor theactivity of sera of 72 patients with CU were measured in HR assay by enzymelinkedimmunosorbent assay and in CD63 expression assay by flow cytometry.An ASST was carried out in all patients; in 30 of the 72 patients sCD40L wasdetected and correlations were derived between the different assays. Sera of 20normal controls and 26 patients with systemic autoimmune diseases were alsotested in the HR assay and in the CD63 expression assay.Results Histamine-releasing activity was detected in the sera of 51% (DA) and 32%(DNA) of CU patients and 57% (DA) and 28% (DNA) of sera upregulated CD63expression on the surface of basophils from the different donors. There was asignificant correlation between the HR and the CD63 assays carried out on bothdonors, but the ASST showed a strong correlation with the HR assay only for basophilsfrom the DA. The serum level of sCD40L was significantly higher inpatients with CU compared with controls, but the difference between the autoimmuneand the nonautoimmune groups was not significant.Conclusions The CD63 expression assay seems to be a reliable functional test in thediagnosis of ACU, particularly if highly sensitive donor basophils are used, butthe determination of the sCD40L serum level was not sufficient to differentiate
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban CD63 assay chronic urticaria flow cytometry
Megjelenés:	British Journal of Dermatology. - 155 : 1 (2006), p. 67-75. -
További szerzők:	Irinyi Beatrix (1972-) (bőrgyógyász, allergológus) Gál Mónika Hunyadi János (1943-) (bőrgyógyász, kozmetológus, allergológus) Dankó Katalin (1952-2021) (belgyógyász, allergológus és klinikai immunológus) Kiss Emese (1960-) (belgyógyász, immunológus) Sipka Sándor (1945-) (laboratóriumi szakorvos) Szegedi Gyula (1936-2013) (belgyógyász, immunológus) Gyimesi Edit (1957-) (klinikai biokémikus, vegyész)
Internet cím:	DOI Intézményi repozitóriumban (DEA) tárolt változat
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