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001-es BibID:	BIBFORM014256
Első szerző:	Bakondi Edina (biokémikus, vegyész)
Cím:	Detection of poly(ADP-ribose) polymerase activation in oxidatively stressed cells and tissues using biotinylated NAD substrate / Bakondi, E., Bai, P., Szabo, E., Hunyadi, J., Gergely, P., Szabo, C., Virag, L.
Dátum:	2002
ISSN:	0022-1554 (Print)
Megjegyzések:	Poly(ADP-ribose) polymerase (PARP) is a nuclear enzyme activated by DNA damage. Activated PARP cleaves NAD(+) into nicotinamide and (ADP-ribose) and polymerizes the latter on nuclear acceptor proteins. Over-activation of PARP by reactive oxygen and nitrogen intermediates represents a pathogenetic factor in various forms of inflammation, shock, and reperfusion injury. Using a novel commercially available substrate, 6-biotin-17-nicotinamide-adenine-dinucleotide (bio-NAD(+)), we have developed three applications, enzyme cytochemistry, enzyme histochemistry, and cell ELISA, to detect the activation of PARP in oxidatively stressed cells and tissues. With the novel assay we were able to detect basal and hydrogen peroxide-induced PARP activity in J774 macrophages. We also observed that mitotic cells display remarkably elevated PARP activity. Hydrogen peroxide-induced PARP activation could also be detected in wild-type peritoneal macrophages but not in macrophages from PARP-deficient mice. Application of hydrogen peroxide to the skin of mice also induced bio-NAD(+) incorporation in the keratinocyte nuclei. Hydrogen peroxide-induced PARP activation and its inhibition by pharmacological PARP inhibitors could be detected in J774 cells with the ELISA assay that showed good correlation with the traditional [(3)H]-NAD incorporation method. The bio-NAD(+) assays represent sensitive, specific, and non-radioactive alternatives for detection of PARP activation.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban Animals Biotin/analogs & derivatives Cells, Cultured egyetemen (Magyarországon) készült közlemény Enzyme Activation Enzyme-Linked Immunosorbent Assay Hydrogen Peroxide Macrophages Mice Mice, Inbred C57BL Mice, Mutant Strains NAD/analogs & derivatives Oxidative Stress Poly(ADP-ribose) Polymerases Skin/drug effects
Megjelenés:	The Journal of Histochemistry and Cytochemistry. - 50 : 1 (2002), p. 91-98. -
További szerzők:	Bai Péter (1976-) (biokémikus) Szabó Éva (1965-) (bőrgyógyász, kozmetológus) Hunyadi János (1943-) (bőrgyógyász, kozmetológus, allergológus) Gergely Pál (1947-) (biokémikus) Szabó Csaba Virág László (1965-) (biokémikus, sejtbiológus, farmakológus)
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001-es BibID:	BIBFORM039793
035-os BibID:	PMID:12102657
Első szerző:	Virág László (biokémikus, sejtbiológus, farmakológus)
Cím:	Nitric oxide-peroxynitrite-poly(ADP-ribose) polymerase pathway in the skin / László Virág, Éva Szabó, Edina Bakondi, Péter Bai, Pál Gergely, János Hunyadi, Csaba Szabo
Dátum:	2002
ISSN:	0906-6705
Megjegyzések:	In the last decade it has become well established that in the skin, nitric oxide (NO), a diffusable gas, mediates various physiologic functions ranging from the regulation of cutaneous blood flow to melanogenesis. If produced in excess, NO combines with superoxide anion to form peroxynitrite (ONOO-), a cytotoxic oxidant that has been made responsible for tissue injury during shock, inflammation and ischemia-reperfusion. The opposite effects of NO and ONOO- on various cellular processes may explain the 'double-edged sword' nature of NO depending on whether or not cellular conditions favour peroxynitrite formation. Peroxynitrite has been shown to activate the nuclear nick sensor enzyme, poly(ADP-ribose) polymerase (PARP). Overactivation of PARP depletes the cellular stores of NAD+, the substrate of PARP, and the ensuing 'cellular energetic catastrophy' results in necrotic cell death. Whereas the role of NO in numerous skin diseases including wound healing, burn injury, psoriasis, irritant and allergic contact dermatitis, ultraviolet (UV) light-induced sunburn erythema and the control of skin infections has been extensively documented, the intracutaneous role of peroxynitrite and PARP has not been fully explored. We have recently demonstrated peroxynitrite production, DNA breakage and PARP activation in a murine model of contact hypersensitivity, and propose that the peroxynitrite-PARP route represents a common pathway in the pathomechanism of inflammatory skin diseases. Here we briefly review the role of NO in skin pathology and focus on the possible roles played by peroxynitrite and PARP in various skin diseases.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban egyetemen (Magyarországon) készült közlemény
Megjelenés:	Experimental Dermatology 11 : 3 (2002), p. 189-202. -
További szerzők:	Szabó Éva (1965-) (bőrgyógyász, kozmetológus) Bakondi Edina (1975-) (biokémikus, vegyész) Bai Péter (1976-) (biokémikus) Gergely Pál (1947-) (biokémikus) Hunyadi János (1943-) (bőrgyógyász, kozmetológus, allergológus) Szabó Csaba
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