CCL

Összesen 4 találat.
#/oldal:
Részletezés:
Rendezés:

1.

001-es BibID:BIBFORM045939
035-os BibID:PMID:12839568
Első szerző:Bakondi Edina (biokémikus, vegyész)
Cím:Role of Intracellular Calcium Mobilization and Cell-Density-Dependent Signaling in Oxidative-Stress-Induced Cytotoxicity in HaCaT Keratinocytes / Edina Bakondi, Mónika Gönczi, Éva Szabó, Péter Bai, Pál Pacher, Pál Gergely, László Kovács, János Hunyadi, Csaba Szabó, László Csernoch, László Virág
Dátum:2003
ISSN:0022-202X
Megjegyzések:Peroxynitrite is a nitric-oxide-derived cytotoxic mediator produced in a broad range of inflammatory conditions, ranging from sunburn erythema to contact hypersensitivity. Our previous work has shown that in HaCaT cells the cytotoxic activity of peroxynitrite involves both apoptotic and necrotic routes with poly(ADP-ribose) polymerase activation serving as a mol-ecular switch diverting the default apoptotic pathway toward necrosis. Nonetheless, keratinocytes are regarded as highly resistant toward environmental noxa including oxidative stress. We set out to investigate the possible role of two parameters, intracellular calcium mobilization and high cell density, in protecting HaCaT cells from peroxynitrite/oxidative-stress-induced cytotoxicity. First we characterized the effect of peroxynitrite on the calcium homeostasis of HaCaT cells and demonstrated that both authentic peroxynitrite and the peroxynitrite generating compound 3-morpholino-sydnonimine triggered an elevation in intracellular calcium levels. Moreover, we established that treatment of cells with the cell-permeable calcium chelator BAPTA-AM provided significant cytoprotection against peroxynitrite- and hydrogen-peroxide-induced cytotoxicity. Furthermore, when cells reached confluence they were highly resistant to the toxic effects of peroxynitrite, hydrogen peroxide, and superoxide. The resistance to oxidative stress provided by calcium chelation and high cell density involved inhibiting the activation of both poly(ADP-ribose) polymerase and caspases. Our data may provide an explanation for the resistance to oxidative stress of superficial, highly differentiated keratinocytes and indicate that basal proliferative keratinocytes are sensitive in vivo targets of oxidative stress injury.
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
egyetemen (Magyarországon) készült közlemény
Megjelenés:Journal of Investigative Dermatology. - 121 : 1 (2003), p. 88-95. -
További szerzők:Gönczi Mónika (1974-) (élettanász) Szabó Éva (1965-) (bőrgyógyász, kozmetológus) Bai Péter (1976-) (biokémikus) Pacher Pál Gergely Pál (1947-) (biokémikus) Kovács László (1939-) (élettanász) Hunyadi János (1943-) (bőrgyógyász, kozmetológus, allergológus) Szabó Csaba (1967-) (orvos) Csernoch László (1961-) (élettanász) Virág László (1965-) (biokémikus, sejtbiológus, farmakológus)
Internet cím:Szerző által megadott URL
DOI
Intézményi repozitóriumban (DEA) tárolt változat
Borító:

2.

001-es BibID:BIBFORM014256
Első szerző:Bakondi Edina (biokémikus, vegyész)
Cím:Detection of poly(ADP-ribose) polymerase activation in oxidatively stressed cells and tissues using biotinylated NAD substrate / Bakondi, E., Bai, P., Szabo, E., Hunyadi, J., Gergely, P., Szabo, C., Virag, L.
Dátum:2002
ISSN:0022-1554 (Print)
Megjegyzések:Poly(ADP-ribose) polymerase (PARP) is a nuclear enzyme activated by DNA damage. Activated PARP cleaves NAD(+) into nicotinamide and (ADP-ribose) and polymerizes the latter on nuclear acceptor proteins. Over-activation of PARP by reactive oxygen and nitrogen intermediates represents a pathogenetic factor in various forms of inflammation, shock, and reperfusion injury. Using a novel commercially available substrate, 6-biotin-17-nicotinamide-adenine-dinucleotide (bio-NAD(+)), we have developed three applications, enzyme cytochemistry, enzyme histochemistry, and cell ELISA, to detect the activation of PARP in oxidatively stressed cells and tissues. With the novel assay we were able to detect basal and hydrogen peroxide-induced PARP activity in J774 macrophages. We also observed that mitotic cells display remarkably elevated PARP activity. Hydrogen peroxide-induced PARP activation could also be detected in wild-type peritoneal macrophages but not in macrophages from PARP-deficient mice. Application of hydrogen peroxide to the skin of mice also induced bio-NAD(+) incorporation in the keratinocyte nuclei. Hydrogen peroxide-induced PARP activation and its inhibition by pharmacological PARP inhibitors could be detected in J774 cells with the ELISA assay that showed good correlation with the traditional [(3)H]-NAD incorporation method. The bio-NAD(+) assays represent sensitive, specific, and non-radioactive alternatives for detection of PARP activation.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Animals
Biotin/analogs & derivatives
Cells, Cultured
egyetemen (Magyarországon) készült közlemény
Enzyme Activation
Enzyme-Linked Immunosorbent Assay
Hydrogen Peroxide
Macrophages
Mice
Mice, Inbred C57BL
Mice, Mutant Strains
NAD/analogs & derivatives
Oxidative Stress
Poly(ADP-ribose) Polymerases
Skin/drug effects
Megjelenés:The Journal of Histochemistry and Cytochemistry. - 50 : 1 (2002), p. 91-98. -
További szerzők:Bai Péter (1976-) (biokémikus) Szabó Éva (1965-) (bőrgyógyász, kozmetológus) Hunyadi János (1943-) (bőrgyógyász, kozmetológus, allergológus) Gergely Pál (1947-) (biokémikus) Szabó Csaba Virág László (1965-) (biokémikus, sejtbiológus, farmakológus)
Internet cím:elektronikus változat
DOI
Intézményi repozitóriumban (DEA) tárolt változat
Borító:

3.

001-es BibID:BIBFORM028552
Első szerző:Szabó Éva (bőrgyógyász, kozmetológus)
Cím:Peroxynitrite production, DNA breakage, and poly(ADP-ribose) polymerase activation in a mouse model of oxazolone-induced contact hypersensitivity / Éva Szabó, László Virág, Edina Bakondi, László Gyüre, György Haskó, Péter Bai, János Hunyadi, Pál Gergely, Csaba Szabó
Dátum:2001
Megjegyzések:Peroxynitrite-induced poly(ADP-ribose) polymerase activation has been implicated in the pathogenesis of various inflammatory conditions. Here we have investigated whether peroxynitrite and poly(ADP-ribose) polymerase may play a role in the pathophysiology of the elicitation phase of contact hypersensitivity. We have detected nitrotyrosine, DNA breakage, and poly(ADP-ribose) polymerase activation in the epidermis of mice in an oxazolone-induced contact hypersensitivity model. As tyrosine nitration is mostly mediated by peroxynitrite, a nitric-oxide-derived cytotoxic oxidant capable of causing DNA breakage, we have applied peroxynitrite directly on mouse skin and showed poly(ADP-ribose) polymerase activation in keratinocytes and in some scattered dermal cells. We have also investigated the cellular effects of peroxynitrite in HaCaT cells, a human keratinocyte cell line. We found that peroxynitrite inhibited cell proliferation and at higher concentrations also caused cytotoxicity. Peroxynitrite activates poly(ADP-ribose) polymerase in HaCaT cells and poly(ADP-ribose) polymerase activation contributes to peroxynitrite-induced cytotoxicity, as indicated by the cytoprotective effect of the poly(ADP-ribose) polymerase inhibitor 3-aminobenzamide. The cytoprotective effect of 3-aminobenzamide cannot be attributed to inhibition of apoptosis, as apoptotic parameters (caspase activation and DNA fragmentation) were not reduced in the presence of 3-aminobenzamide in peroxynitrite-treated cells. Moreover, poly(ADP-ribose) polymerase inhibition by 3-aminobenzamide dose-dependently reduced interferon-induced intercellular adhesion molecule 1 expression as well as interleukin-1beta-induced interleukin-8 expression. Our results indicate that peroxynitrite and poly(ADP-ribose) polymerase regulate keratinocyte function and death in contact hypersensitivity.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
egyetemen (Magyarországon) készült közlemény
Megjelenés:Jounal of Investigative Dermatoloty. - 117 : 1 (2001), p. 74-80. -
További szerzők:Virág László (1965-) (biokémikus, sejtbiológus, farmakológus) Bakondi Edina (1975-) (biokémikus, vegyész) Gyüre László Haskó György (1967-) (biokémikus) Bai Péter (1976-) (biokémikus) Hunyadi János (1943-) (bőrgyógyász, kozmetológus, allergológus) Gergely Pál (1947-) (biokémikus) Szabó Csaba (1967-) (orvos)
Internet cím:Intézményi repozitóriumban (DEA) tárolt változat
DOI
Borító:

4.

001-es BibID:BIBFORM039793
035-os BibID:PMID:12102657
Első szerző:Virág László (biokémikus, sejtbiológus, farmakológus)
Cím:Nitric oxide-peroxynitrite-poly(ADP-ribose) polymerase pathway in the skin / László Virág, Éva Szabó, Edina Bakondi, Péter Bai, Pál Gergely, János Hunyadi, Csaba Szabo
Dátum:2002
ISSN:0906-6705
Megjegyzések:In the last decade it has become well established that in the skin, nitric oxide (NO), a diffusable gas, mediates various physiologic functions ranging from the regulation of cutaneous blood flow to melanogenesis. If produced in excess, NO combines with superoxide anion to form peroxynitrite (ONOO-), a cytotoxic oxidant that has been made responsible for tissue injury during shock, inflammation and ischemia-reperfusion. The opposite effects of NO and ONOO- on various cellular processes may explain the 'double-edged sword' nature of NO depending on whether or not cellular conditions favour peroxynitrite formation. Peroxynitrite has been shown to activate the nuclear nick sensor enzyme, poly(ADP-ribose) polymerase (PARP). Overactivation of PARP depletes the cellular stores of NAD+, the substrate of PARP, and the ensuing 'cellular energetic catastrophy' results in necrotic cell death. Whereas the role of NO in numerous skin diseases including wound healing, burn injury, psoriasis, irritant and allergic contact dermatitis, ultraviolet (UV) light-induced sunburn erythema and the control of skin infections has been extensively documented, the intracutaneous role of peroxynitrite and PARP has not been fully explored. We have recently demonstrated peroxynitrite production, DNA breakage and PARP activation in a murine model of contact hypersensitivity, and propose that the peroxynitrite-PARP route represents a common pathway in the pathomechanism of inflammatory skin diseases. Here we briefly review the role of NO in skin pathology and focus on the possible roles played by peroxynitrite and PARP in various skin diseases.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
egyetemen (Magyarországon) készült közlemény
Megjelenés:Experimental Dermatology 11 : 3 (2002), p. 189-202. -
További szerzők:Szabó Éva (1965-) (bőrgyógyász, kozmetológus) Bakondi Edina (1975-) (biokémikus, vegyész) Bai Péter (1976-) (biokémikus) Gergely Pál (1947-) (biokémikus) Hunyadi János (1943-) (bőrgyógyász, kozmetológus, allergológus) Szabó Csaba
Internet cím:Szerző által megadott URL
DOI
Intézményi repozitóriumban (DEA) tárolt változat
Borító:
Rekordok letöltése1