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1.

001-es BibID:BIBFORM004135
Első szerző:Cseri Julianna (laboratóriumi diagnosztika szakorvos)
Cím:A purinergic signal transduction pathway in mammalian skeletal muscle cells in culture / Julianna Cseri, Henrietta Szappanos, Gyula Péter Szigeti, Zoltán Csernátony, László Kovács, László Csernoch
Dátum:2002
ISSN:0031-6768
Megjegyzések:The effects of adenosine 5'-triphosphate (ATP) on human and mouse skeletal muscle fibres in primary culture were investigated. ATP-evoked changes in intracellular calcium concentration ([Ca(2+)](i)) were measured and compared with those induced by agonists of the nicotinic acetylcholine (Ach)- and P2X purinoreceptors. While ATP was effective on both myoblasts and multi-nucleated myotubes in the micromolar range, Ach failed to induce any change in [Ca(2+)](i) at early stages of development. In contrast, myofibres with peripheral nuclei showed little response to ATP but responded to Ach with a large change in [Ca(2+)](i). The responsiveness of the myotubes to Ach paralleled that to potassium. The removal of external calcium abolished the response to ATP. P2X receptor agonists mimicked the response to ATP with the order of potency being ATP>2',3'- O-(4-benzoyl)-benzoyl-ATP>beta,gamma-methylene-ATP>alpha,beta-methylene-ATP. Under voltage-clamp conditions ATP induced an inward current that showed little inactivation. These results are consistent with the existence of P2X receptor-mediated signal transduction pathway in cultured mammalian skeletal muscle cells.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:Pflügers Archiv. - 443 : 5-6 (2002), p. 731-738. -
További szerzők:Szappanos Henrietta (1976-) (biológus, élettanász) Szigeti Gyula (1969-) (élettanász, elektrofiziológus) Csernátony Zoltán (1959-2023) (ortopéd sebész, traumatológus) Kovács László (1939-) (élettanász) Csernoch László (1961-) (élettanász)
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DOI
Intézményi repozitóriumban (DEA) tárolt változat
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2.

001-es BibID:bibEBI00019167
Első szerző:Deli Tamás (szülész-nőgyógyász, endokrinológus szakorvos)
Cím:Contribution from P2X and P2Y purinoreceptors to ATP-evoked changes in intracellular calcium concentration on cultured myotubes / Tamás Deli, Henrietta Szappanos, Gyula Péter Szigeti, Julianna Cseri, László Kovács, László Csernoch
Dátum:2007
ISSN:0031-6768
Megjegyzések:Although the alteration of purinoreceptor pattern on skeletal muscle is known to accompany physiological muscle differentiation and the pathogenesis of muscle dystrophy, the exact identity of and the relative contribution from the individual receptor subtypes to the purinergic signal have been controversial. To identify these subtypes in cultured myotubes of 5-10 nuclei, changes in intracellular calcium concentration and surface membrane ionic currents were detected and calcium fluxes calculated after the application of the subtype-specific agonists 2'3'-O-(benzoyl-4-benzoyl)-ATP (BzATP), 2-methyltio-ADP and UTP. The effectiveness of these agonists together with positive immunocytochemical staining revealed the presence of P2X(4), P2X(5), P2X(7), P2Y(1) and P2Y(4) receptors. siRNA-reduced protein expression of P2X(5), P2X(7) and P2Y(1) receptors was accompanied by reduction in the ATP-evoked calcium transients. Furthermore, anti-P2X(7) siRNA caused a significant drop in the early peak and delayed steady component of the calculated calcium flux. The use of its antagonist, oxidized ATP, similarly to transfection with anti-P2X(7) siRNA caused significant reduction in the agonist-elicited ionic currents I (ATP) and I (BzATP), with a greater drop in the latter. Our results demonstrate that the activation of ionotropic P2X(4), P2X(5) and P2X(7) and metabotropic P2Y(1) and P2Y(4) purinoreceptors participates in forming the calcium transients of multinucleated myotubes.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:Pflügers Archiv. - 453 : 4 (2007), p. 519-529. -
További szerzők:Szappanos Henrietta (1976-) (biológus, élettanász) Szigeti Gyula (1969-) (élettanász, elektrofiziológus) Cseri Julianna (1949-2022) (laboratóriumi diagnosztika szakorvos) Kovács László (1939-) (élettanász) Csernoch László (1961-) (élettanász)
Internet cím:Intézményi repozitóriumban (DEA) tárolt változat
DOI
Szerző által megadott URL
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3.

001-es BibID:BIBFORM001402
Első szerző:Szigeti Gyula (élettanász, elektrofiziológus)
Cím:Differentiation-dependent alterations in the extracellular ATP-evoked calcium fluxes of cultured skeletal muscle cells from mice / Gyula Péter Szigeti, Henrietta Szappanos, Tamás Deli, Julianna Cseri, László Kovács, László Csernoch
Dátum:2007
ISSN:0031-6768
Megjegyzések:Although extracellular adenosine triphosphate (ATP) has been generally accepted as the regulator of cellular differentiation, the relative contribution of the various purinoreceptor subtypes to purinergic signalling at distinct stages of skeletal muscle differentiation is still poorly understood. Here we measured extracellular ATP-evoked changes in intracellular calcium concentration and surface membrane ionic currents (I (ATP)), calculated the calcium flux (FL) entering the myoplasmic space and compared these parameters at different stages of differentiation on cultured mouse myotubes. The ATP-evoked FL displayed an early peak and then declined to a steady level. With differentiation, the early peak became separated from the maintained component and was absent on mature myotubes. Repeated ATP applications caused desensitization of the response in both immature and differentiated myotubes, owing mainly to the reduction of the early peak of FL in the former and to a decline of both components in the latter group of cells. Depolarization of the cell or removal of external calcium suppressed the early peak. I (ATP) showed no inactivation, and its voltage dependence displayed strong inward rectification. The concentration dependence of I (ATP) can be fitted using a Hill equation, yielding an EC(50) of 56 microM. Results are consistent with the parallel activation of both P2X and P2Y receptors.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:Pflügers Archiv. - 453 : 4 (2007), p. 509-518. -
További szerzők:Deli Tamás (1979-) (szülész-nőgyógyász, endokrinológus szakorvos) Cseri Julianna (1949-2022) (laboratóriumi diagnosztika szakorvos) Kovács László (1939-) (élettanász) Csernoch László (1961-) (élettanász) Szappanos Henrietta (1976-) (biológus, élettanász)
Internet cím:Intézményi repozitóriumban (DEA) tárolt változat
DOI
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4.

001-es BibID:BIBFORM001082
Első szerző:Szigeti Gyula (élettanász, elektrofiziológus)
Cím:Alterations in the calcium homeostasis of skeletal muscle from postmyocardial infarcted rats / Gyula Péter Szigeti, János Almássy, Mónika Sztretye, Beatrix Dienes, László Szabó, Péter Szentesi, Guy Vassort, Sándor Sárközi, László Csernoch, István Jóna
Dátum:2007
ISSN:0031-6768
Megjegyzések:In chronic heart failure, skeletal muscles develop a weakness that is not associated to an impaired circulatory function but rather to alterations in the skeletal muscle fibers themselves. To understand these changes, the steps in excitation-contraction coupling of rats that underwent a left anterior coronary artery occlusion were studied. About 24 weeks after the myocardial infarction, neither the total amount nor the voltage dependence of intramembrane charge were altered. In contrast, calcium release from the sarcoplasmic reticulum was considerably suppressed, and its voltage dependence shifted toward more positive voltages. Elementary calcium-release events showed altered morphology as the relative proportion of embers increased. Calcium sparks were smaller in amplitude and had larger time-to-peak values. Isolated ryanodine receptors (RyR) displayed an unusual rectification with increased single-channel conductance at positive (cis vs trans) voltages. In addition, the bell-shaped calcium dependence of channel activity was broader, with a slight shift of activation to lower and a larger shift in inactivation to higher calcium concentrations. These data indicate that the number of channels that open during a calcium-release event is decreased and that RyR function is altered; thus, calcium-release is suppressed after a myocardial infarction. These observations give an explanation for the impaired skeletal muscle function in these animals.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:Pflügers Archiv. - 455 : 3 (2007), p. 541-553. -
További szerzők:Almássy János (1981-) (élettanász, biológus, angol-magyar szakfordító) Sztretye Mónika (1981-) (élettanász, elektrofiziológus) Dienes Beatrix (1972-) (élettanász, molekuláris biológus) Szabó László (orvos) Szentesi Péter (1967-) (élettanász) Vassort, Guy Sárközi Sándor (1966-) (élettanász) Csernoch László (1961-) (élettanász) Jóna István (1948-) (élettanász, fizikus)
Internet cím:Intézményi repozitóriumban (DEA) tárolt változat
DOI
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5.

001-es BibID:BIBFORM005104
Első szerző:Szűcs Attila (fül-orr-gégész)
Cím:Differential expression of potassium currents in Deiters cells of the guinea pig cochlea / Attila Szűcs, Sándor Somodi, Tamás J. Batta, Andrea Tóth, Gyula P. Szigeti, László Csernoch, György Panyi, István Sziklai
Dátum:2006
ISSN:0031-6768
Megjegyzések:Among the supporting cells, Deiters cells are in intimate contact with outer hair cells (OHCs) in the inner ear. The aim of this study was to characterize the outward rectifying K+ current of Deiters cells in conjunction with cellular morphological characteristics. In the majority of cells, the K+ current had a biphasic inactivation kinetics (tau1 and tau2 were 2,735+/-90 (n=77) and 160+/-14 ms (n=72), respectively). The rapidly inactivating current component was more sensitive to Charybdotoxin (ChTx, 10 nM) block whereas the slowly inactivating current could be blocked more efficiently by tetraethylammonium (1 mM). All these point toward the existence of two distinct potassium channel types in these cells. Deiters cells attached to shorter OHCs had more voluminous, whereas those attached to longer OHCs had lanky cell bodies. The inactivation kinetics was slower in cells having corpulent cell bodies due to the increased proportion of the slowly inactivating current component (0.736+/-0.033, n=27) as compared to the one determined for lanky cells (0.522+/-0.023, n=36). The average peak K+ current was higher in Deiters cells connected to OHCs (5,417+/-541 pA, n=40) than in isolated ones (3,527+/-410, n=37). Deiters cells having different cell shapes and showing different K+ channel expression may contribute to the active mechanism of the cochlea to various degrees.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Animals
Cells
Charybdotoxin
cytology
Guinea Pigs
Hungary
Kinetics
metabolism
Organ of Corti
Patch-Clamp Techniques
Potassium
Potassium Channel Blockers
Potassium Channels
Research
Support
Tetraethylammonium
Megjelenés:Pflügers Archiv. - 452 : 3 (2006), p. 332-341. -
További szerzők:Somodi Sándor (1977-) (belgyógyász) Batta József Tamás (1970-) (fül-orr-gégész) Tóth Andrea (1973-) (fül-orr-gégész) Szigeti Gyula (1969-) (élettanász, elektrofiziológus) Csernoch László (1961-) (élettanász) Panyi György (1966-) (biofizikus) Sziklai István (1954-) (fül-orr-gégész)
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