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001-es BibID:BIBFORM009078
Első szerző:Lai, Henry H.
Cím:Activation of cholinergic receptors blocks non-adrenergic non-cholinergic contractions in the rat urinary bladder / Lai, H. H., Smith, C. P., Munoz, A., Boone, T. B., Szigeti, G. P., Somogyi, G. T.
Dátum:2008
ISSN:1873-2747 (Electronic)
Megjegyzések:In the present study, the plasticity of the non-adrenergic non-cholinergic (NANC) response was investigated. Isolated rat bladder strips were electrically stimulated and the evoked contractions were isometrically recorded. The NANC part of the contractions were unmasked by applying 500 nM 4-DAMP, a potent muscarinic antagonist. Treatment of the bladder strips with 10 microM carbachol (a cholinergic agonist) increased the muscle tone but did not alter the neurally evoked contractions. However, carbachol decreased: (1) the NANC response from 74.6% to 33.3% of control and (2) the purinergic contractile response to alpha,beta-methylene ATP (alpha,beta-mATP) (10 microM) from 97.0% to 43.4% (p<0.05). Treatment with the cholinesterase inhibitor eserine (10 microM) also significantly decreased the NANC response to 21.1% (p<0.0001). The purinergic receptor antagonist suramin (100 microM) did not affect the neurally evoked contractions, however; subsequent addition of 4-DAMP decreased the contractions to 31%. Activation of the smooth muscle cholinergic receptors (with carbachol or eserine) and purinergic receptors (with alpha,beta-mATP) decreased the NANC contractions and the direct contractile response to alpha,beta-mATP. When the electrically evoked contractions were facilitated by the L-type Ca2+ channel activator, Bay-K 8644 the subsequent application of 4-DAMP did not unmask inhibited NANC contractions. We conclude that activation of muscarinic receptors by cholinergic agonist, carbachol or by endogenous acetylcholine (ACh) induce a cascade of events that leads to diminished purinergic response and consequently an inhibition of the bladder NANC response.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
3-Pyridinecarboxylic acid,
1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl
ester
Adenosine Triphosphate
Animals
Antineoplastic Agents
Atropine
Calcium Channel Agonists
Carbachol
Cholinergic Agonists
Cholinesterase Inhibitors
Electric Stimulation
Female
Muscarinic Antagonists
Muscle Contraction
Muscle Relaxation
Muscle, Smooth
Physostigmine
Piperidines
Rats
Rats, Sprague-Dawley
Receptors, Cholinergic
Suramin
Urinary Bladder
Megjelenés:Brain Research Bulletin. - 77 (2008), p. 420-426. -
További szerzők:Smith, Christopher P. Munoz, Alvaro Boone, Timothy B. Szigeti Gyula (1969-) (élettanász, elektrofiziológus) Somogyi George T.
Internet cím:DOI
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