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001-es BibID:	BIBFORM034509
035-os BibID:	PMID:7545323 WOS:A1995QM81900034
Első szerző:	Crawford, Neville
Cím:	Targeting platelets containing electro-encapsulated iloprost to balloon injured aorta in rats / N. Crawford, A. Chajara, G. Pfliegler, B. El. Gamal, L. Brewer, L. Capron
Dátum:	1995
ISSN:	0340-6245
Megjegyzések:	Drugs can be electro-encapsulated within platelets and targeted to damaged blood vessels by exploiting the platelet's natural haemostatic properties to adhere to collagen and other vessel wall constituents revealed by injury. A rat aorta balloon angioplasty model has been used to study the effect on platelet deposition of giving iloprost loaded platelets i.v. during the balloon injury. After labelling the circulating platelets with 111-Indium before balloon injury, time course studies showed maximum platelet deposition on the injured aorta occurred at about 1 h post-injury and the deposition remained stable over the next 2-3 h. When iloprost-loaded platelets were given i.v. during injury and the circulating platelet pool labelled with 111-Indium 30 min later, platelet deposition, measured at 2 h postinjury, was substantially and significantly reduced compared with control platelet treatment. Some anti-proliferative effects of iloprost-loaded platelets given i.v. during injury have also been observed. Whereas the incorporation of [3H]-thymidine into aorta intima-media DNA at 3 days post injury was 62-fold higher in balloon injured rats than in control sham operated rats, thymidine incorporation into intima/media of rats which had received iloprost loaded platelets during injury was reduced as compared with rats subjected only to the injury procedure. The reduction was only of near significance, however, but at 14 days after injury the total DNA content of the aorta intima/media of rats given iloprost loaded platelets during injury was significantly reduced. Although iloprost loaded platelets can clearly inhibit excessive platelet deposition, other encapsulated agents may have greater anti-proliferative effects.
Tárgyszavak:	Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban külföldön készült közlemény
Megjelenés:	Thrombosis And Haemostasis. - 73 : 3 (1995), p. 535-542. -
További szerzők:	Chajara, A. Pfliegler György (1949-) (belgyógyász, hematológus, labor szakorvos) El-Gamal, Basiouny Brewer, L. Capron, L.
Internet cím:	Intézményi repozitóriumban (DEA) tárolt változat
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001-es BibID:	BIBFORM034615
035-os BibID:	PMID:1280865
Első szerző:	El-Gamal, Basiouny
Cím:	Effect of platelet encapsulated Iloprost on platelet aggregation and adhesion to collagen and injured blood vessels in vitro / B. A. B. El-Gamal, G. Pfliegler, N. Crawford
Dátum:	1992
ISSN:	0340-6245
Megjegyzések:	A novel approach to site-directed delivery of drugs in vivo using blood platelets as carrier vehicles is being investigated. In this context some initial studies are reported on the effect of platelet encapsulated anti-platelet drugs on platelet aggregation and adhesion to fibrillar collagen and injured arteries in vitro. The stable prostacyclin analogue Iloprost has been encapsulated within human and pig platelets by high voltage electroporation (Hughes and Crawford 1989 and 1990). After resealing the platelets, the packaged drug has a negligible effect upon platelet adhesion to a surface of fibrillar collagen or to damaged aorta (stripped to the tunica media to simulate deep injury). The rate of platelet recruitment to the collagen shows no dose dependency with respect to intracellular Iloprost concentrations. After high Iloprost loading, as few as 2% drug loaded platelets in a mixture with control (sham encapsulated) platelets, inhibit agonist-induced platelet aggregation > 50%. The prior deposition of a "lawn" of Iloprost-loaded platelets onto fibrillar collagen or damaged aorta has a substantial inhibitory effect (50-70%) upon the secondary recruitment of normal platelets compared with recruitment to a "lawn" of normal platelets. This inhibition of secondary recruitment occurs even in the presence of a platelet activator. If reduction of platelet recruitment to a vessel wall lesion results in a decrease in the local concentration of platelet granule-derived smooth muscle cell chemotactic and proliferative factors, this site-directed drug delivery may well have application for the prevention of restenosis following balloon angioplasty procedures.
Tárgyszavak:	Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban külföldön készült közlemény
Megjelenés:	Thrombosis And Haemostasis. - 68 : 5 (1992), p. 606-614. -
További szerzők:	Pfliegler György (1949-) (belgyógyász, hematológus, labor szakorvos) Crawford, Neville
Internet cím:	Intézményi repozitóriumban (DEA) tárolt változat
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001-es BibID:	BIBFORM034507
Első szerző:	Pfliegler György (belgyógyász, hematológus, labor szakorvos)
Cím:	Thrombocytákba zárt prostacyclin (iloprost) hatása az adhézióra és az aggregációra kollagénen és sérült aortán in vitro, valamint angioplasztikát követően in vivo / Pfliegler György, Bas El-Gamal, Juan José Badimon, Neville Crawford
Dátum:	1994
Tárgyszavak:	Orvostudományok Klinikai orvostudományok magyar nyelvű folyóiratközlemény hazai lapban egyetemen (Magyarországon) készült közlemény
Megjelenés:	Magyar Belorvosi Archivum. - 47 : 6 (1994), p. 453-459. -
További szerzők:	El-Gamal, Basiouny Badimon, Juan José Crawford, Neville
Internet cím:	Intézményi repozitóriumban (DEA) tárolt változat
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001-es BibID:	BIBFORM034508
Első szerző:	Pfliegler György (belgyógyász, hematológus, labor szakorvos)
Cím:	Inhibition of platelet recruitment to arterial lesions by predeposition of platelets containing encapsulated iloprost / George Pfliegler, Basiouny El-Gamal, Juan José Badimon, Lina Badimon, Neville Crawford
Dátum:	1994
ISSN:	0340-6245
Megjegyzések:	Drugs can be encapsulated within blood platelets by reversible electroporation and can be haemostatically targeted to vessel wall injury sites. Initial studies with iloprost-loaded pig platelets and pig aorta tunica media in perfusion circuits are presented. After autologous reconstitution into blood, no significant difference was observed in the deposition of 111Indium labelled sham-loaded and untreated platelets onto the tunica media during perfusion under low and high shear conditions. In paired experiments (n = 10 pairs), the deposition of iloprost-loaded platelets was significantly lower (mean 61%) after 5 min perfusion than the deposition from blood containing sham-loaded (control) platelets. A similar significant reduction (mean 54%) was seen after 10 min perfusion. Pre-perfusion of iloprost-loaded platelets for 10 min under low shear conditions (212/s), followed by 5 min perfusion of 111Indium labelled normal platelets, significantly reduced the secondary platelet deposition (p < 0.01) when compared with the deposition seen when control untreated platelets were preperfused. Significant differences (p < 0.001) in secondary deposition were also observed when primary and secondary platelet perfusions were made under high shear (1690/s). Histology of the tunica media segments post perfusion, supported the inhibitory effect of predeposited iloprost-loaded platelets on secondary platelet recruitment. By exploiting their natural haemostatic propensity, drug-loaded platelets can be targeted to vessel wall injury sites. Appropriate drugs could be packaged that may passivate the carrier platelets at the lesion inhibiting thrombus formation or they may act as a depot for sustained drug release.(
Tárgyszavak:	Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban külföldön készült közlemény
Megjelenés:	Thrombosis And Haemostasis. - 72 : 4 (1994), p. 604-610. -
További szerzők:	El-Gamal, Basiouny Badimon, Juan José Badimon, Lina Crawford, Neville
Internet cím:	Intézményi repozitóriumban (DEA) tárolt változat
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