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001-es BibID:	BIBFORM033271
035-os BibID:	WOS:000168668400003
Első szerző:	Ádám Zsuzsa
Cím:	Liver metastatic ability of human melanoma cell line is associated with losses of chromosomes 4, 9p21-pter and 10p / Zsuzsanna Ádám, Róza Ádány, Andrea Ladányi, József Timár, Margit Balázs
Dátum:	2000
Megjegyzések:	Genetic changes underlying the aggressive progression of human cutaneous melanoma are not completely understood. In order to characterise genetic alterations associated with the metastatic behaviour of this neoplasm we used comparative genomic hybridisation (CGH) in combination with fluorescence in situ hybridisation (FISH) on an experimental metastatic model of three related human melanoma cell lines. Tumour lines were selected based on their various metastatic capacity to liver in immunosuppressed mice. The parental cell line (A2058) was a human amelanotic melanoma cell line, adaptation of this line to in vivo growth as xenograft the HT168 tumour and its cell line was established. After intrasplenic transplantation of HT168 cells into immunosuppressed mice, a highly metastatic variant (HT168-M1) was selected. Several chromosomal aberrations common to all three lines indicating common clonal origin, as well as additional non-shared chromosomal changes were found. The original cell line (A2058) exhibited the highest number of genetic changes. Chromosomal alterations present only in the highly metastatic line (HT168-M1) involved losses on chromosome 4, 9p21.3-pter and 10p. Chromosome copy number patterns and the nature of chromosome 4 loss were further investigated by FISH using different centromeric probes and a chromosome 4 painting probe. According to our CGH and FISH results we assume that alterations present only in the aggressive metastatic subline are associated with the increased - metastatic potential. Our observations further support the hypothesis, based on some recently published data, that certain (so far unidentified) suppressor genes having an important role in tumour progression are located on these chromosomes.
Tárgyszavak:	Orvostudományok Egészségtudományok idegen nyelvű folyóiratközlemény külföldi lapban experimental model FISH and CGH genetic alterations melanoma metastasis egyetemen (Magyarországon) készült közlemény
Megjelenés:	Clinical & experimental metastasis. - 18 : 4 (2000), p. 295-302. -
További szerzők:	Ladányi Andrea Timár József Ádány Róza (1952-) (megelőző orvostan és népegészségtan szakorvos) Balázs Margit (1952-) (sejtbiológus, molekuláris genetikus)
Internet cím:	DOI Intézményi repozitóriumban (DEA) tárolt változat
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