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001-es BibID:	BIBFORM036483
Első szerző:	Muszbek László (haematológus, kutató orvos)
Cím:	Monocytes of patients congenitally deficient in plasma factor XIII lack factor XIII subunit A antigen and transglutaminase activity / Muszbek L., Adány R., Kávai M., Boda Z., Lopaciuk S.
Dátum:	1988
ISSN:	0340-6245
Megjegyzések:	Monocytes isolated from patients with severe deficiency in plasma Factor XIII of blood coagulation (FXIII) were tested for FXIII antigen and transglutaminase activity. By immunoperoxidase method the patients' monocytes, in contrast to normal controls, showed no reaction with a monospecific antibody against FXIII subunit a. This result was confirmed by immunoblotting technique, as well. In addition, tissue macrophages tested in one of the patients were also exempt of FXIII subunit a antigen. The transglutaminase activity in FXIII deficient monocytes was below the limit of the detection of the dansylcadaverine incorporation assay. The results suggest that FXIII subunit a of monocytes/macrophages and its plasma and platelet counterparts are closely related or identical proteins and demonstrate that the transglutaminase activity in monocytes is of FXIII origin and tissue transglutaminase is present, if at all, only in insignificant amount.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban factor XIII monocytes transglutaminase
Megjelenés:	Thrombosis And Haemostasis. - 59 : 2 (1988), p. 231-235. -
További szerzők:	Ádány Róza (1952-) (megelőző orvostan és népegészségtan szakorvos) Kávai Mária (1930-) (vegyész) Boda Zoltán (1947-) (belgyógyász, haematologus, klinikai onkológus) Lopaciuk, Stanislaw
Internet cím:	Intézményi repozitóriumban (DEA) tárolt változat
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001-es BibID:	BIBFORM006790
Első szerző:	Sárváry Attila (népegészségtan szakorvos)
Cím:	Possible role of factor XIII subunit A in Fcgamma and complement receptor-mediated phagocytosis / Sarvary, A., Szucs, S., Balogh, I., Becsky, A., Bardos, H., Kavai, M., Seligsohn, U., Egbring, R., Lopaciuk, S., Muszbek, L., Adany, R.
Dátum:	2004
ISSN:	0008-8749
Megjegyzések:	Besides its traditional role in hemostasis, factor XIII subunit A (FXIII-A) is supposed to function as a cellular transglutaminase and to be involved in certain intracellular processes, including cytoskeletal remodeling. To investigate its intracellular role, the aim of the present study was to follow changes in FXIII-A production in combination with the receptor-mediated phagocytic activities of monocytes/macrophages and to examine the phagocytic functions of monocytes in patients with FXIII-A deficiency. Human blood monocytes were isolated from the buffy coats of healthy volunteers and cultured for 4 days. The FcgammaR-mediated phagocytosis of sensitized erythrocytes (EA) and the complement receptor (CR)-mediated phagocytosis of complement-coated yeast particles were studied during monocyte/macrophage differentiation. Changes in the gene expression of FXIII-A were detected by real-time quantitative RT-PCR. FXIII-A protein production was investigated with fluorescent image analysis at single cell level and Western immunoblot analysis. Both the FcgammaR and CR-mediated phagocytosis increased during culturing, which peaked on day 3. The phagocytic activity of the cells could be markedly inhibited with monodansylcadaverine, an inhibitor of the transglutaminase-induced crosslinking of proteins. The phagocytosis of EA, complement-coated and uncoated yeast particles was found to be strongly diminished in monocytes of FXIII-A deficient patients. The phagocytic functions of cultured cells showed a change in parallel with the alterations in FXIII-A mRNA expression, as well as with that in FXIII-A in protein synthesis detected by image and Western immunoblot analyses in concert. Our results suggest that FXIII-A plays a role in the Fcgamma and complement receptor-mediated phagocytic activities of monocytes/macrophages.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban Blotting, Western Cadaverine Enzyme Inhibitors Erythrocytes Factor XIII Female Humans Macrophages Male Microscopy, Fluorescence Phagocytosis RNA Receptors, Complement 3b Receptors, IgG Reverse Transcriptase Polymerase Chain Reaction Transglutaminases
Megjelenés:	Cellular Immunology. - 228 : 2 (2004), p. 81-90. -
További szerzők:	Szűcs Sándor (1958-) (biokémikus, vegyész) Balogh Imre Becsky Áron Bárdos Helga (1969-) (megelőző orvostan és népegészségtan szakorvos) Kávai Mária (1930-) (vegyész) Seligsohn, Uri Egbring, Rudolf Lopaciuk, Stanislaw Muszbek László (1942-) (haematológus, kutató orvos) Ádány Róza (1952-) (megelőző orvostan és népegészségtan szakorvos)
Internet cím:	elektronikus változat DOI
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