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1.

001-es BibID:BIBFORM046289
Első szerző:Bacsó Zsolt (biofizikus)
Cím:A photobleaching energy transfer analysis of CD8MHC-I and LFA-1ICAM-1 interactions in CTL-target cell conjugates / Bacsó Zsolt, Bene László, Bodnár Andrea, Matkó János, Damjanovich Sándor
Dátum:1996
ISSN:0165-2478
Megjegyzések:The photobleaching energy transfer (pbFRET) technique is a fluorescence method to measure proximity relationships between molecules, especially cell surface proteins, labeled with fluorophore-conjugated monoclonal antibodies, on a pixel-by-pixel base using digital imaging microscopy. This technique enables analysis of inter- and intramolecular proximities at cell surfaces at physiological conditions. We have developed a pbFRET approach to measure intercellular proximities in order to access spatial organization of interacting proteins in the contact region of two 'communicating' cells. Two examples, as possible application areas of this approach, are presented here: interaction between CD8 and MHC-I molecules in point contacts and interaction between LFA-1 and ICAM-1 molecules in focal contacts of CTL-target conjugates. The geometry of these protein contacts based on our resonance energy transfer (RET) data is consistent with the observed blocking effects of monoclonal antibodies (directed against the interacting proteins) on the cytolytic activity of CTLs and suggest a critical role for CD8beta-subunit in signal transmission in peripheral T-lymphocytes
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:Immunology Letters. - 54 : 2-3 (1996), p. 151-156. -
További szerzők:Bene László (1963-) (biofizikus) Dóczy-Bodnár Andrea (1970-) (biofizikus) Matkó János (1952-) (biológus) Damjanovich Sándor (1936-2017) (biofizikus)
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DOI
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2.

001-es BibID:BIBFORM046291
Első szerző:Dóczy-Bodnár Andrea (biofizikus)
Cím:Modification of membrane cholesterol level affects expression and clustering of class I HLA molecules at the surface of JY human lymphoblasts / Bodnár Andrea, Jenei Attila, Bene László, Damjanovich Sándor, Matkó János
Dátum:1996
ISSN:0165-2478
Megjegyzések:Recently we have found that class I HLA molecules, key elements of the antigen presentation system for CD8 + effector cells, show a clustered lateral distribution (homoassociation) at the surface of activated human T- and B-lymphocytes as well as virus-transformed T- and B-lymphoblasts, in contrast to a disperse distribution on resting human PBLs (Matk6 et al. (1994) J. Immunol. 152, 3353; Bene et al. (1994) Eur. J. Immunol. 24, 2115). Expression of beta2m-free HLA heavy chains and exogenous beta2m have been shown as potential regulation factors of HLA-I clustering, which in turn may affect cytotoxic activity of CD8+ effector cells. Here we report a study on the effect of plasma membrane-modification (by exogenous cholesterol and phosphatidylcholine) on the expression of free HLA heavy chains and beta2m-bound HLA-I molecules on JY human B-lymphoblasts. The modulating effect of these two treatments on the lipid fluidity of cells was demonstrated by fluorescence anisotropy of DPH lipid probe. The lateral clustering (association) of HLA-I molecules was detected by flow cytometric fluorescence resonance energy transfer (FCET) and digital imaging microscopic photobleaching energy transfer (pbFRET) methods, using flourescein-isothiocyanate (FITC) (donor)- and tetramethyl-rhodamine-isothiocyanate (TRITC) (acceptor)-labeled W6/32 or KE2 antibodies directed against intact HLA-I molecules. Cholesterol enrichment of the plasma membrane increased membrane fluidity and reduced the expression of heavy- and light-chain determinants of HLA-I molecules and free heavy chains (FHCs). This was accompanied with a higher degree of HLA-I clustering as shown by the enhanced intermolecular energy transfer efficiency. In contrast, cholesterol depletion resulted in membrane fluidization and increased expression of HLA-I epitopes. Our results suggest that both cholesterol level and lipid structure/fluidity of the plasma membrane in lymphoblastoid cells may also potentially regulate lateral organization and consequently the presentation efficiency of HLA-I molecules.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:Immunology Letters. - 54 : 2-3 (1996), p. 221-226. -
További szerzők:Jenei Attila (1966-) (biofizikus) Bene László (1963-) (biofizikus) Damjanovich Sándor (1936-2017) (biofizikus) Matkó János (1952-) (biológus)
Pályázati támogatás:T6163
OTKA
6221
OTKA
17592
OTKA
F020102
OTKA
Internet cím:Szerző által megadott URL
DOI
Intézményi repozitóriumban (DEA) tárolt változat
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3.

001-es BibID:BIBFORM005175
Első szerző:Dóczy-Bodnár Andrea (biofizikus)
Cím:A biophysical approach to IL-2 and IL-15 receptor function : localization, conformation and interactions / Bodnar, A., Nizsaloczki, E., Mocsar, G., Szaloki, N., Waldmann, T. A., Damjanovich, S., Vamosi, G.
Dátum:2008
Megjegyzések:Interleukin-2 and interleukin-15 (IL-2, IL-15) are key participants in T and NK cell activation and function. Sharing the beta and gamma receptor subunits results in several common functions: e.g. the promotion of T cell proliferation. On the other hand, due to their distinct alpha receptor subunits, they also play opposing roles in immune processes such as activation induced cell death and immunological memory. Divergence of signaling pathways must ensue already at the plasma membrane where the cytokines interact with their receptors. Therefore understanding molecular details of receptor organization and mapping interactions with other membrane proteins that might influence receptor conformation and function, are of key importance. Biophysical/advanced microscopic methods (fluorescence resonance energy transfer (FRET), fluorescence crosscorrelation spectroscopy (FCCS), near-field scanning optical microscopy (NSOM), X-ray crystallography, surface plasmon resonance, NMR spectroscopy) have been instrumental in clarifying the details of receptor structure and organization from the atomic level to the assembly and dynamics of supramolecular clusters. In this short review some important contributions shaping our current view of IL-2 and IL-15 receptors are presented.
Tárgyszavak:Orvostudományok Elméleti orvostudományok Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
analysis
Antibodies
Apoptosis
Biophysics
blood
Carcinoma
Cell Differentiation
Cell Fusion
Cell Line
Cell Membrane
Cell Proliferation
Cells
Complement
Confocal microscopy
Dendritic Cells
Diffusion
Energy Transfer
Epidermal Growth Factor
Fibroblasts
Fluorescence
Fluorescence correlation spectroscopy
Fluorescence Resonance Energy Transfer
FRET
Homeostasis
Human
Hungary
IL-2 and IL-15 receptors
In Vitro
Insulin
Interleukin-15
Interleukin-2
Kinetics
Lymphocytes
Lymphoma
Melanoma
Membrane Microdomains
Membrane Proteins
metabolism
methods
Mice
Microscopy
Near-field scanning optical microscopy
Phagocytosis
Phosphorylation
Photobleaching
Protein-protein interactions
Proteins
Receptor patterns
Research
Signal Transduction
Structure determination
Support
therapy
transmembrane signaling
Tyrosine
X-ray crystallography
Megjelenés:Immunology Letters. - 116 : 2 (2008), p. 117-125. -
További szerzők:Nizsalóczki Enikő Mocsár Gábor (1981-) (biofizikus) Szalóki Nikoletta (1981-) (biológus) Waldmann, Thomas A. Damjanovich Sándor (1936-2017) (biofizikus) Vámosi György (1967-) (biofizikus)
Internet cím:elektronikus változat
elektronikus változat
DOI
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4.

001-es BibID:BIBFORM005107
Első szerző:Vámosi György (biofizikus)
Cím:The role of supramolecular protein complexes and membrane potential in transmembrane signaling processes of lymphocytes / Vamosi, G., Bodnar, A., Damjanovich, S., Nagy, P., Varga, Z., Damjanovich, L.
Dátum:2006
ISSN:165-2478 (Print)
Megjegyzések:The formation of protein patterns in lymphocyte plasma membranes is analyzed in the light of past and, also, very recent experiments. The analysis surveys the lateral organization of major histocompatibility complex glycoproteins, intercellular adhesion molecule-1, interleukin-2 and -15 receptors, Kv1.3 K+ ion channels and the T-cell receptor as well as their behavior under different conditions. These molecules form small- and large-scale clusters in the membrane of human lymphocytes. Many of the association motifs occur in other investigated cell types. The conclusions point toward a possible role for ion channel activities, membrane potential changes and alterations of the lateral organization of proteins in transmembrane signaling and cytotoxic interactions. In our outlook new factors that potentially affect membrane protein cluster formation and interactions are discussed. A role for MHC glycoproteins in concentrating membrane proteins and organizing protein patterns is suggested, and the possibility that the membrane potential may modulate protein conformation and, thereby, affect protein-protein interactions is pointed out. A well-defined role for the presence of ion channels in the immune synapse is offered, which could explain the significance of ion channel accumulation in the immune synapse together with the T-cell receptor.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
analysis
Animals
Biophysics
Cell Membrane
Glycoproteins
Human
Humans
Hungary
immunology
Intercellular Adhesion Molecule-1
Interleukin-2
Ion Channels
Light
Lymphocytes
Major Histocompatibility Complex
Membrane Potentials
Membrane Proteins
metabolism
Multiprotein Complexes
physiology
Protein Conformation
Proteins
Research
Signal Transduction
Support
T-Lymphocytes
Megjelenés:Immunology Letters. - 104 : 1-2 (2006), p. 53-58. -
További szerzők:Dóczy-Bodnár Andrea (1970-) (biofizikus) Damjanovich Sándor (1936-2017) (biofizikus) Nagy Péter (1971-) (biofizikus) Varga Zoltán (1969-) (biofizikus, szakfordító) Damjanovich László (1960-) (általános sebész)
Internet cím:DOI
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