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001-es BibID:BIBFORM005175
Első szerző:Dóczy-Bodnár Andrea (biofizikus)
Cím:A biophysical approach to IL-2 and IL-15 receptor function : localization, conformation and interactions / Bodnar, A., Nizsaloczki, E., Mocsar, G., Szaloki, N., Waldmann, T. A., Damjanovich, S., Vamosi, G.
Dátum:2008
Megjegyzések:Interleukin-2 and interleukin-15 (IL-2, IL-15) are key participants in T and NK cell activation and function. Sharing the beta and gamma receptor subunits results in several common functions: e.g. the promotion of T cell proliferation. On the other hand, due to their distinct alpha receptor subunits, they also play opposing roles in immune processes such as activation induced cell death and immunological memory. Divergence of signaling pathways must ensue already at the plasma membrane where the cytokines interact with their receptors. Therefore understanding molecular details of receptor organization and mapping interactions with other membrane proteins that might influence receptor conformation and function, are of key importance. Biophysical/advanced microscopic methods (fluorescence resonance energy transfer (FRET), fluorescence crosscorrelation spectroscopy (FCCS), near-field scanning optical microscopy (NSOM), X-ray crystallography, surface plasmon resonance, NMR spectroscopy) have been instrumental in clarifying the details of receptor structure and organization from the atomic level to the assembly and dynamics of supramolecular clusters. In this short review some important contributions shaping our current view of IL-2 and IL-15 receptors are presented.
Tárgyszavak:Orvostudományok Elméleti orvostudományok Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
analysis
Antibodies
Apoptosis
Biophysics
blood
Carcinoma
Cell Differentiation
Cell Fusion
Cell Line
Cell Membrane
Cell Proliferation
Cells
Complement
Confocal microscopy
Dendritic Cells
Diffusion
Energy Transfer
Epidermal Growth Factor
Fibroblasts
Fluorescence
Fluorescence correlation spectroscopy
Fluorescence Resonance Energy Transfer
FRET
Homeostasis
Human
Hungary
IL-2 and IL-15 receptors
In Vitro
Insulin
Interleukin-15
Interleukin-2
Kinetics
Lymphocytes
Lymphoma
Melanoma
Membrane Microdomains
Membrane Proteins
metabolism
methods
Mice
Microscopy
Near-field scanning optical microscopy
Phagocytosis
Phosphorylation
Photobleaching
Protein-protein interactions
Proteins
Receptor patterns
Research
Signal Transduction
Structure determination
Support
therapy
transmembrane signaling
Tyrosine
X-ray crystallography
Megjelenés:Immunology Letters. - 116 : 2 (2008), p. 117-125. -
További szerzők:Nizsalóczki Enikő Mocsár Gábor (1981-) (biofizikus) Szalóki Nikoletta (1981-) (biológus) Waldmann, Thomas A. Damjanovich Sándor (1936-2017) (biofizikus) Vámosi György (1967-) (biofizikus)
Internet cím:elektronikus változat
elektronikus változat
DOI
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2.

001-es BibID:BIBFORM097487
Első szerző:Kenesei Ádám
Cím:IL-15 Trans-Presentation Is an Autonomous, Antigen-Independent Process / Kenesei Ádám, Volkó Julianna, Szalóki Nikoletta, Mocsár Gábor, Jambrovics Károly, Balajthy Zoltán, Bodnár Andrea, Tóth Katalin, Waldmann Thomas A., Vámosi György
Dátum:2021
ISSN:0022-1767 1550-6606
Megjegyzések:IL-15 plays a pivotal role in the long-term survival of T cells and immunological memory. Its receptor consists of three subunits (IL-15R?, IL-2/15R?, and ?c). IL-15 functions mainly via trans-presentation (TP), during which an APC expressing IL-15 bound to IL-15R? presents the ligand to the ??c receptor-heterodimer on a neighboring T/NK cell. To date, no direct biophysical evidence for the intercellular assembly of the IL-15R heterotrimer exists. Ag presentation (AP), the initial step of T cell activation, is also based on APC?T cell interaction. We were compelled to ask whether AP has any effect on IL-15 TP or whether they are independent processes. In our human Raji B cell?Jurkat T cell model system, we monitored inter-/intracellular protein interactions upon formation of IL-15 TP and AP receptor complexes by Förster resonance energy transfer measurements. We detected enrichment of IL-15R? and IL-2/15R? at the synapse and positive Förster resonance energy transfer efficiency if Raji cells were pretreated with IL-15, giving direct biophysical evidence for IL-15 TP. IL-15R? and MHC class II interacted and translocated jointly to the immunological synapse when either ligand was present, whereas IL-2/15R? and CD3 moved independently of each other. IL-15 TP initiated STAT5 phosphorylation in Jurkat cells, which was not further enhanced by AP. Conversely, IL-15 treatment slightly attenuated Ag-induced phosphorylation of the CD3? chain. Our studies prove that in our model system, IL-15 TP and AP can occur independently, and although AP enhances IL-15R assembly, it has no significant effect on IL-15 signaling during TP. Thus, IL-15 TP can be considered an autonomous, Ag-independent process.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
interleukin
trans-presentation
immunology
Megjelenés:Journal Of Immunology. - 207 : 10 (2021), p. 2489-2500. -
További szerzők:Volkó Julianna (1983-) (biotechnológus) Szalóki Nikoletta (1981-) (biológus) Mocsár Gábor (1981-) (biofizikus) Jambrovics Károly (1988-) (biológus, gyógyszer-biotechnológus) Balajthy Zoltán (1957-) (biokémikus, sejtbiológus) Dóczy-Bodnár Andrea (1970-) (biofizikus) Tóth Katalin Ágnes (1977-) (biokémikus, molekuláris biológus) Waldmann, Thomas A. Vámosi György (1967-) (biofizikus)
Pályázati támogatás:GINOP-2.3.2-15-2016-00026
GINOP
EFOP-3.6.3-VEKOP-16-2017-00009
EFOP
Tempus Public Foundation 273478
Egyéb
Deutscher Akademischer Austauschdienst 273478
Egyéb
HHS | NIH | National Cancer Institute intramural research program
Egyéb
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DOI
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