Találati lista | Tudóstér

001-es BibID:	BIBFORM006030
Első szerző:	Damjanovich Sándor (biofizikus)
Cím:	Ion channel activity and transmembrane signaling in lymphocytes / Damjanovich S., Pieri C., Trón L.
Dátum:	1992
Megjegyzések:	Transmembrane signalling refers to the process of transfer of information from the extracellular world into the intracellular space. The information is transduced through several possible pathways. The significance of cell surface dynamics, ion channel activity and drug effects are discussed in the signal transmission, with special reference to Na+ channels and the Ca2+ sensitive potassium channels.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban Animal Bretylium Tosylate Cell Membrane Cyclosporine drug effects Human Hungary Ion Channel Gating Ion Channels Lymphocyte Transformation Lymphocytes Membrane Potentials pharmacology physiology Potassium Potassium Channels Second Messenger Systems Signal Transduction Support,Non-U.S.Gov't
Megjelenés:	Annals of the New York Academy of Sciences. - 650 (1992), p. 205-210. -
További szerzők:	Pieri, Carlo Trón Lajos (1941-) (biofizikus)
Internet cím:	elektronikus változat
Borító:
Saját polcon:

001-es BibID:	BIBFORM006029
Első szerző:	Damjanovich Sándor (biofizikus)
Cím:	Electroimmunology : membrane potential, ion-channel activities, and stimulatory signal transduction in human T lymphocytes from young and elderly / Damjanovich S., Pieri C.
Dátum:	1991
Megjegyzések:	There are conflicting data on the functional role and direction of the changes in membrane potential and ion currents accompanying lymphocyte stimulation. Recently, we discovered that a known sodium channel opener, bretylium tosylate (BT), may influence the stimulatory processes of lymphocyte activation at more than one site. Parallel flow cytometric and electrophysiological measurements with patch clamp techniques showed that BT quickly repolarizes previously slightly depolarized human peripheral blood as well as splenic murine lymphocytes. The repolarization occurred through opening ligand- and voltage-gated, hitherto unknown sodium channels, and the sodium influx activated Na(+)-K(+)-dependent, electrogenic ATP-ase activity. A comparison of the flexible responsiveness of the membrane potential was carried out between lymphocytes from young and elderly using the above mechanism and a number of combinations of channel blockers and ionophores in order to obtain information on the alleged changes in immunological behavior. A significant difference has been found between lymphocytes from human young and elderly volunteers in the readiness to respond to channel-activating perturbations. An explanation is offered, based upon known physicochemical changes in the plasma membrane during aging.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban Adult Age Factors Aged Aging blood Bretylium Tosylate Cell Membrane Human Hungary immunology Ion Channels Ionophores Lymphocytes Membrane Potentials physiology Signal Transduction Sodium Sodium Channels T-Lymphocytes
Megjelenés:	Annals of the New York Academy of Sciences. - 621 (1991), p. 29-39. -
További szerzők:	Pieri, Carlo
Internet cím:	elektronikus változat
Borító:
Saját polcon:

001-es BibID:	BIBFORM006051
Első szerző:	Pieri, Carlo
Cím:	The response of human lymphocytes to phytohemagglutinin is impaired at different levels during aging / Pieri, C., Recchioni, R., Moroni, F., Marcheselli, F., Damjanovich, S.
Dátum:	1992
Megjegyzések:	Several parameters generally believed to be necessary for the activation and progression of proliferation of human lymphocytes have been investigated and compared with special reference to aging. The responding capacity of plasma membrane potential to depolarizing and also repolarizing conditions induced by exposure to mitogens like PHA was lower in lymphocytes from old donors as compared to those of young ones. This indicates a significant age-dependent difference in the readiness to respond to channel-activating perturbations. As an early signal of activation, after one hour PHA stimulation the merocyanine 540 uptake by the lipid regions was chosen, based on the property of this fluorescent probe to bind to loosely packed lipids of the plasma membrane. The proteins encoded by the c-myc and c-myb genes were chosen as markers of the G0/G1 and G1/S phased transition, respectively. The mean number of cells that increased the uptake of MC 540 following mitogenic stimulation did not differ in young vs. old individuals. However, 4 samples out of 10 from the old population showed lower MC 540 fluorescence than the lowest signal from the young population. The number of responding cells was decreased during aging when the presence of the c-myc protein was taken as its measure; and this decrease was further accentuated, determining the expression of the c-myb protein. This frequently encountered age-dependent pattern, however, was not followed by the lymphocytes of all old donors. One example is reported in which the MC 540 uptake, the c-myc and c-myb expression in the cells from one old subject fell in the range of the young subjects. However, even in this case, the response of the lymphocytes as measured by 3H-thymidine incorporation was only 64% of that of young subjects. For this sample, we found an impairment of the response at the mitochondrial level. In addition to these parameters, the amount of 3H-thymidine incorporated by the cells expressing the c-myb protein was calculated. The values in old individuals were lower than those in the young, suggesting that not all the cells expressing the c-myb protein were able to synthesize DNA in lymphocyte populations from the elderly. Our data support the view that the age-dependent decline of lymphocyte responsiveness to mitogens can be accounted for by impairments at different levels.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban Aging Cell Membrane Dna DNA-Binding Proteins drug effects Fluorescence Genome Human Lymphocytes Membrane Potentials metabolism pharmacology physiology Phytohemagglutinins Proto-Oncogene Proteins Proto-Oncogene Proteins c-myb Proto-Oncogene Proteins c-myc Research Support,Non-U.S.Gov't Thymidine
Megjelenés:	Annals of the New York Academy of Sciences. - 673 (1992), p. 110-119. -
További szerzők:	Recchioni, Rina Moroni, Fausto Marcheselli, Fiorella Damjanovich Sándor (1936-2017) (biofizikus)
Internet cím:	elektronikus változat
Borító:
Saját polcon: