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1.

001-es BibID:BIBFORM004699
Első szerző:Bacsó Zsolt (biofizikus)
Cím:INF-gamma rearranges membrane topography of MHC-I and ICAM-1 in colon carcinoma cells / Bacso, Z., Bene, L., Damjanovich, L., Damjanovich, S.
Dátum:2002
Megjegyzések:Flow-cytometric fluorescence energy transfer (FCET) measurements between fluorescently labeled cell surface MHC-I and ICAM-1 molecules indicated similar receptor patterns in the plasma membrane of interferon-gamma (INF-gamma)-treated colon carcinoma cells as those observed earlier at the surface of lymphoid cells. INF-gamma activation significantly increased the density of MHC-I and ICAM-1 proteins in the membrane. This increase in receptor density was accompanied by decreased proximity level of the homo-associated MHC-I receptors. Hetero-association of MHC-I and ICAM-1 molecules was increased by INF-gamma treatment. INF-gamma changed neither hetero- nor homo-association of transferrin receptors. By staining the sphingomyelin/cholesterol-enriched lipid microdomains with fluorescently labeled cholera toxin B subunit, we found an increase in the amount of lipid-raft associated G(M1)-gangliosides due to INF-gamma treatment. Confocal microscopic results and FCET measurements show that MHC-I and ICAM-1 are components of G(M1)-ganglioside containing lipid-rafts and also support an increase in the size of these lipid-rafts upon INF-gamma treatment.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Carcinoma
Cell Membrane
chemistry
Cholera Toxin
Colonic Neoplasms
drug effects
Energy Transfer
Flow Cytometry
Fluorescein-5-isothiocyanate
Fluorescence
G(M1) Ganglioside
Histocompatibility Antigens Class I
Human
Hungary
Intercellular Adhesion Molecule-1
Interferon Type II
Membrane Microdomains
metabolism
Microscopy,Confocal
pathology
pharmacology
Protein Binding
Receptors,Cell Surface
Receptors,Transferrin
Support,Non-U.S.Gov't
Tumor Cells,Cultured
Megjelenés:Biochemical and Biophysical Research Communications. - 290 : 2 (2002), p. 635-640. -
További szerzők:Bene László (1963-) (biofizikus) Damjanovich László (1960-) (általános sebész) Damjanovich Sándor (1936-2017) (biofizikus)
Internet cím:DOI
elektronikus változat
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2.

001-es BibID:BIBFORM005965
Első szerző:Balázs Margit (sejtbiológus, molekuláris genetikus)
Cím:Accessibility of cell surface thiols in human lymphocytes is altered by ionophores or OKT-3 antibody / Margit Balázs, János Matkó, János Szöllösi, László Mátyus, Mack J. Fulwyler, Sándor Damjanovich
Dátum:1986
Megjegyzések:The accessibility of cell surface sulfhydryl groups in human peripheral lymphocytes was investigated with 5,5'-dithiobis-(2-nitrobenzoic acid) in the presence and absence of ionophore antibiotics and the monoclonal antibody, OKT-3. Only a few accessible protein thiols have been found on the cells as demonstrated by labeling with a fluorescent non-penetrating thiol-marker, monobromotrimethyl-ammoniobimane and the subsequent gel electrophoretic analysis of the protein pattern. Difference spectrophotometric measurement of thiol-DTNB reaction revealed that ionophores altering the transmembrane potential induced an enhanced cell surface thiol-exposure on the minute time scale. The effect showed a dependence on the external concentration of the cations. The binding of monoclonal antibody, OKT-3, directed against T3 complexes, resulted in a similar, concentration-dependent increase of thiol-accessibility. These data are interpreted as early membrane-effects of ionophores and the specific antibody including changes in the conformational equilibrium or vertical displacements of certain membrane proteins. These events are likely to be coupled to the changes in the transmembrane potential of the lymphocytes.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Ammonium Compounds
analysis
Antibiotics
Antibodies,Monoclonal
blood
Cell Membrane
Dithionitrobenzoic Acid
drug effects
Electrophoresis,Polyacrylamide Gel
Human
Ionophores
Lymphocytes
Membrane Proteins
metabolism
pharmacology
Spectrometry,Fluorescence
Sulfhydryl Compounds
Support,Non-U.S.Gov't
Support,U.S.Gov't,P.H.S.
Megjelenés:Biochemical and Biophysical Research Communications. - 140 : 3 (1986), p. 999-1006. -
További szerzők:Matkó János (1952-) (biológus) Szöllősi János (1953-) (biofizikus) Mátyus László (1956-) (biofizikus) Fulwyler, Mack J. Damjanovich Sándor (1936-2017) (biofizikus)
Internet cím:elektronikus változat
Intézményi repozitóriumban (DEA) tárolt változa
DOI
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3.

001-es BibID:BIBFORM004830
035-os BibID:(Scopus)14644442912 (WoS)000223675800046
Első szerző:Bene László (biofizikus)
Cím:Membrane topography of HLA I, HLA II, and ICAM-1 is affected by IFN-gamma in lipid rafts of uveal melanomas / László Bene, Andrea Bodnár, Sándor Damjanovich, György Vámosi, Zsolt Bacsó, János Aradi, András Berta, Judit Damjanovich
Dátum:2004
Megjegyzések:The lateral distribution and colocalization of HLA I, HLA-DR, and ICAM-1 proteins was studied for the first time in the plasma membrane of two human uveal melanoma cell lines, OCM-1 and OCM-3. Our fluorescence resonance energy transfer and confocal laser scanning microscopic experiments revealed that these molecules are mostly confined to the same membrane regions, where they form similar protein patterns (homo- and hetero-associates) to those found previously on other cell types of lymphoid as well as colorectal carcinoma origin. Confocal microscopic colocalization experiments with GM(1) gangliosides and the GPI-anchored CD59 molecules showed enrichment of HLA I, HLA-DR, and ICAM-1 molecules in specific membrane domains (lipid rafts) excluding the transferrin receptor. IFN-gamma remarkably increased the expression levels of these molecules and rearranged their association patterns, which can affect the adoptive immune response of effector cells
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Antigens
Biophysics
Carcinoma
Cell Line
Cells
Energy Transfer
Flow Cytometry
Fluorescence
Fluorescence Resonance Energy Transfer
Histocompatibility Antigens
Histocompatibility Antigens Class I
Histocompatibility Antigens Class II
Human
Humans
Hungary
Intercellular Adhesion Molecule-1
Interferon Type II
Melanoma
Membrane Microdomains
metabolism
Microscopy,Confocal
Proteins
Research
Support
Uveal Neoplasms
egyetemen (Magyarországon) készült közlemény
Megjelenés:Biochemical and Biophysical Research Communications. - 322 : 2 (2004), p. 678-683. -
További szerzők:Dóczy-Bodnár Andrea (1970-) (biofizikus) Damjanovich Sándor (1936-2017) (biofizikus) Vámosi György (1967-) (biofizikus) Bacsó Zsolt (1963-) (biofizikus) Aradi János (1942-) (biokémikus, vegyész) Berta András (1955-) (szemész, gyermekszemész) Damjanovich Judit (1963-) (szemész)
Internet cím:DOI
Intézményi repozitóriumban (DEA) tárolt változat
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4.

001-es BibID:BIBFORM005934
Első szerző:Damjanovich Sándor (biofizikus)
Cím:The number of SH groups in rabbit muscle phosphorylase / Damjanovich, S., Kleppe, K.
Dátum:1967
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
analysis
Animal
Chromatography
enzymology
Glucosyltransferases
Molecular Weight
Muscles
Phosphorylase Kinase
Rabbits
Spectrophotometry
Sulfhydryl Compounds
Ultracentrifugation
Megjelenés:Biochemical and Biophysical Research Communications. - 26 : 1 (1967), p. 65-70. -
További szerzők:Kleppe, Kjell
Internet cím:elektronikus változat
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5.

001-es BibID:BIBFORM046274
Első szerző:Gáspár Rezső (biofizikus)
Cím:[Béta]-scorpion toxin 2 from Centruroides noxius blocks voltage-gated K+ channels in human lymphocytes / Gaspar R., Bene L., Damjanovich S., Munoz-Garay C., Calderon-Aranda E. S., Possani L. D.
Dátum:1995
ISSN:0006-291X
Megjegyzések:Using the patch-clamp technique, we determined that beta-scorpion toxin 2 from Centruroides noxius Hoffmann decreased whole-cell n-type K+ currents in human peripheral blood lymphocytes, with a half blocking concentration of approx. 5 microM. Toxin-2-accelerated inactivation, however, did not influence the kinetics of activation of the K+ conductance. The percentage increase in K+ channel inactivation rate and the degree of drug-induced block was independent of membrane potential. K+ channel block by Toxin 2 was instantaneous, not removable by washing with drug free extracellular solution. However, 10 mg/ml BSA in the bath lifted the toxin-induced block almost instantaneously and completely. Flow cytometric membrane potential measurements with the oxonol dye showed that Toxin 2 depolarizes human lymphocytes in concert with its K+ channel blocking effect.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:Biochemical And Biophysical Research Communications. - 213 : 2 (1995), p. 419-423. -
További szerzők:Bene László (1963-) (biofizikus) Damjanovich Sándor (1936-2017) (biofizikus) Munoz-Garay, Carlos Calderon-Aranda, Emma S. Possani, Lourival Domingos
Internet cím:Szerző által megadott URL
DOI
Intézményi repozitóriumban (DEA) tárolt változat
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6.

001-es BibID:BIBFORM046293
Első szerző:Gáspár Rezső (biofizikus)
Cím:Effect of acetylcholine on the electrophysiology and proliferative response of human lymphocytes / Gaspar R., Varga Z., Bene L., Marcheselli F., Pieri C., Damjanovich S.
Dátum:1996
ISSN:0006-291X
Megjegyzések:Using the patch-clamp technique, we determined that 1-15 mM extracellular acetylcholine reduced whole-cell n-type K+ currents in human peripheral blood lymphocytes and accelerated their inactivation. The percentage increase in K+ channel inactivation rate and the degree of drug induced block were independent of membrane potential. In flow cytometric membrane potential measurements with the oxonol dye similar doses of acetylcholine depolarized the lymphocyte population. Both acetylcholine induced K+ channel block and depolarization fully developed within 2 minutes. The depolarizing and K+ channel blocking effects of acetylcholine are in concert. [3H]thymidine incorporation experiments proved that the proliferative response of PHA stimulated peripheral blood lymphocytes was decreased by increasing concentrations of acetylcholine in the 1-50 mM range.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:Biochemical And Biophysical Research Communications. - 226 : 2 (1996), p. 303-308. -
További szerzők:Varga Zoltán (1969-) (biofizikus, szakfordító) Bene László (1963-) (biofizikus) Marcheselli, Fiorella Pieri, Carlo Damjanovich Sándor (1936-2017) (biofizikus)
Pályázati támogatás:T14655
OTKA
T13335
OTKA
6221
OTKA
Internet cím:Szerző által megadott URL
DOI
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7.

001-es BibID:BIBFORM046298
Első szerző:Goda Katalin (biofizikus)
Cím:Reversal of Multidrug Resistance by Valinomycin is Overcome by CCCP / Goda K., Krasznai Z., Gaspar R., Lankelma J., Westerhoff H. V., Damjanovich S., Szabo G.
Dátum:1996
ISSN:0006-291X
Megjegyzések:Reversal of P-glycoprotein-mediated multidrug resistance by valinomycin is overcome by the proton ionophore, CCCP. This effect, a complete suppression of the 5- to 10-fold valinomycin-induced reversal ("re-reversal"), exhibits a sharp extracellular potassium concentration ([K+(0)]) dependence. It is observed at [K+(0)] > 2-4 mM and not at [K+(0)] greater than or equal to 2 mM, in the case of the fluorescent substrates rhodamine 123 and daunorubicin. The fact that "re-reversal" is detected only for the combination of CCCP with valinomycin raises the possibility that a direct interaction between these ionophores may explain the phenomenon. We show spectroscopic evidence of such an interaction, with a [K+(0)]-dependence similar to that of the "re-reversal." These data suggest that the reversal of P-glycoprotein activity by valinomycin can be compromised by anionic compounds such as CCCP due to complex formation. More generally, molecular interactions involving P-glycoprotein substrates or reversing agents may significantly affect drug accumulation in multidrug resistant cells.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:Biochemical And Biophysical Research Communications. - 219 : 2 (1996), p. 306-310. -
További szerzők:Krasznai Zoltán (1950-) (biofizikus) Gáspár Rezső (1944-) (biofizikus) Lankelma, Jan Westerhoff, Hans V. Damjanovich Sándor (1936-2017) (biofizikus) Szabó Gábor (1953-) (biofizikus)
Pályázati támogatás:T14655
OTKA
17592
OTKA
Internet cím:Szerző által megadott URL
DOI
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8.

001-es BibID:BIBFORM006044
Első szerző:Matkó János (biológus)
Cím:Biphasic effect of extracellular ATP on the membrane potential of mouse thymocytes / Matko J., Nagy P., Panyi G., Vereb G. Jr., Bene L., Matyus L., Damjanovich S.
Dátum:1993
Megjegyzések:Extracellular ATP induced changes in the membrane potential of thymocytes from BALB/c mice were analyzed. At concentrations below 0.1 mM, ATP hyperpolarizes the cell membrane on the time scale of development of the Ca(2+)-signal. After a longer time hyperpolarization turns to depolarization. ATP concentrations higher than 0.5 mM caused rapid depolarization without previous hyperpolarization. Verapamil, quinine or the absence of extracellular Ca2+ blocked the hyperpolarization by ATP. In Na(+)-free medium the magnitude of depolarization decreased. Our data suggest a contribution of Ca(2+)-activated K+ channels to the hyperpolarizing effect of ATP at lower concentrations. The direction of membrane potential changes is determined presumably by a sensitive balance of ATP-receptor mediated Ca(2+)- and Na(+)-influx and the Ca(2+)-activated K(+)-channel activity.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Adenosine Triphosphate
Animal
Biophysics
Cell Membrane
cytology
drug effects
Hungary
Kinetics
Membrane Potentials
Mice
Mice,Inbred BALB C
pharmacology
physiology
Quinine
Support,Non-U.S.Gov't
Thymus Gland
Verapamil
Megjelenés:Biochemical and Biophysical Research Communications. - 191 : 2 (1993), p. 378-384. -
További szerzők:Nagy Péter (1971-) (biofizikus) Panyi György (1966-) (biofizikus) Vereb György (1965-) (biofizikus, orvos) Bene László (1963-) (biofizikus) Mátyus László (1956-) (biofizikus) Damjanovich Sándor (1936-2017) (biofizikus)
Internet cím:elektronikus változat
DOI
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9.

001-es BibID:BIBFORM006046
Első szerző:Mátyus László (biofizikus)
Cím:Voltage gating of Ca2(+)-activated potassium channels in human lymphocytes / Matyus, L., Pieri, C., Recchioni, R., Moroni, F., Bene, L., Tron, L., Damjanovich, S.
Dátum:1990
Megjegyzések:The effect of membrane potential on Ca2+ activated K+ channels was studied on human peripheral lymphocytes. Membrane potential was monitored using bisoxonol and flow cytometry. 1 mM Ca2+ in the presence of 2 microM ionomycin depolarized the control cell population, while 100 microM Ca2+ caused hyperpolarization. However 1 mM Ca2+ had a hyperpolarizing effect on previously partially depolarized cells. Potassium channel blockers did not influence the depolarization, while they inhibited the hyperpolarization. Based on the experimental evidence a voltage gating of Ca2+ activated K+ channels is suggested.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Biophysics
Calcium
drug effects
Flow Cytometry
Human
Hungary
In Vitro
Ion Channel Gating
Ionomycin
Leukocytes,Mononuclear
Lymphocytes
Membrane Potentials
pharmacology
physiology
Potassium
Potassium Channel Blockers
Potassium Channels
Support,Non-U.S.Gov't
Megjelenés:Biochemical and Biophysical Research Communications. - 171 : 1 (1990), p. 325-329. -
További szerzők:Pieri, Carlo Recchioni, Rina Moroni, Fausto Bene László (1963-) (biofizikus) Trón Lajos (1941-) (biofizikus) Damjanovich Sándor (1936-2017) (biofizikus)
Internet cím:elektronikus változat
DOI
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10.

001-es BibID:BIBFORM005090
Első szerző:Nagy Péter (biofizikus)
Cím:ICAM-1 inhibits the homocluster formation of MHC-I in colon carcinoma cells / Nagy, P., Vamosi, G., Damjanovich, S., Damjanovich, L.
Dátum:2006
ISSN:006-291X (Print)
Megjegyzések:ICAM-1 and MHC-I proteins play fundamental roles in antigen presentation, activation of T lymphocytes, and immune responses against tumor cells. Both of them participate in the formation of lipid raft-associated membrane protein clusters. We found significant colocalization between ICAM-1 and MHC-I at the level of large-scale associations. We combined RNA interference and fluorescence resonance energy transfer studies to show that ICAM-1 promotes the partial disassembly of MHC-I homoclusters on LS-174T colon carcinoma cells. Interferon-gamma (IFN-gamma) treatment induced an increase in the expression of MHC-I and ICAM-1 resulting in decreased MHC-I homoassociation. Small interfering RNAs directed against ICAM-1 restored the homoassociation of MHC-I without influencing the expression level of MHC-I by eliminating ICAM-1 molecules interspersed in MHC-I clusters. We conclude that the composition of membrane protein clusters is dynamically altered in response to both physiological and experimentally elicited changes in antigen expression levels.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Antigen Presentation
Antigens
Biophysics
Carcinoma
Cell Line, Tumor
Cells
Colonic Neoplasms
Energy Transfer
Fluorescence
Fluorescence Resonance Energy Transfer
genetics
Histocompatibility Antigens
Histocompatibility Antigens Class I
Humans
Hungary
Intercellular Adhesion Molecule-1
Lymphocytes
metabolism
Protein Binding
Proteins
Research
RNA,Small Interfering
Support
T-Lymphocytes
Megjelenés:Biochemical and Biophysical Research Communications. - 347 : 3 (2006), p. 758-763. -
További szerzők:Vámosi György (1967-) (biofizikus) Damjanovich Sándor (1936-2017) (biofizikus) Damjanovich László (1960-) (általános sebész)
Internet cím:DOI
elektronikus változat
Borító:

11.

001-es BibID:BIBFORM046300
Első szerző:Panyi György (biofizikus)
Cím:Immunosuppressors inhibit voltage-gated potassium channels in human peripheral blood lymphocytes / Panyi G., Gaspar R., Krasznai Z., ter Horst J. J., Ameloot M., Aszalos A., Steels P., Damjanovich S.
Dátum:1996
ISSN:0006-291X
Megjegyzések:The effects of immunosuppressive agents on the potassium current of human peripheral blood lymphocytes have been studied using the whole-cell patch-clamp technique. Cyclosporin A (10 micrograms/ml), rapamycin (10 micrograms/ml) and FK-506 (2.5 micrograms/ml) reduced the peak K+ current by approximately 40, 30 and 40% of the control, respectively, without any change in the reversal potential of the current. The current inhibition was similar at all membrane potentials studied and was accompanied with an increase in the rate of K+ current inactivation. Membrane potential measurements in current-clamp showed a marked depolarization of the membrane (>10 mV) upon the addition of either immunosuppressor to the cells. Our findings revealed that the voltage-dependent potassium current in human peripheral blood lymphocytes is inhibited by Cyclosporin A and other immunosuppressors, resulting in a depolarized membrane potential.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:Biochemical and Biophysical Research Communications. - 221 : 2 (1996), p. 254-258. -
További szerzők:Gáspár Rezső (1944-) (biofizikus) Krasznai Zoltán (1950-) (biofizikus) ter Horst, Jan J. Ameloot, Marcel Aszalos Adorján Steels, Paul Damjanovich Sándor (1936-2017) (biofizikus)
Pályázati támogatás:1459
OTKA
1492
OTKA
6221
OTKA
E12533
OTKA
T14655
OTKA
F13335
OTKA
Internet cím:Intézményi repozitóriumban (DEA) tárolt változat
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12.

001-es BibID:BIBFORM046287
Első szerző:Panyi György (biofizikus)
Cím:Peripheral blood lymphocytes display reduced K+ channel activity in aged humans / Panyi G., Berecki G., Gaspar R., Seres I., Fulop T., Damjanovich S.
Dátum:1994
ISSN:0006-291X
Megjegyzések:Steady-state parameters of whole-cell K+ current have been determined in human peripheral blood lymphocytes of young (20-50 y.) and elderly (> 90 y.) volunteers by patch-clamp. The magnitude and voltage dependence of the K+ conductance were similar in both lymphocyte populations. The midpoint of steady-state inactivation was -53.3 +/- 2.3 mV for lymphocyte population of young individuals and -65.0 +/- 3.0 mV for that of elderly, showing a significant shift to hyperpolarized potentials. The peak of the steady-state open probability of the K+ channels was decreased and shifted to depolarized potentials by approx. 12.5 mV for lymphocytes of elderly donors. It is suggested that the observed differences in the K+ current parameters may be at least partly responsible for the impaired responsiveness of elderly lymphocytes to proliferative stimuli.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:Biochemical and Biophysical Research Communications. - 199 : 2 (1994), p. 519-524. -
További szerzők:Berecki Géza Gáspár Rezső (1944-) (biofizikus) Seres Ildikó (1954-) (biokémikus) Fülöp Tamás (1928-) (népegészségügyi szakember, egészségfejlesztő) Damjanovich Sándor (1936-2017) (biofizikus)
Internet cím:Intézményi repozitóriumban (DEA) tárolt változat
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