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001-es BibID:BIBFORM006039
035-os BibID:PMID:2317496
Első szerző:Lakos Zsuzsa (biofizikus)
Cím:The effect of transmembrane potential on the dynamic behavior of cell membranes / Z. Lakos, B. Somogyi, M. Balazs, J. Matko, S. Damjanovich
Dátum:1990
ISSN:0005-2736
Megjegyzések:The relationship between transmembrane potential and lipid dynamics in the cytoplasmic membrane of mouse thymus cells has been investigated. Changes of transmembrane potential was followed by measuring the fluorescence emission of the anionic dye, bis-(1,3-dibutylbarbiturate)trimethine oxonol (diBa-C4-(3)). Assessment of lipid fluidity was carried out applying three fluorescent lipid probes, 1-[4-(trimethylammonium)phenyl]-6-phenyl-1,3,5-hexatriene (TMA-DPH), 12-(9-anthroyloxy)stearic acid (12-AS) and 1,6-diphenyl-1,3,5-hexatriene (DPH) used to monitor different structural regions of the bilayer. The fluorescence anisotropy of these probes was measured as a function of temperature at two values of transmembrane potential. In the case of DPH it proved to depend on the membrane potential in the higher temperature range (above 28 degrees C), while no such a dependence could be observed for DPH below this temperature range and for TMA-DPH and 12-AS in between 20 and 37 degrees C. These data suggest that changes in transmembrane potential are accompanied with some local alteration in membrane lipid dynamics and/or structure
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Cell membrane potential
Membrane fluidity
Lipid packing
Fluorescence
Fluorescence anisotropy
Megjelenés:Biochimica et Biophysica Acta (BBA). Biomembranes. - 1023 : 1 (1990), p. 41-46. -
További szerzők:Somogyi Béla (1945-2006) (biofizikus) Balázs Margit (1952-) (sejtbiológus, molekuláris genetikus) Matkó János (1952-) (biológus) Damjanovich Sándor (1936-2017) (biofizikus)
Internet cím:Intézményi repozitóriumban (DEA) tárolt változat
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001-es BibID:BIBFORM043988
Első szerző:Szabó Gábor (biofizikus)
Cím:Light-induced permeabilization and merocyanine 540 staining of mouse spleen cells / Szabo Gábor, Rédai Imre, Bacso Zsolt, Hevessy Jozsef, Damjanovich Sándor
Dátum:1989
ISSN:0005-2736
Megjegyzések:Merocyanine 540 (M540) is a potential-sensitive, hydrophobic dye that preferentially incorporates into the 'fluid' domains of cellular membranes, distinguishing between hemopoietic cells according to their differentiation state. A bright staining with M540 is usually achieved by UV illumination of the cells during staining. We show by flow cytometric analysis that: (1) staining is greatly enhanced by UV illumination of mouse spleen cells before addition of the dye; (2) UV treatment causes an increased permeability toward propidium iodide and intracellular fluorescein as well; (3) the increment in M540 fluorescence precedes permeabilization to propidium iodide, while the latter precedes leakage of fluorescein. We also describe an overshoot and accelerated recovery of M540 fluorescence after photobleaching by a 514 nm laser beam. It is suggested that penetration of M540 to the more fluid inner membrane structures explains the fluorescence increment in both experiments.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:Biochimica et Biophysica Acta (BBA). Biomembranes. - 979 : 3 (1989), p. 365-370. -
További szerzők:Rédai Imre (1961-) (sebész, aneszteziológus) Bacsó Zsolt (1963-) (biofizikus) Hevessy József Damjanovich Sándor (1936-2017) (biofizikus)
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DOI
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