Találati lista | Tudóstér

001-es BibID:	BIBFORM004858
Első szerző:	Bene László (biofizikus)
Cím:	Detection of receptor trimers on the cell surface by flow cytometric fluorescence energy homotransfer measurements / László Bene, János Szöllősi, Gergely Szentesi, László Damjanovich, Rezső Gaspar, Thomas A. Waldmann, Sándor Damjanovich
Dátum:	2005
ISSN:	0006-3002
Megjegyzések:	Fluorescence energy homotransfer offers a powerful tool for the investigation of the state of oligomerization of cell surface receptors on a cell-by-cell basis by measuring the polarized components of fluorescence intensity of cells labeled with fluorescently stained antibodies. Here we describe homotransfer-based methods for the flow cytometric detection and analysis of hetero- and homo-associations of cell surface receptors. Homotransfer efficiencies for two- and three-body energy transfer interactions are defined and their frequency distribution curves are computed from the fluorescence anisotropy distributions of multiple-labeled cells. The fractions of receptors involved in homo-clustering is calculated based on the dependence of the fluorescence anisotropy on the surface concentration of the fluorescently stained antibodies. A homotransfer analysis of the homo- and hetero-clustering of the MHCI and MHCII glycoproteins, the cytokine receptor IL-2Ralpha, transferrin receptor and the receptor-type tyrosine phosphatase CD45 on JY B and Kit-225-K6 T cells is presented. We investigated how various factors such as the type of dye, rotational mobility of the dye and dye-targeting antibody, as well as the wavelength of the exciting light affect the homotransfer. We show that the homotransfer technique combined with the high statistical resolution of flow cytometry is an effective tool for detecting different oligomeric states of receptors by using fluorophores having restricted rotational mobility on the time scale of fluorescence
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban Fluorescence anisotropy Receptor clustering MHCI and MHCII glycoprotein IL-2Rα Transferrin receptor CD45
Megjelenés:	Biochimica et Biophysica Acta (BBA). Molecular Cell Research. - 1744 : 2 (2005), p. 176-198. -
További szerzők:	Szöllősi János (1953-) (biofizikus) Szentesi Gergely (1976-) (kémia-fizika tanár) Damjanovich László (1960-) (általános sebész) Gáspár Rezső (1944-) (biofizikus) Waldmann, Thomas A. Damjanovich Sándor (1936-2017) (biofizikus)
Internet cím:	Intézményi repozitóriumban (DEA) tárolt változat DOI
Borító:
Saját polcon:

001-es BibID:	BIBFORM006034
Első szerző:	Gáspár Rezső (biofizikus)
Cím:	Bretylium-induced voltage-gated sodium current in human lymphocytes / Rezső Gáspár, Zoltán Krasznai, Teréz Márián, Lajos Trón, Rina Recchioni, Marco Falasca, Fausto Moroni, Carlo Pieri, Sándor Damjanovich
Dátum:	1992
ISSN:	0167-4889
Megjegyzések:	Using the whole-cell variation of the patch-clamp technique it has been determined that 0.25-3 mM bretylium tosylate (BT) exerts a repolarizing effect on partially depolarized human lymphocytes. The repolarizing effect was ouabain (40 microM)-sensitive, and was inhibited by the removal of external Na+ or by the Na(+)-channel-blocker amiloride (10-44 microM), but K(+)-channel-blockers 4-aminopyridine (0.1-5 mM) and quinine (100 microM) had no effect. The drug induced a sodium dependent, amiloride-sensitive transient inward current reaching its maximum value approx. 20-30 s after the administration of BT and lasting for 6-10 min. This current was activated by depolarization within 25 ms at around -42 mV, its inactivation took about 2 s and its reversal potential was +24 +/- 5 mV. An increase in the intracellular sodium concentration (1.8-3.2 mM) has been observed upon the addition of BT by monitoring the SBFI fluorescence of the dye-loaded cells. It has been shown that whole-cell K+ currents are significantly decreased by BT. The existence of voltage and ligand (BT)-gated sodium channels has been postulated in human lymphocytes. These channels are thought to participate in the initiation of membrane repolarization in human lymphocytes, and thereby influence mitogenic or antigen-induced cell-activation processes.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban Bretylium Ion channel Patch-clamp Human lymphocyte
Megjelenés:	Biochimica et Biophysica Acta (BBA). Molecular Cell Research. - 1137 : 2 (1992), p. 143-147. -
További szerzők:	Krasznai Zoltán (1950-) (biofizikus) Márián Teréz (1950-) (radiobiológus) Trón Lajos (1941-) (biofizikus) Recchioni, Rina Falasca, Marco Moroni, Fausto Pieri, Carlo Damjanovich Sándor (1936-2017) (biofizikus)
Internet cím:	Intézményi repozitóriumban (DEA) tárolt változat DOI Szerző által megadott URL
Borító:
Saját polcon:

001-es BibID:	BIBFORM005985
035-os BibID:	PM:3011116
Első szerző:	Mátyus László (biofizikus)
Cím:	Cyclosporin A depolarizes cytoplasmic membrane potential and interacts with Ca2+ ionophores / László Mátyus, Margit Balázs, Adorján Aszalós, Sally Mulhern, Sándor Damjanovich
Dátum:	1986
ISSN:	0167-4889
Megjegyzések:	Cytoplasmic membrane potential of mouse lymphocytes was determined with flow cytometry and fluorescence spectroscopy using 3,3'-dihexylcarbocyanine iodide (DiOC6(3)). The amount of this lipophilic cation incorporated into the cytoplasmic membrane is dependent upon the transmembrane potential, so the dye is suitable for continuous monitoring of this parameter, under controlled conditions. Membrane potential of the cells was decreased in the presence of cyclosporin A and cyclosporin G in a dose-dependent manner. However, the depolarization caused by Ca2+ ionophores, ionomycin and A23187, was reduced in the presence of cyclosporin A. Electron spin resonance spectroscopy with 5-doxylstearic acid as a probe indicated that cyclosporin A decreased the apparent motional freedom of membrane lipids. These data suggest incorporation of cyclosporin A into the cytoplasmic membrane, causing changes in ion fluxes. The membrane potential change induced by cyclosporin A may have selective biological consequences in certain subpopulations of lymphocytes.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban Animal Calcimycin Carbocyanines Cell Membrane Cyclosporins Dose-Response Relationship,Drug drug effects Drug Interactions Electron Spin Resonance Spectroscopy Ethers Flow Cytometry Fluorescence Human Insulin Ionomycin Ionophores Lymphocyte Transformation Lymphocytes Membrane Lipids Membrane Potentials metabolism Mice Mice,Inbred Strains pharmacology Quinolines ultrastructure
Megjelenés:	Biochimica et Biophysica Acta (BBA). Molecular Cell Research. - 886 : 3 (1986), p. 353-360. -
További szerzők:	Balázs Margit (1952-) (sejtbiológus, molekuláris genetikus) Aszalos Adorján Mulhern, Sally Damjanovich Sándor (1936-2017) (biofizikus)
Internet cím:	Intézményi repozitóriumban (DEA) tárolt változa DOI Szerző által megadott URL
Borító:
Saját polcon: