CCL

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1.

001-es BibID:BIBFORM004939
Első szerző:Bene László (biofizikus)
Cím:Major histocompatibility complex class I protein conformation altered by transmembrane potential changes / Bene, L., Szollosi, J., Balazs, M., Matyus, L., Gaspar, R., Ameloot, M., Dale, R. E., Damjanovich, S.
Dátum:1997
ISSN:0196-4763
Megjegyzések:The nature of charge distributions in membrane-bound macromolecular structures renders them susceptible to interaction with transmembrane potential fields. As a result, conformational changes in such species may be expected to occur when this potential is altered. We have detected reversible conformational change in the major histocompatibility complex (MHC) class I antigen in the plasma membrane of human JY cells, as monitored by flow-cytometric resonance energy-transfer, upon reduction of the transmembrane potential (depolarization). This change increased the intramolecular energy-transfer efficiency between fluorescent donor- and acceptor-labeled monoclonal antibodies directed, respectively, to epitopes on the light (beta 2-microglobulin) and the heavy chains of the MHC class I antigen. Repolarization of the depolarized samples restored the energy-transfer efficiency to the original values measured before depolarization. Depolarization caused similar relative changes in fluorescence resonance energy-transfer efficiency when Fab fragments were used for labeling MHC class I complex, suggesting that the observed phenomenon is not restricted to whole monoclonal antibodies.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Antibodies, Monoclonal
Antigen Presentation
B-Lymphocytes
beta 2-Microglobulin
Cell Membrane
chemistry
cytology
Dyes
Energy Transfer
Enzyme Activation
Flow Cytometry
Fluorescein-5-isothiocyanate
Fluorescence
Fluorescent Dyes
Histocompatibility Antigens Class I
Human
Hungary
immunology
Light
Major Histocompatibility Complex
Membrane Potentials
metabolism
methods
Na(+)-K(+)-Exchanging ATPase
Patch-Clamp Techniques
physiology
Protein Conformation
Rhodamines
Surface Properties
Megjelenés:Cytometry. - 27 : 4 (1997), p. 353-357. -
További szerzők:Szöllősi János (1953-) (biofizikus) Balázs Margit (1952-) (sejtbiológus, molekuláris genetikus) Mátyus László (1956-) (biofizikus) Gáspár Rezső (1944-) (biofizikus) Ameloot, Marcel Dale, Robert E. Damjanovich Sándor (1936-2017) (biofizikus)
Internet cím:elektronikus változat
Borító:

2.

001-es BibID:BIBFORM004657
Első szerző:Bene László (biofizikus)
Cím:Detection of receptor clustering by flow cytometric fluorescence anisotropy measurements / Bene, L., Fulwyler, M. J., Damjanovich, S.
Dátum:2000
Megjegyzések:Perrin equation suggests an alternative way for the accurate energy transfer determination on a cell-by-cell basis by measuring polarized donor intensities in a conventional flow cytometer. METHODS: The relationship between energy transfer and fluorescence anisotropy of the donor was investigated by flow cytometric generation of Perrin-lifetime plots of fluorescent antibody-labeled MHC class I and class II molecules on the surface of living cells. The energy transfer reduced the fluorescence lifetime of the donor. RESULTS: Perrin plots have proven to be sensitive to the segmental mobility of the labeling dye and that of antibodies of different isotypes, and homo-transfer due to the multiple labeling of antibodies. A method demonstrating the feasibility of energy transfer determination by measuring anisotropy enhancement of the donor is presented. Flow cytometric histograms of the donor anisotropy and of the deduced energy transfer efficiency are shown, indicating clustering of MHC class I and class II molecules on the surface of human T lymphoblasts. In the Appendix, a method for the simultaneous determination of both energy transfer efficiency and donor fluorescence anisotropy, without need for G-factor measurement, is also presented. CONCLUSIONS: We demonstrate that energy transfer efficiency, i.e., proximity, between suitably selected donor and acceptor, and the rotational relaxation of the donor, i.e., donor mobility, can be simultaneously measured in a flow cytometer
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Adult
analysis
Antibodies
beta 2-Microglobulin
Cells
diagnostic use
Dyes
Energy Transfer
Flow Cytometry
Fluorescence
Fluorescence Polarization
Fluorescent Dyes
Histocompatibility Antigens Class I
Histocompatibility Antigens Class II
Human
Hungary
Immunoglobulin G
immunology
methods
Receptors,Cell Surface
Support,Non-U.S.Gov't
T-Lymphocytes
Megjelenés:Cytometry. - 40 : 4 (2000), p. 292-306. -
További szerzők:Fulwyler, Mack J. Damjanovich Sándor (1936-2017) (biofizikus)
Internet cím:elektronikus változat
DOI
Borító:

3.

001-es BibID:BIBFORM004628
035-os BibID:(scopus)0032190410 (wos)000076205700017
Első szerző:Damjanovich Sándor (biofizikus)
Cím:Supramolecular receptor structures in the plasma membrane of lymphocytes revealed by flow cytometric energy transfer, scanning force- and transmission electron-microscopic analyses / Damjanovich, S., Matko, J., Matyus, L., Szabo, G. jr., Szollosi, J., Pieri, J. C., Farkas, T., Gaspar, R.
Dátum:1998
ISSN:196-4763
Megjegyzések:Receptors in the plasma membrane of blood cells in general and in that of lymphocytes in particular are supposed to move around in a random walk fashion relatively freely driven by thermal diffusion, as described by the Singer-Nicolson fluid mosaic membrane model. In this article we summarized data and techniques that indicated nonrandom codistribution patterns of receptor superstructures under conditions, where the generation of such molecular colocalizations by the methods themselves were excluded. Application of fluorescence energy transfer in a flow cytometer helped to analyze such codistribution patterns in cell populations. After normalizing energy transfer values for possible differences between labeling ratios of the targeting monoclonal antibodies and using the mean values of energy transfer distribution curves, two-dimensional receptor maps were generated from data obtained in a pair-wise fashion between receptors. Major histocompatibility complex (MHC) class I and II, intercellular adhesion molecule-1 (ICAM-1), TcR-CD3-CD4, tetraspan molecules (CD81, CD82, CD53), and the subunits of the multisubunit IL-2 receptor displayed nonrandom codistribution patterns sometimes with, but very frequently without induction by their ligand. Immunogold-bead "sandwich" labeling analyzed by atomic force microscopy has shown that such receptor "islands" existed also in "receptor-island-groups". This indicated the existence of nonrandom receptor distribution of MHC class I and II molecules also at an elevated hierarchical level. An analysis is given herein concerning a standardized approach. The apparent incompatibility of these supramolecular patterns with the Singer-Nicolson type "free-protein and lipid-mobility paradigm" was resolved by recommending an additional emphasis on the mosaicism of the membrane besides receptor mobility.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
analysis
Antibodies
Antigens
Antigen
blood
Monoclonal
chemistry
Diffusion
Dyes
Energy Transfer
Flow Cytometry
Fluorescence
Fluorescent Dyes
HLA Antigens
Human
Hungary
Immunoglobulins
Fab
Immunohistochemistry
immunology
Intercellular Adhesion Molecule-1
Interleukin-2
Lymphocytes
Macromolecular Systems
Major Histocompatibility Complex
Membrane Fluidity
methods
Microscopy
Atomic Force
Microspheres
Modelsl
Motion
Receptor-CD3 Complex
T-Cell
Receptors
Cell Surface
Support
Non-U.S.Gov't
Tumor Cells
Cultured
Megjelenés:Cytometry. - 33 : 2 (1998), p. 225-233. -
További szerzők:Matkó János (1952-) (biológus) Mátyus László (1956-) (biofizikus) Szabó Gábor (1953-) (biofizikus) Szöllősi János (1953-) (biofizikus) Pieri, J. C. Farkas Tamás (1971-) (biológus) Gáspár Rezső (1944-) (biofizikus)
Internet cím:DOI
elektronikus változat
Borító:

4.

001-es BibID:BIBFORM042481
035-os BibID:(scopus)40049093067 (wos)000253576200006
Első szerző:Fazekas Zsolt (biofizikus)
Cím:Two-sided fluorescence resonance energy transfer for assessing molecular interactions of up to three distinct species in confocal microscopy / Zsolt Fazekas, Miklós Petrás, Ákos Fábián, Zsuzsanna Pályi-Krekk, Péter Nagy, Sándor Damjanovich, György Vereb, János Szöllősi
Dátum:2008
ISSN:1552-4922 1552-4930
Megjegyzések:The role of the expression patterns of proteins involved in oncogenesis can be understood after characterizing their multimolecular interactions. Conventional FRET methods permit the analysis of interaction between two molecular species at the most, which necessitates the introduction of new approaches for studying multicomponent signaling complexes. Flow cytometric as well as microscopic donor (dbFRET) and acceptor (abFRET) photobleaching FRET measurements were performed to determine the association states of ErbB2, b1-integrin, and CD44 receptors. Based on consecutively applied abFRET and dbFRET methods (two-sided FRET), the relationship of b1-integrin?ErbB2 heteroassociation to ErbB2 homoassociation and of b1-integrin?ErbB2 heteroassociation to ErbB2?CD44 heteroassociation was studied by correlating pixel-by-pixel FRET values of the corresponding abFRET and dbFRET images in contour plots. Anticorrelation was observed between b1-integrin?ErbB2 heteroassociation and ErbB2 homoassociation on trastuzumab sensitive N87 and SK-BR-3 cells, while modest positive correlation was found between b1-integrin?ErbB2 and ErbB2?CD44 heteroassociationon trastuzumab resistant MKN-7 cells. The FRET efficiency values of b1-integrin?ErbB2 heteroassociation were markedly higher at the focal adhesion regions on attachedcells than those measured by flow cytometry on detached cells. In conclusion, we implemented an experimental set-up termed two-sided FRET for correlating two pairwiseinteractions of three arbitrarily chosen molecular species. On the basis of our results, we assume that the homoassociation state of ErbB2 is dynamically modulated by its interaction with b1-integrins.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
beta1-integrin
abFRET
analysis
Biophysics
Carcinoma
CD44
Cell Adhesion
Cell Adhesion Molecules
Cell Line
Cells
Cholera Toxin
Confocal
Confocal microscopy
cytology
dbFRET
Energy Transfer
ErbB2
Flow Cytometry
Fluorescein
Fluorescence
fluorescence microscopy
Fluorescence Resonance Energy Transfer
FRET
Hiv-1
Human
Hungary
Image Cytometry
Image Processing
Kinetics
Laser scanning confocal microscopy
Luminescence
methods
Microscopy
Photobleaching
Protein-protein interactions
Proteins
Receptor tyrosine kinase
Research
Signal Transduction
Software
therapy
Trastuzumab resistance
tsFRET
Tyrosine
egyetemen (Magyarországon) készült közlemény
Megjelenés:Cytometry Part A. - 73 : 3 (2008), p. 209-219. -
További szerzők:Petrás Miklós (1977-) (orvos) Fábián Ákos István (1982-) (aneszteziológus) Pályiné Krekk Zsuzsanna (1974-) (molekuláris biológus) Nagy Péter (1971-) (biofizikus) Damjanovich Sándor (1936-2017) (biofizikus) Vereb György (1965-) (biofizikus, orvos) Szöllősi János (1953-) (biofizikus)
Internet cím:Szerző által megadott URL
DOI
Intézményi repozitóriumban (DEA) tárolt változat
Borító:

5.

001-es BibID:BIBFORM004878
035-os BibID:(scopus)26444586247 (wos)000232299300007
Első szerző:Nagy Péter (biofizikus)
Cím:Novel calibration method for flow cytometric fluorescence resonance energy transfer measurements between visible fluorescent proteins / Nagy, P., Bene, L., Hyun, W. C., Vereb, G., Braun, M., Antz, C., Paysan, J., Damjanovich, S., Park, J. W., Szollosi, J.
Dátum:2005
ISSN:1552-4922
Megjegyzések:The combination of fluorescence resonance energy transfer (FRET) and flow cytometry offers a statistically firm approach to study protein associations. Fusing green fluorescent protein (GFP) to a studied protein usually does not disturb the normal function of a protein, but quantitation of FRET efficiency calculated between GFP derivatives poses a problem in flow cytometry. METHODS: We generated chimeras in which cyan fluorescent protein (CFP) was separated by amino acid linkers of different sizes from yellow fluorescent protein (YFP) and used them to calibrate the cell-by-cell flow cytometric FRET measurements carried out on two different dual-laser flow cytometers. Then, CFP-Kip1 was coexpressed in yeast cells with YFP and cyclin-dependent kinase-2 (Cdk2) and served as a positive control for FRET measurements, and CFP-Kip1 coexpressed with a random peptide fused to YFP was the negative control. RESULTS: We measured donor, direct, and sensitized acceptor fluorescence intensities and developed a novel way to calculate a factor (alpha) that characterized the fluorescence intensity of acceptor molecules relative to the same number of excited donor molecules, which is essential for quantifying FRET efficiency. This was achieved by calculating FRET efficiency in two different ways and minimizing the squared difference between the two results by changing alpha. Our method reliably detected the association of Cdk2 with its inhibitor, Kip1, whereas the nonspecific FRET efficiency between Cdk2 and a random peptide was negligible. We identified and sorted subpopulations of yeast cells showing interaction between the studied proteins. CONCLUSIONS: We have described a straightforward novel calibration method to accurately quantitate FRET efficiency between GFP derivatives in flow cytometry
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
analysis
Biophysics
Calibration
Cells
chemistry
Cyclin-Dependent Kinase 2
Cyclin-Dependent Kinase Inhibitor p27
Energy Transfer
Flow Cytometry
Fluorescence
Fluorescence Resonance Energy Transfer
Hungary
metabolism
methods
Protein Binding
Proteins
Research
Saccharomyces cerevisiae
Saccharomyces cerevisiae Proteins
Support
Megjelenés:Cytometry. Part A. - 67 : 2 (2005), p. 86-96. -
További szerzők:Bene László (1963-) (biofizikus) Hyun, William C. Vereb György (1965-) (biofizikus, orvos) Braun, Manuel Antz, Christof Paysan, Jacques Damjanovich Sándor (1936-2017) (biofizikus) Park, John W. Szöllősi János (1953-) (biofizikus)
Internet cím:elektronikus változat
DOI
Borító:

6.

001-es BibID:BIBFORM004634
035-os BibID:(scopus)0032101894 (wos)000073937700007
Első szerző:Nagy Péter (biofizikus)
Cím:EGF-induced redistribution of erbB2 on breast tumor cells : flow and image cytometric energy transfer measurements / Nagy, P., Bene, L., Balazs, M., Hyun, W. C., Lockett, S. J., Chiang, N. Y., Waldman, F., Feuerstein, B. G., Damjanovich, S., Szollosi, J.
Dátum:1998
Megjegyzések:erbB2, a member of the epidermal growth factor (EGF) receptor-type tyrosine kinase receptor family, is overexpressed in breast carcinomas with poor prognosis. We examined the cell surface association of this receptor with itself and with other cell surface proteins by the Forster-type fluorescence resonance energy transfer using whole antibodies and Fab fragments. We found that erbB2 molecules homoassociate in unstimulated SK-BR-3, BT474 and BT474-M (a metastatic version of the parent BT474 line) breast tumor cells, and that the interaction was enhanced by EGF treatment in suspensions of SK-BR-3 and BT474-M cells. BT474 cells (with low EGF receptor expression) and attached SK-BR-3 cells do not respond to EGF. Image microscopic energy transfer measurements found considerable pixel-by-pixel heterogeneity in the homoassociation state of erbB2. In accordance with the EGF-induced redistribution of erbB2, EGF receptor was found to be in close proximity to erbB2 in FRET measurements. By labeling different epitopes on erbB2 and the lipid bilayer, we were able to prepare an epitope map of erbB2 molecule. Our data suggest the existence of dynamic cell surface patterns of erbB2 and point to functions fulfilled by these molecular complexes.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
analysis
biosynthesis
Breast Neoplasms
Carcinoma
Cell Membrane
chemistry
drug effects
Energy Transfer
Epidermal Growth Factor
Female
Flow Cytometry
Fluorescence
Human
Hungary
metabolism
methods
Models
Molecular
pharmacology
Receptor
erbB-2
Receptors
Transferrin
Support
Non-U.S.Gov't
Tumor Cells,Cultured
Squamous Cell
ultrastructure
Megjelenés:Cytometry. - 32 : 2 (1998), p. 120-131. -
További szerzők:Bene László (1963-) (biofizikus) Balázs Margit (1952-) (sejtbiológus, molekuláris genetikus) Hyun, William C. Lockett, Steven J. Chiang, Nancy Y. Waldman, Frederick Feuerstein, Burt G. Damjanovich Sándor (1936-2017) (biofizikus) Szöllősi János (1953-) (biofizikus)
Internet cím:elektronikus változat
DOI
Borító:

7.

001-es BibID:BIBFORM006003
Első szerző:Szabó Gábor (biofizikus)
Cím:Flow cytometric analysis of the uptake of Hoechst 33342 dye by human lymphocytes / Gábor Szabó, Attila Kiss, Sándor Damjanovich
Dátum:1981
Megjegyzések:Human peripheral blood lymphocytes have been shown to resist staining with the DNA binding fluorochrome Hoechst 33342 by the cellular membrane. The rate of uptake of the dye is strongly temperature-dependent with minimal uptake rate below 16 degrees C. The activation energy of dye transport was found to be 135 kJ/mol above 20 degrees C and about 20 kJ/mol below 16 degrees C. Metabolic inhibitors accelerated, instead of inhibiting, the transport of the dye. Dead cells have been shown to stain promptly in contrast with the gradually staining viable cells. The uptake process in the vital staining conditions is suggested to involve a carrier mediated mechanism. Application of Hoechst 33342 as a fluorescent indicator of viability is proposed.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
analysis
Azides
Benzimidazoles
Biological Transport
blood
Cell Survival
Dna
Dyes
Flow Cytometry
Fluorescent Dyes
Human
Kinetics
Lymphocytes
metabolism
pharmacology
Sodium
Sodium Azide
Staining and Labeling
Support,Non-U.S.Gov't
Temperature
Megjelenés:Cytometry. - 2 : 1 (1981), p. 20-23. -
További szerzők:Kiss Attila (1942-) (belgyógyász, haematológus) Damjanovich Sándor (1936-2017) (biofizikus)
Internet cím:elektronikus változat
DOI
Borító:

8.

001-es BibID:BIBFORM006000
035-os BibID:(scopus)0024307312 (wos)A1989CA57200018
Első szerző:Szabó Gábor (biofizikus)
Cím:Fluorescent staphylococci as microbeads / G. Szabó, S. Damjanovich
Dátum:1989
Megjegyzések:Fixed bacteria of the protein A-rich Cowan I Staphylococcus strain were labelled with fluorescein isothiocyanate and used as the second-step reagent in an indirect immunofluorescent assay of specific cell-surface antigen expression. The results are documented with fluorescence microscopy and flow cytometry.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Animal
diagnostic use
Flow Cytometry
Fluorescence
Fluorescent Antibody Technique
Hungary
methods
Microscopy
Microscopy,Fluorescence
Microspheres
Staphylococcal Protein A
Staphylococcus
Megjelenés:Cytometry. - 10 : 6 (1989), p. 801-802. -
További szerzők:Damjanovich Sándor (1936-2017) (biofizikus)
Internet cím:elektronikus változat
DOI
Borító:

9.

001-es BibID:BIBFORM004886
Első szerző:Szentesi Gergely (kémia-fizika tanár)
Cím:Computer program for analyzing donor photobleaching FRET image series / Szentesi, G., Vereb, G., Horvath, G., Bodnar, A., Fabian, A., Matko, J., Gaspar, R., Damjanovich, S., Matyus, L., Jenei, A.
Dátum:2005
ISSN:1552-4922
Megjegyzések:The photobleaching fluorescence resonance energy transfer (pbFRET) technique is a spectroscopic method to measure proximity relations between fluorescently labeled macromolecules using digital imaging microscopy. To calculate the energy transfer values one has to determine the bleaching time constants in pixel-by-pixel fashion from the image series recorded on the donor-only and donor and acceptor double-labeled samples. Because of the large number of pixels and the time-consuming calculations, this procedure should be assisted by powerful image data processing software. There is no commercially available software that is able to fulfill these requirements. METHODS: New evaluation software was developed to analyze pbFRET data for Windows platform in National Instrument LabVIEW 6.1. This development environment contains a mathematical virtual instrument package, in which the Levenberg-Marquardt routine is also included. As a reference experiment, FRET efficiency between the two chains (beta2-microglobulin and heavy chain) of major histocompatibility complex (MHC) class I glycoproteins and FRET between MHC I and MHC II molecules were determined in the plasma membrane of JY, human B lymphoma cells. RESULTS: The bleaching time constants calculated on pixel-by-pixel basis can be displayed as a color-coded map or as a histogram from raw image format. CONCLUSION: In this report we introduce a new version of pbFRET analysis and data processing software that is able to generate a full analysis pattern of donor photobleaching image series under various conditions.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Algorithms
analysis
beta 2-Microglobulin
Biophysics
Cell Line,Tumor
Cells
Energy Transfer
Fluorescence
Fluorescence Resonance Energy Transfer
Glycoproteins
Histocompatibility Antigens
Human
Humans
Hungary
Lymphoma
Major Histocompatibility Complex
metabolism
methods
Microscopy
Photobleaching
Research
Software
Support
egyetemen (Magyarországon) készült közlemény
Megjelenés:Cytometry. Part A. - 67 : 2 (2005), p. 119-128. -
További szerzők:Vereb György (1965-) (biofizikus, orvos) Horváth Gábor (1974-) (biofizikus) Dóczy-Bodnár Andrea (1970-) (biofizikus) Fábián Ákos István (1982-) (aneszteziológus) Matkó János (1952-) (biológus) Gáspár Rezső (1944-) (biofizikus) Damjanovich Sándor (1936-2017) (biofizikus) Mátyus László (1956-) (biofizikus) Jenei Attila (1966-) (biofizikus)
Internet cím:elektronikus változat
DOI
Borító:

10.

001-es BibID:BIBFORM006016
Első szerző:Szöllősi János (biofizikus)
Cím:Fluorescence energy transfer measurements on cell surfaces : a critical comparison of steady-state fluorimetric and flow cytometric methods / János Szöllősi, Lajos Trón, Sándor Damjanovich, Stephen H. Helliwell, Donna Arndt-Jovin, Thomas M. Jovin
Dátum:1984
Megjegyzések:The energy transfer efficiency E was measured between fluorescein-conjugated concanavalin A (Con A) and rhodamine-conjugated Con A bound to homogeneous tissue culture cells, the HK22 murine lymphoma cell line. Results from a flow cytometric energy transfer method (FCET) and two different steady-state fluorimeter methods were compared. The data were found to be in close agreement after careful correction of the steady-state fluorimetric measurements for contributions from dissociating ligand. The biological variability of the individual cells with respect to E was calculated using an error propagation analysis and were found to be less than the variability in the absolute amount of ligand binding per cell. FCET has a number of advantages over the fluorimetric measurements using suspensions of cells: (1) relatively labile receptor-ligand complexes can be measured; (2) the analysis can be restricted to undamaged cells by gating the data collection on the light-scattering signals; (3) heterogeneous populations of cells with respect to donor and acceptor topology can be distinguished by the correlation of E with other cellular parameters derived from additional signals or combinations thereof; and (4) the dynamics of donor-acceptor redistribution on subpopulations can be measured.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
analysis
Animal
Cell Line
Cell Membrane
Concanavalin A
Energy Transfer
Flow Cytometry
Fluorescence
Lymphoma
Mathematics
methods
Mice
Mice,Inbred Strains
Microscopy,Fluorescence
pathology
physiology
physiopathology
Support,Non-U.S.Gov't
Megjelenés:Cytometry. - 5 : 2 (1984), p. 210-216. -
További szerzők:Trón Lajos (1941-) (biofizikus) Damjanovich Sándor (1936-2017) (biofizikus) Helliwell, Stephen H. Arndt-Jovin, Donna J. Jovin, Thomas M.
Internet cím:elektronikus változat
Borító:

11.

001-es BibID:BIBFORM006015
Első szerző:Szöllősi János (biofizikus)
Cím:Flow cytometric measurements of fluorescence energy transfer using single laser excitation / Szöllösi, J., Mátyus, L., Trón, L., Balázs, M., Ember, I., Fulwyler, M. J., Damjanovich, S.
Dátum:1987
Megjegyzések:Flow cytometric energy transfer (FCET) measurements between labeled specific sites of cell surface elements (Szollosi et al., Cytometry, 5:210-216, 1984) have been extended in a simplified form using a flow cytometer equipped with single excitation beam. This versatile and easily applicable method has several advantages over any nonflow cytometric (i.e., spectrofluorimetric) energy transfer measurements on cell surfaces: The labeled ligands can be applied in excess, without washing, thereby enabling the investigation of relatively labile receptor-ligand complexes. Contributions of signals from cell debris, from cell aggregates, or from nonviable cells can be avoided by gating the data collection on the light scatter signal. The heterogeneity of the cell population with respect to the proximity of the labeled binding sites can be studied. In the cases of homologous ligands or of ligands binding to the same molecule but at different epitopes, the determination of fluorescence resonance energy transfer efficiency values can be carried out on a cell-by-cell basis, offering data on intramolecular conformational changes. This modified FCET method enabled us to demonstrate the uniform density of glycoproteins, specific for Con A binding, in the plasma membrane of normal and Gross virus leukemic mouse cells of different sizes. The utility of this procedure has also been demonstrated by using the mean fluorescence intensities of the distribution curves in the calculation of the fluorescence energy transfer efficiency.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
analysis
Antigens,Surface
Binding Sites
diagnostic use
Energy Transfer
Epitopes
Flow Cytometry
Fluorescein
Fluoresceins
Fluorescence
Glycoproteins
instrumentation
Lasers
Ligands
Light
Lymphocytes
Lymphoma
Receptors,Concanavalin A
Rhodamines
Spectrometry,Fluorescence
Support,U.S.Gov't,Non-P.H.S.
Megjelenés:Cytometry. - 8 : 2 (1987), p. 120-128. -
További szerzők:Mátyus László (1956-) (biofizikus) Trón Lajos (1941-) (biofizikus) Balázs Margit (1952-) (sejtbiológus, molekuláris genetikus) Ember István Fulwyler, Mack J. Damjanovich Sándor (1936-2017) (biofizikus)
Internet cím:elektronikus változat
Intézményi repozitóriumban (DEA) tárolt változa
Borító:

12.

001-es BibID:BIBFORM004639
035-os BibID:(scopus)0032529665 (wos)000075434200001
Első szerző:Szöllősi János (biofizikus)
Cím:Application of fluorescence resonance energy transfer in the clinical laboratory : routine and research / Szollosi J., Damjanovich S., Matyus L.
Dátum:1998
ISSN:196-4763
Megjegyzések:Fluorescence resonance energy transfer (FRET) phenomenon has been applied to a variety of scientific challenges in the past. The potential utility of this biophysical tool will be revisited in the 21st century. The rapid digital signal processing in conjunction with personal computers and the wide use of multicolor laser technology in clinical flow cytometry opened an opportunity for multiplexed assay systems. The concept is very simple. Color-coded microspheres are used as solid-phase matrix for the detection of fluorescent labeled molecules. It is the homogeneous assay methodology in which solid-phase particles behave similarly to the dynamics of a liquid environment. This approach offers a rapid cost-effective technology that harnesses a wide variety of fluorochromes and lasers. With this microsphere technology, the potential applications for clinical flow cytometry in the future are enormous. This new approach of well-established clinically proven methods sets the stage to briefly review the theoretical and practical aspects of FRET technology. The review shows various applications of FRET in research and clinical laboratories. Combination of FRET with monoclonal antibodies resulted in a boom of structural analysis of proteins in solutions and also in biological membranes. Cell surface mapping of cluster of differentiation molecules on immunocompetent cells has gained more and more interest in the last decade. Several examples for biological applications are discussed in detail. FRET can also be used to improve the spectral characteristics of fluorescent dyes and dye combinations, such as the tandem dyes in flow and image cytometry and the FRET primers in DNA sequencing and polymerase chain reactions. The advantages and disadvantages of donor-acceptor dye combinations are evaluated. In addition, the sensitivity of FRET provides the basis for establishing fast, robust, and accurate enzyme assays and immunoassays. Benefits and limitations of FRET-based assays are thoroughly scrutinized. At the end of the paper we review the future of FRET methodology.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
analysis
Antibodies
Biophysics
Computers
Diagnostic Tests
Routine
Dna
Dyes
Energy Transfer
Flow Cytometry
Fluorescence
Fluorescent Dyes
Human
Hungary
Image Cytometry
Lasers
methods
Microspheres
Proteins
Research
Spectrometry
Support
Non-U.S.Gov't
Megjelenés:Cytometry. - 34 : 4 (1998), p. 159-179. -
További szerzők:Damjanovich Sándor (1936-2017) (biofizikus) Mátyus László (1956-) (biofizikus)
Internet cím:Intézményi repozitóriumban (DEA) tárolt változa
DOI
Borító:
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