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1.

001-es BibID:BIBFORM058444
Első szerző:Aszalos Adorján
Cím:An early cellular effect of Cyclosporin A is membrane potential change in human and mouse lymphocytes / A. Aszalos, S. Damjanovich, M. Balázs, L. Mátyus, L. Trón, S. M. Mulhern, A. D. Hess
Dátum:1985
Tárgyszavak:Orvostudományok Elméleti orvostudományok könyvfejezet
Membrane
Potential
Lymphocytes
Megjelenés:Recent Advances in Chemotherapy / ed. Ishigami J. - p. 2578-2579.
További szerzők:Damjanovich Sándor (1936-2017) (biofizikus) Balázs Margit (1952-) (sejtbiológus, molekuláris genetikus) Mátyus László (1956-) (biofizikus) Trón Lajos (1941-) (biofizikus) Mulhern, Sally Hess, A.
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2.

001-es BibID:BIBFORM045741
035-os BibID:(dekdb)9630543567
Első szerző:Aszalos Adorján
Cím:Depolymerization of microtubules increases motional freedom of lipid and protein probes in the cellular membrane and alters plasma membrane potential / Adorjan Aszalós, Michael M. Gottesman, Sandor Damjanovich
Dátum:1986
ISBN:9630543567
Tárgyszavak:Orvostudományok Elméleti orvostudományok könyvfejezet
Enzimek
Biofizika
Membránok (biológia)
Anyagcsere
Fehérjék
Nukleinsavak
Megjelenés:Dynamics of biochemical systems: lectures presented at the FEBS advanced course and round table discussion of the IUB interest group on kinetics and mechanisms of enzymes and metabolic networks: Debrecen, Hungary, 18-24 August 1985 / ed. by S. Damjanovich, T. Keleti, L. Trón. - p. 443-459. -
További szerzők:Gottesman, Michael M. Damjanovich Sándor (1936-2017) (biofizikus)
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3.

001-es BibID:BIBFORM005964
Első szerző:Aszalos Adorján
Cím:Depolymerization of microtubules alters membrane potential and affects the motional freedom of membrane proteins / Adorjan Aszalos, Sandor Damjanovich, Michael M. Gottesman
Dátum:1986
Megjegyzések:Two independent lines of evidence were obtained indicating that microtubule depolymerization affects the functions and the physical state of membranes in intact Chinese hamster ovary cells. The first type of evidence was obtained by using the dye dihexyloxacarbocyanine iodide to measure membrane potential before and after treatment with several microtubule active agents. Microtubule depolymerization resulted in a decrease in cell fluorescence, whereas stabilization of microtubules with taxol resulted in an increase in cell fluorescence. These effects of the drugs were due to their interactions with microtubules and not to direct effects of the drugs on the plasma membranes for the following reasons: effects were time dependent and required entry into the cells as indicated by the lack of fluorescence change in a multi-drug-resistant mutant that does not accumulate antimicrotubule drugs and a colcemid-resistant tubulin mutant did not show these effects on cell fluorescence. Evidence for altered motional freedom of membrane proteins in the plasma membrane was obtained by using electron spin resonance analysis of maleimide spin probe labeled cells. This study showed that depolymerization of microtubules results in increased motional freedom of maleimide-labeled sulfhydryl group containing proteins. Taken together, these data argue that microtubules function in mammalian cells to regulate the physical state of membranes and modulate membrane potential generated across cell membranes.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
analysis
Animal
Cell Line
Cell Membrane
Cricetulus
Electron Spin Resonance Spectroscopy
Female
Fluorescence
Hamsters
Kinetics
Membrane Potentials
Membrane Proteins
metabolism
methods
Microtubules
Ovary
physiology
ultrastructure
Megjelenés:Biochemistry. - 25 : 19 (1986), p. 5804-5809. -
További szerzők:Damjanovich Sándor (1936-2017) (biofizikus) Gottesman, Michael M.
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4.

001-es BibID:BIBFORM005963
Első szerző:Aszalos Adorján
Cím:Lymphocyte populations with different sensitivity to cyclosporin have different plasma membrane potentials / A. Aszalos, S. Damjanovich, P. Colombani, A. Hess
Dátum:1987
Megjegyzések:Cyclosporin A (CsA), a clinically potent immunosuppressive agent, shows preferential biologic activity against certain lymphocyte subsets. To investigate this activity, we used flow cytometry to separate two distinct lymphocyte populations from unfractionated human peripheral blood lymphocytes, based on their different binding affinity for a biologically active fluorescent, dansylated cyclosporin (dans-CsA) derivative. The separate lymphocyte populations demonstrated different resting plasma membrane potentials. The two lymphocyte populations also had different levels of interleukin-2 (IL-2) receptors and shifted plasma membrane potential differently upon treatment with cyclosporin and lymphokine IL-2. The cell population that bound more dans-CsA contained the cells which possessed IL-2 receptors and responded to CsA and IL-2 with changes in membrane potential. The cell population that did not effectively bind dans-CsA lacked IL-2 receptors and did not respond to CsA or IL-2 with immediate membrane potential changes. Furthermore, stimulation studies of these separated two lymphocyte populations with phytohemagglutinin in the presence and absence of CsA revealed that only the population which binds a low level of dans-CsA shows a marked difference in membrane potential between the cells stimulated in the presence and absence of CsA. We concluded that ion flux changes caused by CsA affect the activation process of naive lymphocytes but not that of already stimulated ones. It can also be postulated that the ion flux changing property of CsA could constitute its primary or main mode of action.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
blood
Cell Membrane
classification
Cyclosporins
drug effects
Flow Cytometry
Human
Interleukin-2
Lymphocytes
Membrane Potentials
pharmacology
physiology
Recombinant Proteins
Megjelenés:Jornal do Médico. - 18 : 5-6 (1987), p. 351-374. -
További szerzők:Damjanovich Sándor (1936-2017) (biofizikus) Colombani, P. Hess, A.
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5.

001-es BibID:BIBFORM035610
Első szerző:Damjanovich Sándor (biofizikus)
Cím:Mobility of HLA Class I antigen is influenced by anti-CD4 monoclonal antibody in lymphocyte membranes. A flow cytometric energy transfer, fluorescence photobleaching recovery and rotational relaxation study / Sandor Damjanovich; Laszlo Balkay; Laszlo Pohubi; Tamas Varhelyi; Laszlo Bene; Adorjan Aszalos; Laszlo Matyus; Lajos Tron
Dátum:1990
Tárgyszavak:Orvostudományok Elméleti orvostudományok előadáskivonat
Megjelenés:Time-Resolved Laser Spectroscopy in Biochemistry II. / szerk. Lakowicz, J. R. - p. 524-531.
További szerzők:Balkay László (1963-) (biofizikus) Pohubi László (1959-) (fizikus) Várhelyi Tamás Bene László (1963-) (biofizikus) Aszalos Adorján Mátyus László (1956-) (biofizikus) Trón Lajos (1941-) (biofizikus)
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6.

001-es BibID:BIBFORM005968
035-os BibID:(scopus)0023260317
Első szerző:Damjanovich Sándor (biofizikus)
Cím:Cyclosporin depolarizes human lymphocytes : earliest observed effect on cell metabolism / S. Damjanovich, A. Aszalos, S. A. Mulhern, J. Szollosi, M. Balazs, L. Tron, M. J. Fulwyler
Dátum:1987
Megjegyzések:Cyclosporin A (CsA) produced dose-dependent membrane depolarization of human peripheral blood lymphocytes. The phenomenon was investigated applying the membrane potential probe dihexyloxacarbocyanine iodide in a flow cytometer in combination with ionophores, hormones and monoclonal antibodies binding to different subclasses of lymphocytes and the anti-interleukin 2 receptor antibody. Human interferon-gamma abolished the depolarizing effect of cyclosporin on lymphocytes. Interleukin 2 caused depolarization and also enhanced the effect of CsA. OKT4 and OKT8 monoclonal antibodies slightly hindered depolarization by CsA while OKT3, OKT11 and OKIa1 antibodies had no such effect. Valinomycin decreased CsA's effect on the membrane potential while the ionophore A-23187 and ionomycin caused depolarizations that were additive with CsA's. CsA treatment released the isotope from 42K-loaded human lymphocytes in a dose-dependent fashion. CsA addition increased intracellular calcium content. CsA decreased the motional freedom of a spin probe in the membrane, but did not hinder the binding of fluoresceinated antibodies to the cell surface. These results suggest immediate alteration in membrane structure upon CsA treatment, causing potassium leakage and calcium ion uptake. These are the earliest detected effects of CsA on cells so far.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Antibodies,Monoclonal
blood
Calcium
Carbocyanines
Cell Membrane
classification
Cyclosporins
Cytoplasm
Dimethyl Sulfoxide
drug effects
Electron Spin Resonance Spectroscopy
Flow Cytometry
Human
immunology
Interferon Type II
Interleukin-2
Intracellular Membranes
Ion Channels
Ionomycin
Ionophores
Lymphocytes
Membrane Fluidity
Membrane Potentials
metabolism
methods
pharmacology
Potassium
Potassium Radioisotopes
Spectrometry,Fluorescence
ultrastructure
Valinomycin
Megjelenés:European Journal of Immunology. - 17 : 6 (1987), p. 763-768. -
További szerzők:Aszalos Adorján Mulhern, Sally Szöllősi János (1953-) (biofizikus) Balázs Margit (1952-) (sejtbiológus, molekuláris genetikus) Trón Lajos (1941-) (biofizikus) Fulwyler, Mack J.
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7.

001-es BibID:BIBFORM005967
Első szerző:Damjanovich Sándor (biofizikus)
Cím:Cytoplasmic membrane potential of mouse lymphocytes is decreased by cyclosporins / Damjanovich, S., Aszalos, A., Mulhern, S., Balazs, M., Matyus, L.
Dátum:1986
Megjegyzések:Membrane potential of mouse lymphocytes was investigated in the presence and absence of cyclosporin A (CsA) and cyclosporin G (CsG) by flow cytometry and fluorescence spectroscopy. A carbocyanine dye, dihexyloxacarbocyanine iodide [DIOC6(3)], was applied as a membrane potential probe. A dose-dependent decrease in the membrane potential of T and B lymphocytes was observed in the presence of CsA and CsG. However, pretreatment of lymphocytes with insulin reduced the effect of the cyclosporins. Mobile ionophores, such as valinomycin, ionomycin and A23187 were less effective in changing the membrane potential of lymphocytes in the presence of CsA. The channel forming ionophore, gramicidin or high extra-cellular potassium concentration (160 mM) strongly reduced the membrane potential regardless of the absence or presence the CsA. These observations suggest incorporation of CsA into the cytoplasmic membrane causing changes in ion fluxes. Other reported biochemical effects of CsA may be secondary to the observed membrane potential changes. The membrane potential change induced by CsA may have selective biological consequences in a certain subpopulation of lymphocytes.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Animal
antagonists and inhibitors
B-Lymphocytes
Cyclosporins
drug effects
Flow Cytometry
Fluorescence
Insulin
Ionomycin
Ionophores
Lymphocytes
Membrane Potentials
Mice
Mice,Inbred Strains
pharmacology
physiology
Potassium
Spectrometry,Fluorescence
Valinomycin
Megjelenés:Molecular Immunology. - 23 : 2 (1986), p. 175-180. -
További szerzők:Aszalos Adorján Mulhern, Sally Balázs Margit (1952-) (sejtbiológus, molekuláris genetikus) Mátyus László (1956-) (biofizikus)
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8.

001-es BibID:BIBFORM005975
Első szerző:Edidin, Michael
Cím:Lateral diffusion measurements give evidence for association of the Tac peptide of the IL-2 receptor with the T27 peptide in the plasma membrane of HUT-102-B2 T cells / M. Edidin, A. Aszalos, S. Damjanovich, T.A. Waldmann
Dátum:1988
Megjegyzések:Fluorescence photobleaching recovery measurements show that the F1-IgG-labeled Tac peptide of the IL-2R can diffuse in the plane of the membrane of HUT-102-B2 T lymphocytes, with a mean diffusion coefficient of 2 to 3 x 10(-10) cm2s-1. Although only a fraction (mean 37%) of the Tac peptides is mobile on any given cell, lateral diffusion of the Tac peptide can be measured in 94% of cells examined. In contrast, the 95-kDa peptide, T27, is 90 to 100% immobilized in cells labeled with OKT27. Immobilization of T27 also affects the lateral diffusion of the Tac peptide, because the Tac peptide is immobile in more than 30% of cells pretreated with OKT27 and then labeled with anti-Tac IgG. The effect is specific for OKT27 to the extent that pretreatment with an anti-HLA mAb does not immobilize the Tac peptide. It appears, then, that Tac and T27 peptide not only are in proximity on HUT-102-B2 lymphocyte membranes but also interact physically in situ.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Antibodies,Monoclonal
Antigens,CD27
Antigens,Surface
Cell Line
Diffusion
Fluorescence
HLA Antigens
Human
immunology
Interleukin-2
Lymphocytes
Membrane Proteins
metabolism
Peptides
physiology
Receptors,Immunologic
Receptors,Interleukin-2
Support,U.S.Gov't,P.H.S.
T-Lymphocytes
Megjelenés:The Journal of Immunology. - 141 : 4 (1988), p. 1206-1210. -
További szerzők:Aszalos Adorján Damjanovich Sándor (1936-2017) (biofizikus) Waldmann, Thomas A.
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9.

001-es BibID:BIBFORM005985
035-os BibID:PM:3011116
Első szerző:Mátyus László (biofizikus)
Cím:Cyclosporin A depolarizes cytoplasmic membrane potential and interacts with Ca2+ ionophores / László Mátyus, Margit Balázs, Adorján Aszalós, Sally Mulhern, Sándor Damjanovich
Dátum:1986
ISSN:0167-4889
Megjegyzések:Cytoplasmic membrane potential of mouse lymphocytes was determined with flow cytometry and fluorescence spectroscopy using 3,3'-dihexylcarbocyanine iodide (DiOC6(3)). The amount of this lipophilic cation incorporated into the cytoplasmic membrane is dependent upon the transmembrane potential, so the dye is suitable for continuous monitoring of this parameter, under controlled conditions. Membrane potential of the cells was decreased in the presence of cyclosporin A and cyclosporin G in a dose-dependent manner. However, the depolarization caused by Ca2+ ionophores, ionomycin and A23187, was reduced in the presence of cyclosporin A. Electron spin resonance spectroscopy with 5-doxylstearic acid as a probe indicated that cyclosporin A decreased the apparent motional freedom of membrane lipids. These data suggest incorporation of cyclosporin A into the cytoplasmic membrane, causing changes in ion fluxes. The membrane potential change induced by cyclosporin A may have selective biological consequences in certain subpopulations of lymphocytes.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Animal
Calcimycin
Carbocyanines
Cell Membrane
Cyclosporins
Dose-Response Relationship,Drug
drug effects
Drug Interactions
Electron Spin Resonance Spectroscopy
Ethers
Flow Cytometry
Fluorescence
Human
Insulin
Ionomycin
Ionophores
Lymphocyte Transformation
Lymphocytes
Membrane Lipids
Membrane Potentials
metabolism
Mice
Mice,Inbred Strains
pharmacology
Quinolines
ultrastructure
Megjelenés:Biochimica et Biophysica Acta (BBA). Molecular Cell Research. - 886 : 3 (1986), p. 353-360. -
További szerzők:Balázs Margit (1952-) (sejtbiológus, molekuláris genetikus) Aszalos Adorján Mulhern, Sally Damjanovich Sándor (1936-2017) (biofizikus)
Internet cím:Intézményi repozitóriumban (DEA) tárolt változa
DOI
Szerző által megadott URL
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10.

001-es BibID:BIBFORM046300
Első szerző:Panyi György (biofizikus)
Cím:Immunosuppressors inhibit voltage-gated potassium channels in human peripheral blood lymphocytes / Panyi G., Gaspar R., Krasznai Z., ter Horst J. J., Ameloot M., Aszalos A., Steels P., Damjanovich S.
Dátum:1996
ISSN:0006-291X
Megjegyzések:The effects of immunosuppressive agents on the potassium current of human peripheral blood lymphocytes have been studied using the whole-cell patch-clamp technique. Cyclosporin A (10 micrograms/ml), rapamycin (10 micrograms/ml) and FK-506 (2.5 micrograms/ml) reduced the peak K+ current by approximately 40, 30 and 40% of the control, respectively, without any change in the reversal potential of the current. The current inhibition was similar at all membrane potentials studied and was accompanied with an increase in the rate of K+ current inactivation. Membrane potential measurements in current-clamp showed a marked depolarization of the membrane (>10 mV) upon the addition of either immunosuppressor to the cells. Our findings revealed that the voltage-dependent potassium current in human peripheral blood lymphocytes is inhibited by Cyclosporin A and other immunosuppressors, resulting in a depolarized membrane potential.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:Biochemical and Biophysical Research Communications. - 221 : 2 (1996), p. 254-258. -
További szerzők:Gáspár Rezső (1944-) (biofizikus) Krasznai Zoltán (1950-) (biofizikus) ter Horst, Jan J. Ameloot, Marcel Aszalos Adorján Steels, Paul Damjanovich Sándor (1936-2017) (biofizikus)
Pályázati támogatás:1459
OTKA
1492
OTKA
6221
OTKA
E12533
OTKA
T14655
OTKA
F13335
OTKA
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11.

001-es BibID:BIBFORM006011
Első szerző:Szöllősi János (biofizikus)
Cím:Flow cytometric resonance energy transfer measurements support the association of a 95-kDa peptide termed T27 with the 55-kDa Tac peptide / J. Szöllösi, S. Damjanovich, C. K. Goldman, M. J. Fulwyler, A. A. Aszalos, G. Goldstein, P. Rao, M. A. Talle, T. A. Waldmann
Dátum:1987
Megjegyzések:Two monoclonal antibodies (OKT27 and OKT27b) have been produced that react with distinct epitopes of a 95-kDa peptide. The T27 antigen is widely distributed, being expressed on B lymphocytes, monocytes, and adult T-leukemic cells but not on polymorphonuclear leukocytes or platelets. There was a low level of T27 expression on resting T cells that increased on T-cell activation. In preliminary studies, the OKT27b antibody coprecipitated a 55-kDa peptide, as well as the 95-kDa peptide, from the radiolabeled cells of the HuT 102B2 cell line. Preclearance with anti-Tac, a monoclonal antibody to the 55-kDa peptide of the multichain interleukin 2 receptor, removed the 55-kDa but not the 95-kDa peptide from subsequent OKT27b immunoprecipitates of HuT 102B2 extracts, suggesting the possibility that the T27 peptide was associated with the Tac peptide. However, the precipitation of the p55 Tac peptide by OKT27b was quite inconsistent. Thus, additional information was sought using a flow cytometric energy transfer technique to provide a physical estimation of the proximity between the Tac and the T27 peptides. The flow cytometric version of the fluorescence resonance energy transfer technique permits the determination of inter- and intramolecular distances at 2- to 10-nm levels on a cell-by-cell basis. Using this approach, there was a mean energy transfer of 7.3% with HuT 102B2 cells when fluorescein isothiocyanate anti-Tac served as the donor and tetramethylrhodamine isothiocyanate OKT27 served as the acceptor. In contrast, there was no energy transfer in comparable studies observed when fluorescein anti-Tac and rhodamine anti-transferrin receptor antibodies were used. These observations support the conclusion that there is a close nonrandom proximity in HuT 102B2 cells between the 95-kDa peptide identified by the OKT27 monoclonal antibody and the p55 Tac peptide of the multichain interleukin 2 receptor.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Adult
analysis
Antibodies,Monoclonal
Antigens,CD27
Antigens,Neoplasm
Antigens,Surface
B-Lymphocytes
Biophysics
Cell Line
Energy Transfer
Epitopes
Flow Cytometry
Fluorescence
Human
Hungary
immunology
Interleukin-2
Lymphocytes
Monocytes
Peptides
Receptors,Immunologic
Receptors,Interleukin-2
Support,Non-U.S.Gov't
Support,U.S.Gov't,Non-P.H.S.
T-Lymphocytes
Tumor Cells,Cultured
Megjelenés:Proceedings of the National Academy of Sciences of the United States of America. - 84 : 20 (1987), p. 7246-7250. -
További szerzők:Damjanovich Sándor (1936-2017) (biofizikus) Goldman, Caroline K. Fulwyler, Mack J. Aszalos Adorján Goldstein, G. Rao, P. Talle, M. A. Waldmann, Thomas A.
Internet cím:elektronikus változat
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12.

001-es BibID:BIBFORM045740
035-os BibID:(dekdb)9630543567
Első szerző:Trón Lajos (biofizikus)
Cím:On the role of cell surface dynamics and transmembrane information transfer: cyclosporin A changes physical properties of cell membranes / L. Trón, S. Damjanovich, A. Aszalós, J. Szöllősi, Sally A. Mulhern, M. J. Fulwyler
Dátum:1986
ISBN:9630543567
Tárgyszavak:Orvostudományok Elméleti orvostudományok könyvfejezet
Enzimek
Biofizika
Membránok (biológia)
Anyagcsere
Fehérjék
Nukleinsavak
Megjelenés:Dynamics of biochemical systems: lectures presented at the FEBS advanced course and round table discussion of the IUB interest group on kinetics and mechanisms of enzymes and metabolic networks: Debrecen, Hungary, 18-24 August 1985 / ed. by S. Damjanovich, T. Keleti, L. Trón. - p. 417-441. -
További szerzők:Damjanovich Sándor (1936-2017) (biofizikus) Aszalos Adorján Szöllősi János (1953-) (biofizikus) Mulhern, Sally Fulwyler, Mack J.
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