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001-es BibID:	BIBFORM046086
Első szerző:	Dóczy-Bodnár Andrea (biofizikus)
Cím:	Class I HLA oligomerization at the surface of B cells is controlled by exogenous [béta]2-microglobulin : implications in activation of cytotoxic T lymphocytes / Bodnar A., Bacso Z., Jenei A., Jovin T. M., Edidin M., Damjanovich S., Matko J.
Dátum:	2003
ISSN:	1460-2377
Megjegyzések:	Submicroscopic molecular clusters (oligomers) of class I HLA have been detected by physical techniques [e.g. fluorescence resonance energy transfer (FRET) and single particle tracking of molecular diffusion] at the surface of various activated and transformed human cells, including B lymphocytes. Here, the sensitivity of this homotypic association to exogenous beta(2)-microglobulin (beta(2)m) and the role of free heavy chains (FHC) in class I HLA oligomerization were investigated on a B lymphoblastoid cell line, JY. Scanning near-field optical microscopy and FRET data both demonstrated that FHC and class I HLA heterodimers are co-clustered at the cell surface. Culturing the cells with excess beta(2)m resulted in a reduced co-clustering and decreased molecular homotypic association, as assessed by FRET. The decreased HLA clustering on JY target cells (antigen-presenting cells) was accompanied with their reduced susceptibility to specific lysis by allospecific CD8(+) cytotoxic T lymphocytes (CTL). JY B cells with reduced HLA clustering also provoked significantly weaker T cell activation signals, such as lower expression of CD69 activation marker and lower magnitude of TCR down-regulation, than did the untreated B cells. These results together suggest that the actual level of beta(2)m available at the cell surface can control CTL activation and the subsequent cytotoxic effector function through regulation of the homotypic HLA-I association. This might be especially important in some inflammatory and autoimmune diseases where elevated serum beta(2)m levels are reported.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:	International Immunology. - 15 : 3 (2003), p. 331-339. -
További szerzők:	Bacsó Zsolt (1963-) (biofizikus) Jenei Attila (1966-) (biofizikus) Jovin, Thomas M. Edidin, Michael Damjanovich Sándor (1936-2017) (biofizikus) Matkó János (1952-) (biológus)
Pályázati támogatás:	T034393 OTKA T030411 OTKA F034487 OTKA
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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001-es BibID:	BIBFORM005975
Első szerző:	Edidin, Michael
Cím:	Lateral diffusion measurements give evidence for association of the Tac peptide of the IL-2 receptor with the T27 peptide in the plasma membrane of HUT-102-B2 T cells / M. Edidin, A. Aszalos, S. Damjanovich, T.A. Waldmann
Dátum:	1988
Megjegyzések:	Fluorescence photobleaching recovery measurements show that the F1-IgG-labeled Tac peptide of the IL-2R can diffuse in the plane of the membrane of HUT-102-B2 T lymphocytes, with a mean diffusion coefficient of 2 to 3 x 10(-10) cm2s-1. Although only a fraction (mean 37%) of the Tac peptides is mobile on any given cell, lateral diffusion of the Tac peptide can be measured in 94% of cells examined. In contrast, the 95-kDa peptide, T27, is 90 to 100% immobilized in cells labeled with OKT27. Immobilization of T27 also affects the lateral diffusion of the Tac peptide, because the Tac peptide is immobile in more than 30% of cells pretreated with OKT27 and then labeled with anti-Tac IgG. The effect is specific for OKT27 to the extent that pretreatment with an anti-HLA mAb does not immobilize the Tac peptide. It appears, then, that Tac and T27 peptide not only are in proximity on HUT-102-B2 lymphocyte membranes but also interact physically in situ.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban Antibodies,Monoclonal Antigens,CD27 Antigens,Surface Cell Line Diffusion Fluorescence HLA Antigens Human immunology Interleukin-2 Lymphocytes Membrane Proteins metabolism Peptides physiology Receptors,Immunologic Receptors,Interleukin-2 Support,U.S.Gov't,P.H.S. T-Lymphocytes
Megjelenés:	The Journal of Immunology. - 141 : 4 (1988), p. 1206-1210. -
További szerzők:	Aszalos Adorján Damjanovich Sándor (1936-2017) (biofizikus) Waldmann, Thomas A.
Internet cím:	elektronikus változat
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