Találati lista | Tudóstér

001-es BibID:	BIBFORM006040
Első szerző:	Lakos Zsuzsa (biofizikus)
Cím:	The use of a new fluorescent viscosity indicator to study the relationship between transmembrane potential and membrane dynamics of lymphocytes / Lakos, Z., Volovyk, Z. M., Somogyi, B., Demchenko, O. P., Damjanovich, S.
Dátum:	1990
Megjegyzések:	A new fluorescent dye, polymethine derivative 4501 U, was applied to label the cytoplasmic membrane of mouse lymphocytes to investigate whether the membrane lipid dynamics is related to the transmembrane potential. The dye was found not to alter the cell viability. The dye is localized close to the external surface of the membrane, and its spectroscopic characteristics make it possible to obtain information on membrane fluidity using the intensity ratio of its two emission peaks. In contrast to recent data indicating that transmembrane potential alters the membrane lipid dynamics (as revealed by anisotropy studies with diphenyl hexatriene) 4501 U does not show such a relationship. The investigations presented support the hypothesis that the change in the transmembrane potential affects the dynamics/structure of the membrane region only at the interface between the two lipid layers.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban Animal Dyes Fluorescent Dyes Lymphocytes Membrane Fluidity Membrane Potentials metabolism Mice physiology Polymers Viscosity
Megjelenés:	Ukrainskiĭ biokhimicheskiĭ zhurnal. - 62 : 2 (1990), p. 107-110. -
További szerzők:	Volovyk, Z. M. Somogyi Béla (1945-2006) (biofizikus) Demchenko, O. P. Damjanovich Sándor (1936-2017) (biofizikus)
Internet cím:	elektronikus változat
Borító:
Saját polcon:

001-es BibID:	BIBFORM006039
035-os BibID:	PMID:2317496
Első szerző:	Lakos Zsuzsa (biofizikus)
Cím:	The effect of transmembrane potential on the dynamic behavior of cell membranes / Z. Lakos, B. Somogyi, M. Balazs, J. Matko, S. Damjanovich
Dátum:	1990
ISSN:	0005-2736
Megjegyzések:	The relationship between transmembrane potential and lipid dynamics in the cytoplasmic membrane of mouse thymus cells has been investigated. Changes of transmembrane potential was followed by measuring the fluorescence emission of the anionic dye, bis-(1,3-dibutylbarbiturate)trimethine oxonol (diBa-C4-(3)). Assessment of lipid fluidity was carried out applying three fluorescent lipid probes, 1-[4-(trimethylammonium)phenyl]-6-phenyl-1,3,5-hexatriene (TMA-DPH), 12-(9-anthroyloxy)stearic acid (12-AS) and 1,6-diphenyl-1,3,5-hexatriene (DPH) used to monitor different structural regions of the bilayer. The fluorescence anisotropy of these probes was measured as a function of temperature at two values of transmembrane potential. In the case of DPH it proved to depend on the membrane potential in the higher temperature range (above 28 degrees C), while no such a dependence could be observed for DPH below this temperature range and for TMA-DPH and 12-AS in between 20 and 37 degrees C. These data suggest that changes in transmembrane potential are accompanied with some local alteration in membrane lipid dynamics and/or structure
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban Cell membrane potential Membrane fluidity Lipid packing Fluorescence Fluorescence anisotropy
Megjelenés:	Biochimica et Biophysica Acta (BBA). Biomembranes. - 1023 : 1 (1990), p. 41-46. -
További szerzők:	Somogyi Béla (1945-2006) (biofizikus) Balázs Margit (1952-) (sejtbiológus, molekuláris genetikus) Matkó János (1952-) (biológus) Damjanovich Sándor (1936-2017) (biofizikus)
Internet cím:	Intézményi repozitóriumban (DEA) tárolt változat DOI Szerző által megadott URL
Borító:
Saját polcon: