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001-es BibID:BIBFORM006034
Első szerző:Gáspár Rezső (biofizikus)
Cím:Bretylium-induced voltage-gated sodium current in human lymphocytes / Rezső Gáspár, Zoltán Krasznai, Teréz Márián, Lajos Trón, Rina Recchioni, Marco Falasca, Fausto Moroni, Carlo Pieri, Sándor Damjanovich
Dátum:1992
ISSN:0167-4889
Megjegyzések:Using the whole-cell variation of the patch-clamp technique it has been determined that 0.25-3 mM bretylium tosylate (BT) exerts a repolarizing effect on partially depolarized human lymphocytes. The repolarizing effect was ouabain (40 microM)-sensitive, and was inhibited by the removal of external Na+ or by the Na(+)-channel-blocker amiloride (10-44 microM), but K(+)-channel-blockers 4-aminopyridine (0.1-5 mM) and quinine (100 microM) had no effect. The drug induced a sodium dependent, amiloride-sensitive transient inward current reaching its maximum value approx. 20-30 s after the administration of BT and lasting for 6-10 min. This current was activated by depolarization within 25 ms at around -42 mV, its inactivation took about 2 s and its reversal potential was +24 +/- 5 mV. An increase in the intracellular sodium concentration (1.8-3.2 mM) has been observed upon the addition of BT by monitoring the SBFI fluorescence of the dye-loaded cells. It has been shown that whole-cell K+ currents are significantly decreased by BT. The existence of voltage and ligand (BT)-gated sodium channels has been postulated in human lymphocytes. These channels are thought to participate in the initiation of membrane repolarization in human lymphocytes, and thereby influence mitogenic or antigen-induced cell-activation processes.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Bretylium
Ion channel
Patch-clamp
Human lymphocyte
Megjelenés:Biochimica et Biophysica Acta (BBA). Molecular Cell Research. - 1137 : 2 (1992), p. 143-147. -
További szerzők:Krasznai Zoltán (1950-) (biofizikus) Márián Teréz (1950-) (radiobiológus) Trón Lajos (1941-) (biofizikus) Recchioni, Rina Falasca, Marco Moroni, Fausto Pieri, Carlo Damjanovich Sándor (1936-2017) (biofizikus)
Internet cím:Intézményi repozitóriumban (DEA) tárolt változat
DOI
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2.

001-es BibID:BIBFORM006052
Első szerző:Pieri, Carlo
Cím:A sodium channel opener inhibits stimulation of human peripheral blood mononuclear cells / Pieri, C., Recchioni, R., Moroni, F., Marcheselli, F., Falasca, M., Krasznai, Z., Gaspar, R., Matyus, L., Damjanovich, S.
Dátum:1992
Megjegyzések:The role of membrane potential changes in T cell activation was studied on human peripheral blood lymphocytes stimulated with phytohemagglutinin. Addition of bretylium tosylate, a sodium channels opener, to PHA treated lymphocytes modified the membrane potential and consequently blocked cell activation in a dose-dependent fashion. BT was non-toxic even in long-term (72 hr) incubations. It was reversibly removable, and the removal restored the stimulatory effect of PHA. 3H-thymidine incorporation was blocked if BT was present during the first 20-24 hr of the mitogenic activation. The later BT was added after PHA, the less inhibition of proliferation was observed. BT hyperpolarized the lymphocytes also in the presence of PHA. BT hindered the depolarizing effect of high extracellular potassium concns. The sustained polarized state of the lymphocytes did not influence the intracellular calcium increase upon PHA treatment. IL-2 and transferrin receptor expression was not hindered by BT during PHA stimulation of lymphocytes. Addition of rIL-2 did not abolish the inhibitory effect of BT. According to cell-cycle analysis BT arrested the majority of the cells in G1 phase. It is suggested that cell activation demands the flexible maintenance of a relatively narrow membrane potential "window". Any sustained and significant hyper-, or depolarization, may dramatically decrease the effectivity of transmembrane signalling.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
analysis
blood
Bretylium Tosylate
Calcium
Cell Cycle
cytology
Dose-Response Relationship,Drug
drug effects
Flow Cytometry
Human
In Vitro
Interleukin-2
Lymphocyte Transformation
Lymphocytes
Membrane Potentials
pharmacology
physiology
Phytohemagglutinins
Potassium
Receptors,Transferrin
Research
Sodium
Sodium Channels
Support,Non-U.S.Gov't
T-Lymphocytes
Megjelenés:Molecular Immunology. - 29 : 4 (1992), p. 517-524. -
További szerzők:Recchioni, Rina Moroni, Fausto Marcheselli, Fiorella Falasca, Marco Krasznai Zoltán (1950-) (biofizikus) Gáspár Rezső (1944-) (biofizikus) Mátyus László (1956-) (biofizikus) Damjanovich Sándor (1936-2017) (biofizikus)
Internet cím:elektronikus változat
Intézményi repozitóriumban (DEA) tárolt változa
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