Találati lista | Tudóstér

001-es BibID:	BIBFORM116504
035-os BibID:	(cikkazonosító)112785 (scopus)85170688343 (wos)001081473700001
Első szerző:	Pevná, Viktória
Cím:	Effective transport of aggregated hypericin encapsulated in SBA-15 nanoporous silica particles for photodynamic therapy of cancer cells / Pevná Viktória, Zauska Lubos, Benziane Anass, Vámosi György, Girman Vladimír, Miklósová Monika, Zelenák Vladimír, Huntosová Veronika, Almási Miroslav
Dátum:	2023
ISSN:	1011-1344 1873-2682
Megjegyzések:	Photodynamic therapy (PDT) represents an interesting modality for the elimination of damaged biomaterials and cells. This treatment takes advantage of the photosensitizing properties of molecules that are active only when irradiated with light. In the present work, a dual property of hypericin, a hydrophobic molecule with high performance in photodiagnostics and photodynamic therapy, was exploited. The non-fluorescent and photodynamically inactive form of hypericin aggregates was loaded into the nanopores of SBA-15 silica particles. The synthesized particles were characterized by infrared spectroscopy, thermogravimetry, differential thermal analysis, small-angle X-ray scattering and transmission electron microscopy. Hypericin aggregates were confirmed by absorption spectra typical of aggregated hypericin and by its short fluorescence lifetime. Release of hypericin from the particles was observed toward serum proteins, mimicking physiological conditions. Temperature- and time-dependent uptake of hypericin by cancer cells showed gradual release of hypericin from the particles and active cellular transport by endocytosis. A closer examination of SBA-15-hypericin uptake by fluorescence lifetime imaging showed that aggregated hypericin molecules, characterized by a short fluorescence lifetime (?4 ns), were still present in the SBA-15 particles upon uptake by cells. However, monomerization of hypericin in cancer cells was observed by extending the hypericin fluorescence lifetime by ?8 ns, preferentially in lipid compartments and the plasma membrane. This suggests a promising prognosis for delayed biological activity of the entire cargo, which was confirmed by effective PDT in vitro. In summary, this work presents an approach for safe, inactive delivery of hypericin that is activated at the target site in cells and tissues.
Tárgyszavak:	Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk Nanoporous silica Hypericin Transport Cancer cells Fluorescence lifetime Photodynamic therapy
Megjelenés:	Journal Of Photochemistry And Photobiology B-Biology. - 247 (2023), p. 1-11. -
További szerzők:	Zauška, Ľuboš Benziane, Anass (1990-) (molekuláris biológus) Vámosi György (1967-) (biofizikus) Girman, Vladimír Miklóšová, Monika Zeleňák, Vladimír Huntošová, Veronika Almáši, Miroslav
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
Borító:
Saját polcon:

001-es BibID:	BIBFORM107128
035-os BibID:	(cikkazonosító)102896 (WoS)001009016200001 (Scopus)85147540608
Első szerző:	Rehó Bálint
Cím:	Agonist-controlled competition of RAR and VDR nuclear receptors for heterodimerization with RXR is manifested in their DNA-binding / Rehó Bálint, Fadel Lina, Brazda Peter, Benziane Anass, Hegedüs Éva, Sen Pialy, Gadella Theodorus W. Jr., Tóth Katalin, Nagy László, Vámosi György
Dátum:	2023
ISSN:	0021-9258 1083-351X
Megjegyzések:	We found previously that nuclear receptors (NRs) compete for heterodimerization with their common partner, retinoid X receptor (RXR), in a ligand-dependent manner. To investigate potential competition in their DNA binding, we monitored the mobility of retinoic acid receptor (RAR) and vitamin D receptor (VDR) in live cells by fluorescence correlation spectroscopy. First, specific agonist treatment and RXR coexpression additively increased RAR DNA binding, while both agonist and RXR were required for increased VDR DNA binding, indicating weaker DNA binding of the VDR/RXR dimer. Second, coexpression of RAR, VDR, and RXR resulted in competition for DNA binding. Without ligand, VDR reduced the DNA-bound fraction of RAR and vice versa, i.e., a fraction of RXR molecules was occupied by the competing partner. The DNA-bound fraction of either RAR or VDR was enhanced by its own and diminished by the competing NR`s agonist. When treated with both ligands, the DNA-bound fraction of RAR increased as much as due to its own agonist, whereas that of VDR increased less. RXR agonist also increased DNA binding of RAR at the expense of VDR. In summary, competition between RAR and VDR for RXR is also manifested in their DNA binding in an agonist-dependent manner: RAR dominates over VDR in the absence of agonist or with both agonists present. Thus, side effects of NR-ligand-based (retinoids, thiazolidinediones) therapies may be ameliorated by other NR ligands and be at least partly explained by reduced DNA binding due to competition. Our results also complement the model of NR action by involving competition both for RXR and for DNA sites.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk
Megjelenés:	Journal Of Biological Chemistry. - 299 : 2 (2023), p. 1-16. -
További szerzők:	Fadel, Lina (1988-) (gyógyszerész) Brázda Péter (1980-) (biológus, angol-magyar szakfordító) Benziane, Anass (1990-) (molekuláris biológus) Hegedűs Éva (1978-) (biofizikus) Sen, Pialy Gadella, Theodorus W. Jr. Tóth Katalin (biofizikus) Nagy László (1966-) (molekuláris sejtbiológus, biokémikus) Vámosi György (1967-) (biofizikus)
Pályázati támogatás:	GINOP-2.3.2-15-2016-00026 GINOP NN129371 OTKA ANN135107 OTKA Tempus Public Foundation: Stipendium Hungaricum scholarship Egyéb German Academic Exchange Service and the Tempus Public Foundation #273478 Egyéb
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
Borító:
Saját polcon: