Találati lista | Tudóstér

001-es BibID:	BIBFORM046289
Első szerző:	Bacsó Zsolt (biofizikus)
Cím:	A photobleaching energy transfer analysis of CD8MHC-I and LFA-1ICAM-1 interactions in CTL-target cell conjugates / Bacsó Zsolt, Bene László, Bodnár Andrea, Matkó János, Damjanovich Sándor
Dátum:	1996
ISSN:	0165-2478
Megjegyzések:	The photobleaching energy transfer (pbFRET) technique is a fluorescence method to measure proximity relationships between molecules, especially cell surface proteins, labeled with fluorophore-conjugated monoclonal antibodies, on a pixel-by-pixel base using digital imaging microscopy. This technique enables analysis of inter- and intramolecular proximities at cell surfaces at physiological conditions. We have developed a pbFRET approach to measure intercellular proximities in order to access spatial organization of interacting proteins in the contact region of two 'communicating' cells. Two examples, as possible application areas of this approach, are presented here: interaction between CD8 and MHC-I molecules in point contacts and interaction between LFA-1 and ICAM-1 molecules in focal contacts of CTL-target conjugates. The geometry of these protein contacts based on our resonance energy transfer (RET) data is consistent with the observed blocking effects of monoclonal antibodies (directed against the interacting proteins) on the cytolytic activity of CTLs and suggest a critical role for CD8beta-subunit in signal transmission in peripheral T-lymphocytes
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:	Immunology Letters. - 54 : 2-3 (1996), p. 151-156. -
További szerzők:	Bene László (1963-) (biofizikus) Dóczy-Bodnár Andrea (1970-) (biofizikus) Matkó János (1952-) (biológus) Damjanovich Sándor (1936-2017) (biofizikus)
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
Borító:
Saját polcon:

001-es BibID:	BIBFORM006064
035-os BibID:	WOS:A1996UY72200007
Első szerző:	Bacsó Zsolt (biofizikus)
Cím:	Changes in membrane potential of target cells promotes cytotoxic activity of effector T lymphocytes / Zsolt Bacsó, János Matkó, János Szöllősi, Rezső Gáspár, Sándor Damjanovich
Dátum:	1996
Megjegyzések:	The effector function of CD8+ lymphocytes depends on recognition by the TcR-CD3 complex of an oligopeptide presented by an MHC class I molecule on target cells. Recently it has been shown that MHC class I molecules change their conformation upon depolarization of human B lymphoblastoid JY cells. We studied here the effects of changes in membrane potential of target cells on the function of cytotoxic T lymphocytes (CTL). Selective alterations of plasma membrane potential of JY target cells were achieved by treatments with specific ionophore molecules as well as with Na(+)-K(+)-ATPase inhibitor, while the cytotoxic lymphocytes were not influenced. The plasma membrane was depolarized by gramicidin D and ouabain, while hyperpolarization was induced by valinomycin treatment. Alterations of the resting membrane potential of target cells in both direction resulted in an enhanced cytotoxic activity. The observed changes in cytolytic activities of cytotoxic T effectors may have a more general biological significance, namely apoptotic cells become depolarized after a given time, moreover neoplastic and virus infected cells also frequently show decreased membrane potential. A more efficient recognition of these cells by CTL is supposed to enhance the efficiency of their elimination, as well.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban Cytotoxicity,Immunologic Human Hungary immunology Lymphocytes Membrane Potentials Support,Non-U.S.Gov't T-Lymphocytes T-Lymphocytes,Cytotoxic Tumor Cells,Cultured Valinomycin egyetemen (Magyarországon) készült közlemény
Megjelenés:	Immunology Letters. - 51 : 3 (1996), p. 175-180. -
További szerzők:	Matkó János (1952-) (biológus) Szöllősi János (1953-) (biofizikus) Gáspár Rezső (1944-) (biofizikus) Damjanovich Sándor (1936-2017) (biofizikus)
Internet cím:	elektronikus változat DOI
Borító:
Saját polcon: