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001-es BibID:BIBFORM034925
Első szerző:Deckmyn, Hans
Cím:An echistatin-like Arg-Gly-Asp (RGD)-containing sequence in the heavy chain CDR3 of a murine monoclonal antibody that inhibits human platelet glycoprotein IIb/IIIa function / Deckmyn H., Stanssens P., Hoet B., Declerck P. J., Lauwereys M., Gansemans Y., Tornai I., Vermylen J.
Dátum:1994
ISSN:0007-1048
Megjegyzések:We describe the production and biochemical characterization of the first GPIIb/IIIa-inhibiting monoclonal antibody that contains an RGD sequence in the CDR3 region of the heavy chain. Monoclonal antibodies obtained by immunizing mice with human platelets were screened using consecutive ELISAs based on human platelets and immuno-affinity-purified glycoprotein (GP) IIb/IIIa coated on microtitre plates. Out of 30 monoclonal antibodies reacting with GPIIb/IIIa, one, MA-16N7C2, potently inhibited platelet aggregation induced by ADP, thrombin, arachidonic acid, collagen, U46619, adrenaline and platelet-activating factor, whereas ristocetin-induced aggregation was unaffected. MA-16N7C2 (IgG2a) bound approximately 4 times faster to activated than to resting platelets, with a Kdcalc of 6.6nM and of 17.5nM, respectively. Equilibrium binding studies to non-activated platelets showed a Kd of 18.2nM with 41 x 10(3) binding sites per platelet. The antibody recognized GPIIb/IIIa only as a Ca(2+)-dependent complex. MA-16N7C2 blocked fibrinogen and von Willebrand factor binding to GPIIb/IIIa in a competitive manner with a Ki of 8.5nM and 13.2nM, respectively. Sequence analysis revealed a RGD-containing sequence with homology to disintegrins, in the CDR3 region of the heavy chain. That this RGD-containing sequence could be involved in the interaction of the antibody to GPIIb/IIIa was finally indicated by showing that the binding is completely and competitively inhibited by echistatin.
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
antiplatelet
disintegrin
integrin
antibody sequence
külföldön készült közlemény
Megjelenés:British Journal Of Haematology. - 87 : 3 (1994), p. 562-571. -
További szerzők:Stanssens, Patrick Hoet, Bernard Declerck, Paul J. Lauwereys, Marc Gansemans, Yannick Tornai István (1954-) (belgyógyász, gasztroenterológus) Vermylen, Jozef
Internet cím:Intézményi repozitóriumban (DEA) tárolt változat
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2.

001-es BibID:BIBFORM034913
Első szerző:Tornai István (belgyógyász, gasztroenterológus)
Cím:Von Willebrand faktor-ellenes monoklonális antitestek kísérletes és gyakorlati alkalmazása / Tornai István, Arnout Jef, Deckmyn Hans, Declerck Paul, Peerlinck Kathelijne, Boda Zoltán, Vermylen Jos
Dátum:1994
Tárgyszavak:Orvostudományok Klinikai orvostudományok magyar nyelvű folyóiratközlemény hazai lapban
egyetemen (Magyarországon) készült közlemény
Megjelenés:Magyar Belorvosi Archívum. - 47 : 6 (1994), p. 441-447. -
További szerzők:Arnout, Jef Deckmyn, Hans Declerck, Paul J. Peerlinck, Kathelijne Boda Zoltán (1947-) (belgyógyász, haematologus, klinikai onkológus) Vermylen, Jozef
Internet cím:Intézményi repozitóriumban (DEA) tárolt változat
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3.

001-es BibID:BIBFORM034911
Első szerző:Tornai István (belgyógyász, gasztroenterológus)
Cím:Measurement of von Willebrand Factor Antigen in Plasma and Platelets with an Enzyme-Linked Immunosorbent Assay Based on Two Murine Monoclonal Antibodies / I. Tornai, P. J. Declerck, L. Smets, J. Arnout, H. Deckmyn, K. M. J. Caekebeke-Peerlinck, J. Vermylen
Dátum:1991
ISSN:0301-0147
Megjegyzések:Two murine monoclonal antibodies, raised against von Willebrand factor (vWF), were used to construct an enzyme-linked immunosorbent assay (ELISA), for quantitation of vWF antigen (vWFAg) in human plasma and platelets. This assay had a lower limit of sensitivity of 0.0001 IU/ml in buffer, and thus is one to two orders of magnitude more sensitive than other ELISA assays which have been reported. The intraassay, interassay and interdilution coefficients of variation were 4.1, 10.4 and 9.9%, respectively. In normal plasma (n = 20), the vWFAg level was 0.83 (range: 0.42-1.25) IU/ml. In normal washed platelets (n = 10), 0.35 (0.25-0.49) IU/10(9) platelets was found. In plasma obtained from various patient groups the following vWFAg levels (geometric mean and range) were observed: von Willebrand's disease (n = 19): 0.18 (0.02-0.77) IU/ml; patients with liver cirrhosis (n = 20): 3.73 (1.68-9.20) IU/ml; patients with pregnancy-induced hypertension (n = 20): 4.14 (2.28-7.44) IU/ml and patients with malignant disease (n = 10), 2.54 (1.51-5.60) IU/ml. A linear correlation was found between vWFAg levels measured with a polyclonal antibody based Laurell electroimmunoassay (r = 0.92, n = 58) or with a polyclonal antibody based ELISA (r = 0.94, n = 64). The present assay is based on stable and reproducible reagents and allows the specific measurement of vWFAg in plasma and in platelets. This assay may constitute a useful tool for the further investigation of clinical conditions associated with changes in vWFAg levels. In addition, its high sensitivity may facilitate a more detailed study of platelet vWFAg in normal and in pathological conditions.
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Monoclonal antibodies
külföldön készült közlemény
Immunosorbent assay
Plasma von Willebrand factor
Platelet von Willebrand factor
Megjelenés:Hemostasis. - 21 : 3 (1991), p. 125-134. -
További szerzők:Declerck, Paul J. Smets, L. Arnout, Jef Deckmyn, Hans Caekebeke-Peerlinck, K. M. J. Vermylen, Jozef
Internet cím:Intézményi repozitóriumban (DEA) tárolt változat
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