CCL

Összesen 3 találat.
#/oldal:
Részletezés:
Rendezés:

1.

001-es BibID:BIBFORM038657
Első szerző:Alving, Carl R.
Cím:HIV-1, lipid rafts, and antibodies to liposomes : implications for anti-viral-neutralizing antibodies (Review) / Alving C. R., Beck Z., Karasavvas N., Matyas G. R., Rao M.
Dátum:2006
ISSN:0968-7688
Megjegyzések:The human immunodeficiency virus type 1 (HIV-1) is an enveloped virus with a lipid bilayer that contains several glycoproteins that are anchored in, or closely associated with, the membrane surface. The envelope proteins have complex interactions with the lipids both on the host cells and on the target cells. The processes of budding from host cells and entry into target cells occur at sites on the plasma membrane, known as lipid rafts, that represent specialized regions that are rich in cholesterol and sphingolipids. Although the envelope glycoproteins are antigenic molecules that potentially might be used for development of broadly neutralizing antibodies in a vaccine to HIV-1, the development of such antibodies that have broad specificities against primary field isolates of virus has been largely thwarted to date by the ability of the envelope proteins to evade the immune system through various mechanisms. In this review, the interactions of HIV-1 with membrane lipids are summarized. Liposomes are commonly used as models for understanding interactions of proteins with membrane lipids; and liposomes have also been used both as carriers for vaccines, and as antigens for induction of antibodies to liposomal lipids. The possibility is proposed that liposomal lipids, or liposome-protein combinations, could be useful as antigens for inducing broadly neutralizing antibodies to HIV-1.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:Molecular Membrane Biology. - 23 : 6 (2006), p. 453-465. -
További szerzők:Beck Zoltán (1970-) (molekuláris biológus, mikrobiológus) Karasavvas, Nicos Matyas, Gary R. Rao, Mangala
Internet cím:Szerző által megadott URL
DOI
Intézményi repozitóriumban (DEA) tárolt változat
Borító:

2.

001-es BibID:BIBFORM002039
Első szerző:Beck Zoltán (molekuláris biológus, mikrobiológus)
Cím:Calcium modulation of monoclonal antibody binding to phosphatidylinositol phosphate / Zoltan Beck, Nicos Karasavvas, James Tong, Gary R. Matyas, Mangal Rao, Carl R. Alving
Dátum:2007
Megjegyzések:The binding characteristics of two monoclonal antibodies (mAb) to phosphatidylinositol-4-phosphate (PIP) were examined: a murine IgM mAb to PIP; and a human IgG mAb (4E10) that binds both to HIV-1 envelope protein and also to neutral and anionic phospholipids, including PIP. Binding of each mAb to pure PIP was inhibited by Ca2+ as determined by ELISA. When studied by surface plasmon resonance, liposomes containing PIP could be stripped (i.e., removed) by either Ca2+ or phosphorylated haptens after binding of the liposomes to the murine anti-PIP antibody attached to a BIAcore chip. In contrast, the binding of liposomal PIP to 4E10 was irreversible and could not be stripped. We therefore conclude that Ca2+ and phosphate can modulate the initial binding of both types of antibodies to PIP. However, 4E10 binds to liposomal PIP in a two-stage process involving first Ca2+-modulated binding to the PIP polar headgroup, followed by irreversible binding to liposomal hydrophobic groups.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
liposome
calcium
HIV-1
surface plasmon resonance
phosphoinositide
Megjelenés:Biochemical and Biophysical Research Communications. - 354 : 3 (2007), p. 747-751. -
További szerzők:Karasavvas, Nicos Tong, James Matyas, Gary R. Rao, Mangala Alving, Carl R.
Internet cím:elektronikus változat
DOI
elektronikus változat
Borító:

3.

001-es BibID:BIBFORM001904
Első szerző:Brown, Bruce K.
Cím:Monoclonal Antibodies to Phosphatidylinositol Phosphate Neutralize Human Immunodeficiency Virus Type 1 : role of Phosphate-Binding Subsites / Bruce K. Brown, Nicos Karasavvas, Zoltán Beck, Gary R. Matyas, Deborah L. Birx, Victoria R. Polonis, Carl R. Alving
Dátum:2007
Megjegyzések:Both a murine monoclonal antibody to phosphatidylinositol phosphate (PIP) and a human monoclonal antibody (4E10) that is known to have broadly neutralizing capabilities against primary isolates of human immunodeficiency virus type 1 (HIV-1) bound to PIP, as determined by enzyme-linked immunosorbent assay. Each of the antibodies had antigen subsite binding specificities in aqueous medium for small phosphate-containing molecules and for inositol. The anti-PIP monoclonal antibody inhibited infection by two HIV-1 primary isolates in neutralization assays employing primary human peripheral blood mononuclear cells. The data suggest that PIP or related lipids having free phosphates could serve as targets for the neutralization of HIV-1.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
HIV-1
Phosphatidylinositol Phosphate
Megjelenés:Journal of Virology. - 81 : 4 (2007), p. 2087-2091. -
További szerzők:Karasavvas, Nicos Matyas, Gary R. Birx, Deborah L. Polonis, Victoria R. Alving, Carl R. Beck Zoltán (1970-) (molekuláris biológus, mikrobiológus)
Internet cím:elektronikus változat
DOI
elektronikus változat
Borító:
Rekordok letöltése1