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001-es BibID:	BIBFORM005965
Első szerző:	Balázs Margit (sejtbiológus, molekuláris genetikus)
Cím:	Accessibility of cell surface thiols in human lymphocytes is altered by ionophores or OKT-3 antibody / Margit Balázs, János Matkó, János Szöllösi, László Mátyus, Mack J. Fulwyler, Sándor Damjanovich
Dátum:	1986
Megjegyzések:	The accessibility of cell surface sulfhydryl groups in human peripheral lymphocytes was investigated with 5,5'-dithiobis-(2-nitrobenzoic acid) in the presence and absence of ionophore antibiotics and the monoclonal antibody, OKT-3. Only a few accessible protein thiols have been found on the cells as demonstrated by labeling with a fluorescent non-penetrating thiol-marker, monobromotrimethyl-ammoniobimane and the subsequent gel electrophoretic analysis of the protein pattern. Difference spectrophotometric measurement of thiol-DTNB reaction revealed that ionophores altering the transmembrane potential induced an enhanced cell surface thiol-exposure on the minute time scale. The effect showed a dependence on the external concentration of the cations. The binding of monoclonal antibody, OKT-3, directed against T3 complexes, resulted in a similar, concentration-dependent increase of thiol-accessibility. These data are interpreted as early membrane-effects of ionophores and the specific antibody including changes in the conformational equilibrium or vertical displacements of certain membrane proteins. These events are likely to be coupled to the changes in the transmembrane potential of the lymphocytes.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk Ammonium Compounds analysis Antibiotics Antibodies,Monoclonal blood Cell Membrane Dithionitrobenzoic Acid drug effects Electrophoresis,Polyacrylamide Gel Human Ionophores Lymphocytes Membrane Proteins metabolism pharmacology Spectrometry,Fluorescence Sulfhydryl Compounds Support,Non-U.S.Gov't Support,U.S.Gov't,P.H.S.
Megjelenés:	Biochemical and Biophysical Research Communications. - 140 : 3 (1986), p. 999-1006. -
További szerzők:	Matkó János (1952-) (biológus) Szöllősi János (1953-) (biofizikus) Mátyus László (1956-) (biofizikus) Fulwyler, Mack J. Damjanovich Sándor (1936-2017) (biofizikus)
Internet cím:	elektronikus változat Intézményi repozitóriumban (DEA) tárolt változa DOI
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001-es BibID:	BIBFORM043991
Első szerző:	Pieri, Carlo
Cím:	Bretylium differentiates between distinct signal transducing pathways in human lymphocytes / Pieri, C., Bacso, Z., Recchioni, R., Moroni, F., Balazs, M., Gaspar, R., Damjanovich, S.
Dátum:	1993
ISSN:	0006-291X
Megjegyzések:	The selection of signal transducing pathways of T cells depends on the type of triggers. Antigens, antibodies or lectins induce the T cell receptor-CD3 operated pathway, and IL-2 transmits its activation signal via the IL-2 receptor. It has been demonstrated that bretylium, a quaternary ammonium ion, can significantly inhibit the first pathway at the same dose range that stimulates cell activation through the IL-2 receptor system. In the light of the different complexity of the two pathways at the plasma membrane level, and the non-toxic and reversible behavior of the drug, it is suggested that the bretylium induced sustained membrane hyperpolarization is responsible for the observation. This finding may open new possibilities in studying the mechanism of different signal transducing pathways.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:	Biochemical And Biophysical Research Communications. - 190 : 2 (1993), p. 654-659. -
További szerzők:	Bacsó Zsolt (1963-) (biofizikus) Recchioni, Rina Moroni, Fausto Balázs Margit (1952-) (sejtbiológus, molekuláris genetikus) Gáspár Rezső (1944-) (biofizikus) Damjanovich Sándor (1936-2017) (biofizikus)
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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