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001-es BibID:BIBFORM004663
035-os BibID:(scopus)0343962249 (wos)000087812700009
Első szerző:Gál István (sebész)
Cím:Protease-elicited TUNEL positivity of non-apoptotic fixed cells / Gal, I., Varga, T., Szilagyi, I., Balazs, M., Schlammadinger, J., Szabo, G.
Dátum:2000
Megjegyzések:The appearance of free DNA ends in the chromatin is usually considered an indication of advanced apoptosis. Unexpectedly, the nuclei of non-apoptotic cells derived from mouse thymuses could be specifically labeled by terminal transferase after proteinase K treatment of the fixed, cytocentrifuged samples. Artifactual mechanical or contaminating nucleolytic factors have been ruled out as players in the generation of free DNA ends. The phenomenon was detected in both formaldehyde- and ethanol-fixed specimens, in agarose-embedded fixed cells, and in chromatin spreads. By urea-agarose gel electrophoresis, the average single-strand size of the DNA molecules carrying the free ends was found between 50 and 250 kb. We suggest that ss discontinuities preexisting in the fixed normal cells are unmasked by protease treatment eliciting TUNEL (terminal transferase-mediated nick end-labeling) positivity.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Animals
Apoptosis
Artifacts
Biophysics
Cells
Chromatin
cytology
Dna
DNA Fragmentation
Electrophoresis, Agar Gel
Endopeptidase K
Formaldehyde
Hungary
In Situ Nick-End Labeling
metabolism
Mice
Mice, Inbred BALB C
physiology
Research
Support
Thymus Gland
Megjelenés:The Journal of Histochemistry and Cytochemistry. - 48 : 7 (2000), p. 963-970. -
További szerzők:Varga Tamás (1971-) (biológus) Szilágyi Ildikó (orvos) Balázs Margit (1952-) (sejtbiológus, molekuláris genetikus) Schlammadinger József (1938-) (kutató orvos) Szabó Gábor (1953-) (biofizikus)
Internet cím:DOI
elektronikus változat
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2.

001-es BibID:BIBFORM006042
035-os BibID:PMID:8419464
Első szerző:Márián Teréz (radiobiológus)
Cím:Hypo-osmotic shock induces an osmolality-dependent permeabilization and structural changes in the membrane of carp sperm / Teréz Márián, Zoltán Krasznai, László Balkay, Margit Balázs, Miklós Emri, László Bene, Lajos Trón
Dátum:1993
Megjegyzések:We carried out spectrofluorimetric and flow cytometric measurements to investigate the effect of hypo-osmotic shock on cell membranes of common carp sperm. The time course of the permeability of the sperm cell membrane, as monitored by DNA-related propidium iodide (PI) fluorescence, was followed for 30 min after dilution of semen in hypo-osmotic environments of different ionic strengths. Spectrofluorimetric measurements indicated a continuous increase in the total PI emission intensity of a sperm suspension. Cell-by-cell flow cytometric measurements suggested that the permeability changes were of the all-or-none type. The permeabilized fraction of cells in the individual samples was time and osmolality dependent. The number and percentage of cells in which DNA was stained by PI increased gradually over time and reached a steady-state plateau value after 5-15 min. This equilibrium fraction of cells with a PI-permeable cytoplasmic membrane displayed an inverse relationship with the osmolality of the diluent, having a near 100% value for fresh water and distilled water. Dilution of sperm in hypo-osmotic medium brought about a fast decrease in the forward light-scattering signal on a short time scale compared to the pre-steady-state time of the permeabilization. With the addition of extracellular Ca2+ (1.8 mM), restoration of the light scattering signal was observed. Permeabilization of the membrane and restoration of light scattering were not coincident in time. We propose a two-dimensional reorganization of the lipid structure as the underlying mechanism of the latter process.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Animal
Calcium
Calcium Chloride
Carps
Cell Membrane
Cell Membrane Permeability
Cells
Dna
drug effects
Flow Cytometry
Fluorescence
Hungary
Light
Male
Osmolar Concentration
Osmotic Pressure
pharmacology
physiology
Propidium
Semen
Spectrometry,Fluorescence
Spermatozoa
Support,Non-U.S.Gov't
ultrastructure
Water
egyetemen (Magyarországon) készült közlemény
Megjelenés:The Journal of Histochemistry and Cytochemistry. - 41 : 2 (1993), p. 291-297. -
További szerzők:Krasznai Zoltán (1950-) (biofizikus) Balkay László (1963-) (biofizikus) Balázs Margit (1952-) (sejtbiológus, molekuláris genetikus) Emri Miklós (1962-) (fizikus) Bene László (1963-) (biofizikus) Trón Lajos (1941-) (biofizikus)
Internet cím:elektronikus változat
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DOI
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