CCL

Összesen 7 találat.
#/oldal:
Részletezés:
Rendezés:

1.

001-es BibID:BIBFORM015664
Első szerző:Barok Márk (biofizikus)
Cím:Characterization of a novel, trastuzumab resistant human breast cancer cell line / Barok Mark, Balazs Margit, Lazar Viktoria, Rakosy Zsuzsa, Toth Eniko, Treszl Andrea, Vereb Gyorgy, Colbern G. T., Park J. W., Szollosi Janos
Dátum:2010
ISSN:1945-0508
Megjegyzések:HER2-positive breast cancers represent a distinct phenotype and are intrinsically more aggressive than HER2-negative tumors. Although HER2-targeted therapies have been rationally developed, resistance to these treatments represents a process understood poorly. There are few experimental models that allow studying the molecular mechanism of resistance. Our aim was to characterize a trastuzumab resistant breast cancer cell line (B585) that was established from an invasive ductal carcinoma. B585 grows only in immunodeficient mice as a xenograft. CGH and FISH were used to define cytogenetic alterations, gene-expression analysis and immunohistochemistry were applied to detect RNA and protein expression. By array-CGH focused amplifications were identified for C-MYC, EGFR, ErbB2, CCND1 and TOP2-A oncogenes. ErbB2 was co-amplified with TOP2-A. mRNA overexpression was detected for the amplified genes. ErbB2 protein was overexpressed and showed heterogeneous distribution. In summary, molecular cytogenetic analysis and expression profiling of B585 revealed several new alterations. Based on the experiments performed in SCID mice and the genotypic/phenotypic characteristics, this new in vivo breast cancer xenograft is a valuable model to investigate molecular mechanism of trastuzumab resistance
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
analysis
Animals
Antibodies
Antibodies,Monoclonal
Antineoplastic Agents
article
Biophysics
Breast Neoplasms
Carcinoma
Carcinoma,Ductal,Breast
Cell Line
Cell Line,Tumor
Comparative Genomic Hybridization
Disease Models,Animal
Drug Resistance,Neoplasm
drug therapy
EGFR
ErbB2
Female
Gene Expression
Gene Expression Profiling
genetics
Human
Humans
Hungary
Immunohistochemistry
In Situ Hybridization,Fluorescence
metabolism
Mice
Mice,Scid
Oncogenes
Phenotype
Receptor,erbB-2
Research
Research Support
rna
Support
therapeutic use
therapy
Trastuzumab resistance
OTKA::1
MAB::3.1
Megjelenés:Frontiers In Bioscience (elite edition). - 1 : 2 (2010), p. 627-640. -
További szerzők:Balázs Margit (1952-) (sejtbiológus, molekuláris genetikus) Lázár Viktória (1981-) (molekuláris biológus) Rákosy Zsuzsa (1978-) (sejtbiológus, molekuláris biológus, genetikus) Tóth Enikő Treszl Andrea (1974-) (molekuláris biológus) Vereb György (1965-) (biofizikus, orvos) Colbern, Gail T. Park, John W. Szöllősi János (1953-) (biofizikus)
Internet cím:Intézményi repozitóriumban (DEA) tárolt változat
Borító:

2.

001-es BibID:BIBFORM005174
035-os BibID:(scopus)38349044364 (wos)000253278400023
Első szerző:Barok Márk (biofizikus)
Cím:Trastuzumab decreases the number of circulating and disseminated tumor cells despite trastuzumab resistance of the primary tumor / Márk Barok, Margit Balázs, Péter Nagy, Zsuzsa Rákosy, Andrea Treszl, Enikő Tóth, István Juhász, John W. Park, Jorma Isola, György Vereb, János Szöllősi
Dátum:2008
ISSN:304-3835 (Print)
Megjegyzések:We have recently shown that despite of the fact that the ErbB2-positive JIMT-1 human breast cancer cells intrinsically resistant to trastuzumab in vitro, trastuzumab inhibited the outgrowth of early phase JIMT-1 xenografts in SCID mice via antibody-dependent cellular cytotoxicity (ADCC). Here we show that trastuzumab significantly reduces the number of circulating and disseminated tumor cells (CTCs and DTCs) in this xenograft model system at a time when the primary tumor is already unresponsive to trastuzumab. This observation suggests that ErbB2 positive CTCs and DTCs might be sensitive to trastuzumab-mediated ADCC even if when the primary tumor is already non-responsive. Thus, trastuzumab treatment might also be beneficial in the case of patients with breast cancer that is already trastuzumab resistant.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
analysis
Animals
antagonists and inhibitors
Antibodies
Antibodies, Monoclonal
Antigens
Antigens, CD45
Antineoplastic Agents
article
Biophysics
blood
Bone Marrow
Breast Neoplasms
Cell Line, Tumor
Cells
Chromosomes, Human,X
drug effects
Drug Resistance, Neoplasm
drug therapy
EGFR
Epidermal Growth Factor
ErbB2
Female
genetics
Histocompatibility Antigens
Histocompatibility Antigens Class I
Human
Humans
Hungary
Immunohistochemistry
immunology
In Situ Hybridization,Fluorescence
In Vitro
metabolism
Mice
Mice, Scid
mouse
Neoplasm Circulating Cells
Neoplasm Metastasis
pathology
pharmacology
Receptor, Epidermal Growth Factor
Research
Research Support
Support
therapeutic use
Time Factors
Trastuzumab resistance
Xenograft Model Antitumor Assays
egyetemen (Magyarországon) készült közlemény
Megjelenés:Cancer Letters. - 260 : 1-2 (2008), p. 198-208. -
További szerzők:Balázs Margit (1952-) (sejtbiológus, molekuláris genetikus) Nagy Péter (1971-) (biofizikus) Rákosy Zsuzsa (1978-) (sejtbiológus, molekuláris biológus, genetikus) Treszl Andrea (1974-) (molekuláris biológus) Tóth Enikő Juhász István (1956-) (bőrgyógyász, bőrsebész, kozmetológus, klinikai onkológus) Park, John W. Isola, Jorma Vereb György (1965-) (biofizikus, orvos) Szöllősi János (1953-) (biofizikus)
Internet cím:elektronikus változat
DOI
Borító:

3.

001-es BibID:BIBFORM001049
035-os BibID:(scopus)35448970162 (wos)000249715100018
Első szerző:Székvölgyi Lóránt (biofizikus, biokémikus, sejtbiológus)
Cím:Ribonucleoprotein-masked nicks at 50-kbp intervals in the eukaryotic genomic DNA / Lóránt Székvölgyi, Zsuzsa Rákosy, Bálint L. Bálint, Endre Kókai, László Imre, György Vereb, Zsolt Bacsó, Katalin Goda, Sándor Varga, Margit Balázs, Viktor Dombrádi, László Nagy, Gábor Szabó
Dátum:2007
Megjegyzések:By using a microscopic approach, field inversion single-cell gel electrophoresis, we show that preformed single-strand discontinuities are present in the chromatin of resting and proliferating mammalian and yeast cells. These single-strand breaks are primarily nicks positioned at approximately 50-kbp intervals throughout the entire genome that could be efficiently labeled in situ by DNA polymerase I holoenzyme but not by Klenow fragment and terminal transferase unless after ribonucleolytic treatments. The RNA molecules involved appear to comprise R-loops, recognized by the S9.6 RNA/DNA hybrid-specific antibody. By using the breakpoint cluster region of the Mixed Lineage Leukemia (MLL) gene as a model, we have found that the number of manifest nicks detected by FISH performed after field inversion single-cell gel electrophoresis depends on epigenetic context, but the difference between germ-line and translocated MLL alleles is abolished by protease treatment. Our data imply that the double-stranded genomic DNA is composed of contiguous rather than continuous single strands and reveal an aspect of higher-order chromatin organization with ribonucleoprotein-associated persistent nicks defining approximately 50-kbp domains.
Tárgyszavak:Orvostudományok Természettudományok Elméleti orvostudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
chromatin loop
RNA/DNA hybrid
translocation
Megjelenés:Proceedings of the National Academy of Sciences of the United States of America. - 104 : 38 (2007), p. 14964-14969. -
További szerzők:Rákosy Zsuzsa (1978-) (sejtbiológus, molekuláris biológus, genetikus) Bálint Bálint László (1971-) (kutató orvos) Kókai Endre (1971-) (biokémikus, biológus) Imre László (1979-) (biológus) Vereb György (1965-) (biofizikus, orvos) Bacsó Zsolt (1963-) (biofizikus) Goda Katalin (1969-) (biofizikus) Varga Sándor (1943-) (biofizikus) Balázs Margit (1952-) (sejtbiológus, molekuláris genetikus) Dombrádi Viktor (1953-) (biokémikus) Nagy László (1966-) (molekuláris sejtbiológus, biokémikus) Szabó Gábor (1953-) (biofizikus)
Internet cím:elektronikus változat
DOI
Borító:

4.

001-es BibID:BIBFORM033278
035-os BibID:PMID:8588941 WOS:A1995TF18900001
Első szerző:Vereb György (biofizikus, orvos)
Cím:Plasma-membrane-bound macromolecules are dynamically aggregated to form non-random codistribution patterns of selected functional elements. Do pattern recognition processes govern antigen presentation and intercellular interactions? / György Vereb, László Matyus, László Bene, György Panyi, Zsolt Bacsó, Margit Balázs, János Matkó, János Szöllősi, Rezső Gáspár, Sándor Damjanovich, Robert E. Dale, Carlo Pieri, Marcel Ameloot
Dátum:1995
Megjegyzések:Molecular recognition processes between cell surface elements are discussed with special reference to cell surface pattern formation of membrane-bound integral proteins. The existence, as detected by flow cytometric resonance energy transfer (Appendix), and significance of cell surface patterns involving the interleukin-2 receptor, the T-cell receptor-CD3 system, the intercellular adhesion molecule ICAM-1, and the major histocompatibility complex class I and class II molecules in the plasma membrane of lymphocytes are described. The modulation of antigen presentation by transmembrane potential changes is discussed, and a general role of transmembrane potential changes, and therefore of ion channel activities, adduced as one of the major regulatory mechanisms of cell-cell communication. A general role in the mediation and regulation of intercellular interactions is suggested for cell-surface macromolecular patterns. The dynamic pattern of protein and lipid molecules in the plasma membrane is generated by the genetic code, but has a remarkable flexibility and may be one of the major instruments of accommodation and recognition processes at the cellular level.
Tárgyszavak:Orvostudományok Egészségtudományok idegen nyelvű folyóiratközlemény külföldi lapban
cell surface
molecular pattern
energy transfer
fluorescence
flow cytometry
transmembrane potential
MHC
antigen presentation
intercellular communication
egyetemen (Magyarországon) készült közlemény
Megjelenés:Journal of Molecular Recognition. - 8 : 4 (1995), p. 237-246. -
További szerzők:Mátyus László (1956-) (biofizikus) Bene László (1963-) (biofizikus) Panyi György (1966-) (biofizikus) Bacsó Zsolt (1963-) (biofizikus) Balázs Margit (1952-) (sejtbiológus, molekuláris genetikus) Matkó János (1952-) (biológus) Szöllősi János (1953-) (biofizikus) Gáspár Rezső (1944-) (biofizikus) Damjanovich Sándor (1936-2017) (biofizikus) Dale, Robert E. Pieri, Carlo Ameloot, Marcel
Internet cím:DOI
Intézményi repozitóriumban (DEA) tárolt változat
Borító:

5.

001-es BibID:BIBFORM006057
Első szerző:Vereb György (biofizikus, orvos)
Cím:Effect of cyclosporin A on the membrane potential and Ca2+ level of human lymphoid cell lines and mouse thymocytes / György Vereb Jr., György Panyi, Margit Balázs, László Mátyus, János Matkó, Sándor Damjanovich
Dátum:1990
ISSN:0005-2728
Megjegyzések:The effect of the immunosuppressive cyclosporin A (CsA) on the cytosolic free Ca2+ concentration ([Ca2+]i) and membrane potential of human B and T lymphoblastoid cells and mouse thymocytes was studied in order to reveal some features of the early stage of drug-cell interaction. Cytosolic free Ca2+ concentration of the cells was measured by spectrofluorimetry using indo-1 and quin2 fluorescent calcium indicators. Membrane potential was monitored in a flow cytometer with oxonol dye. CsA applied at 2-20 micrograms/ml final concentrations caused a dose-dependent, rapid, transient rise of [Ca2+]i in all cell types. This effect could be blocked by chelating the extracellular Ca2+ with EGTA but was not sensitive to Ca2+ channel blockers verapamil and nifedipine or K+ channel blocker 4-aminopyridine. A possible explanation for the calcium mobilizing effect of CsA is an ionophore-like mode of action at the cell membrane level. Besides directly interfering with mitogenic signals, the elevation of [Ca2+]i could be responsible for an initial hyperpolarization observed in CsA-treated T lymphocytes. This hyperpolarization, however, was not detectable in B lymphoblastoid cells. A further difference between B and T cells was the diverse pattern of depolarization following CsA treatment. This variance in the behaviour of T and B lymphocytes and the diversity of membrane transport systems in its background could account for the different final outcome of the drug-cell interaction.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Cyclosporin A
Indo-1
Quin2
Membrane potential
Human lymphoid cell lines
Mouse thymocytes
Megjelenés:Biochimica et Biophysica Acta (BBA). Bioenergetics. - 1019 : 2 (1990), p. 159-165. -
További szerzők:Panyi György (1966-) (biofizikus) Balázs Margit (1952-) (sejtbiológus, molekuláris genetikus) Mátyus László (1956-) (biofizikus) Matkó János (1952-) (biológus) Damjanovich Sándor (1936-2017) (biofizikus)
Internet cím:Szerző által megadott URL
DOI
Intézményi repozitóriumban (DEA) tárolt változa
Borító:

6.

001-es BibID:BIBFORM006077
Első szerző:Vereb György (biofizikus, orvos)
Cím:Depletion of intracellular calcium stores facilitates the influx of extracellular calcium in platelet derived growth factor stimulated A172 glioblastoma cells / Vereb, G., Jr., Szollosi, J., Matyus, L., Balazs, M., Hyun, W. C., Feuerstein, B. G.
Dátum:1996
Megjegyzések:Calcium signaling in non-excitable cells is the consequence of calcium release from intracellular stores, at times followed by entry of extracellular calcium through the plasma membrane. To study whether entry of calcium depends upon the level of saturation of intracellular stores, we measured calcium channel opening in the plasma membrane of single confluent A172 glioblastoma cells stimulated with platelet derived growth factor (PDGF) and/or bradykinin (BK). We monitored the entry of extracellular calcium by measuring manganese quenching of Indo-1 fluorescence. PDGF raised intracellular calcium concentration ([Ca2+]i) after a dose-dependent delay (tdel) and then opened calcium channels after a dose-independent delay (tch). At higher doses (> 3 nM), BK increased [Ca2+]i after a tdel approximately 0 s, and tch decreased inversely with both dose and peak [Ca2+]i. Experiments with thapsigargin (TG), BK, and PDGF indicated that BK and PDGF share intracellular Ca2+ pools that are sensitive to TG. When these stores were depleted by treatment with BK and intracellular BAPTA, tdel did not change, but tch fell to almost 0 s in PDGF stimulated cells, indicating that depletion of calcium stores affects calcium channel opening in the plasma membrane. Our data support the capacitative model for calcium channel opening and the steady-state model describing quantal Ca2+ release from intracellular stores.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
antagonists and inhibitors
Biophysics
Bradykinin
Ca(2+)-Transporting ATPase
Calcium
Calcium Channel Blockers
Calcium Channels
Calcium Signaling
Dose-Response Relationship,Drug
drug effects
Fluorescence
Glioblastoma
Human
Hungary
Lanthanum
Manganese
metabolism
Neuroglia
pharmacology
Platelet-Derived Growth Factor
Signal Transduction
Support, Non-U.S. Gov't
Support, U.S.Gov't, Non-P.H.S.
Support, U.S.Gov't, P.H.S.
Terpenes
Thapsigargin
Tumor Cells,Cultured
Megjelenés:Cytometry. - 24 : 1 (1996), p. 64-73. -
További szerzők:Szöllősi János (1953-) (biofizikus) Mátyus László (1956-) (biofizikus) Balázs Margit (1952-) (sejtbiológus, molekuláris genetikus) Hyun, William C. Feuerstein, Burt G.
Internet cím:Intézményi repozitóriumban (DEA) tárolt változa
Borító:

7.

001-es BibID:BIBFORM004890
035-os BibID:(scopus)26444597596 (wos)000232299300017
Első szerző:Vereb György (biofizikus, orvos)
Cím:Biphasic calcium response of platelet-derived growth factor stimulated glioblastoma cells is a function of cell confluence / Vereb, G., Feuerstein, B. G., Hyun, W. C., Fulwyler, M. J., Balazs, M., Szollosi, J.
Dátum:2005
ISSN:1552-4922
Megjegyzések:Previous reports have linked the spiking or two-phased character of calcium transients evoked by platelet-derived growth factor (PDGF) to the position of cells in the cell cycle without regard to cell-cell contact and communication. Because cell confluence can regulate growth factor receptor expression and dephosphorylation, we investigated the effect of cell culture confluence and cell cycle on calcium responses of PDGF-BB-stimulated A172 glioblastoma cells. METHODS: Digital imaging cytometry was used to correlate the peak and duration of calcium response with bromodeoxyuridine positivity and DNA content and with culture confluence on a cell-by-cell basis. RESULTS: In serum-starved cultures, complete two-phase calcium signals and shorter, lower spikes occurred independent of cell cycle phase. However, the confluence of cell culture seemed essential for inducing a complete response because cells in sparse cultures exhibited mostly short spikes with lower peaks or no transients at all. CONCLUSION: Because cell confluence, by virtue of cell-cell contacts, is assumed to be an important regulator of proliferation, one is tempted to speculate that in transformed cells the ability to produce stronger growth signals upon reaching confluence and facing contact inhibition could provide a proliferative advantage.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
analysis
Biophysics
Bromodeoxyuridine
Calcium
Calcium Signaling
Cell Adhesion
Cell Culture
Cell Cycle
Cells
Cells,Cultured
Dna
drug effects
genetics
Glioblastoma
Hungary
metabolism
methods
pathology
pharmacology
Platelet-Derived Growth Factor
Research
Spectrometry,Fluorescence
Support
Megjelenés:Cytometry. Part A. - 67 : 2 (2005), p. 172-179. -
További szerzők:Feuerstein, Burt G. Hyun, William C. Fulwyler, Mack J. Balázs Margit (1952-) (sejtbiológus, molekuláris genetikus) Szöllősi János (1953-) (biofizikus)
Internet cím:elektronikus változat
DOI
Borító:
Rekordok letöltése1 
Corvina könyvtári katalógus v8.2.27 © 2023 Monguz kft. Minden jog fenntartva.