Találati lista | Tudóstér

001-es BibID:	BIBFORM004634
035-os BibID:	(scopus)0032101894 (wos)000073937700007
Első szerző:	Nagy Péter (biofizikus)
Cím:	EGF-induced redistribution of erbB2 on breast tumor cells : flow and image cytometric energy transfer measurements / Nagy, P., Bene, L., Balazs, M., Hyun, W. C., Lockett, S. J., Chiang, N. Y., Waldman, F., Feuerstein, B. G., Damjanovich, S., Szollosi, J.
Dátum:	1998
Megjegyzések:	erbB2, a member of the epidermal growth factor (EGF) receptor-type tyrosine kinase receptor family, is overexpressed in breast carcinomas with poor prognosis. We examined the cell surface association of this receptor with itself and with other cell surface proteins by the Forster-type fluorescence resonance energy transfer using whole antibodies and Fab fragments. We found that erbB2 molecules homoassociate in unstimulated SK-BR-3, BT474 and BT474-M (a metastatic version of the parent BT474 line) breast tumor cells, and that the interaction was enhanced by EGF treatment in suspensions of SK-BR-3 and BT474-M cells. BT474 cells (with low EGF receptor expression) and attached SK-BR-3 cells do not respond to EGF. Image microscopic energy transfer measurements found considerable pixel-by-pixel heterogeneity in the homoassociation state of erbB2. In accordance with the EGF-induced redistribution of erbB2, EGF receptor was found to be in close proximity to erbB2 in FRET measurements. By labeling different epitopes on erbB2 and the lipid bilayer, we were able to prepare an epitope map of erbB2 molecule. Our data suggest the existence of dynamic cell surface patterns of erbB2 and point to functions fulfilled by these molecular complexes.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk analysis biosynthesis Breast Neoplasms Carcinoma Cell Membrane chemistry drug effects Energy Transfer Epidermal Growth Factor Female Flow Cytometry Fluorescence Human Hungary metabolism methods Models Molecular pharmacology Receptor erbB-2 Receptors Transferrin Support Non-U.S.Gov't Tumor Cells,Cultured Squamous Cell ultrastructure
Megjelenés:	Cytometry. - 32 : 2 (1998), p. 120-131. -
További szerzők:	Bene László (1963-) (biofizikus) Balázs Margit (1952-) (sejtbiológus, molekuláris genetikus) Hyun, William C. Lockett, Steven J. Chiang, Nancy Y. Waldman, Frederick Feuerstein, Burt G. Damjanovich Sándor (1936-2017) (biofizikus) Szöllősi János (1953-) (biofizikus)
Internet cím:	elektronikus változat DOI
Borító:
Saját polcon:

001-es BibID:	BIBFORM006077
Első szerző:	Vereb György (biofizikus, orvos)
Cím:	Depletion of intracellular calcium stores facilitates the influx of extracellular calcium in platelet derived growth factor stimulated A172 glioblastoma cells / Vereb, G., Jr., Szollosi, J., Matyus, L., Balazs, M., Hyun, W. C., Feuerstein, B. G.
Dátum:	1996
Megjegyzések:	Calcium signaling in non-excitable cells is the consequence of calcium release from intracellular stores, at times followed by entry of extracellular calcium through the plasma membrane. To study whether entry of calcium depends upon the level of saturation of intracellular stores, we measured calcium channel opening in the plasma membrane of single confluent A172 glioblastoma cells stimulated with platelet derived growth factor (PDGF) and/or bradykinin (BK). We monitored the entry of extracellular calcium by measuring manganese quenching of Indo-1 fluorescence. PDGF raised intracellular calcium concentration ([Ca2+]i) after a dose-dependent delay (tdel) and then opened calcium channels after a dose-independent delay (tch). At higher doses (> 3 nM), BK increased [Ca2+]i after a tdel approximately 0 s, and tch decreased inversely with both dose and peak [Ca2+]i. Experiments with thapsigargin (TG), BK, and PDGF indicated that BK and PDGF share intracellular Ca2+ pools that are sensitive to TG. When these stores were depleted by treatment with BK and intracellular BAPTA, tdel did not change, but tch fell to almost 0 s in PDGF stimulated cells, indicating that depletion of calcium stores affects calcium channel opening in the plasma membrane. Our data support the capacitative model for calcium channel opening and the steady-state model describing quantal Ca2+ release from intracellular stores.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk antagonists and inhibitors Biophysics Bradykinin Ca(2+)-Transporting ATPase Calcium Calcium Channel Blockers Calcium Channels Calcium Signaling Dose-Response Relationship,Drug drug effects Fluorescence Glioblastoma Human Hungary Lanthanum Manganese metabolism Neuroglia pharmacology Platelet-Derived Growth Factor Signal Transduction Support, Non-U.S. Gov't Support, U.S.Gov't, Non-P.H.S. Support, U.S.Gov't, P.H.S. Terpenes Thapsigargin Tumor Cells,Cultured
Megjelenés:	Cytometry. - 24 : 1 (1996), p. 64-73. -
További szerzők:	Szöllősi János (1953-) (biofizikus) Mátyus László (1956-) (biofizikus) Balázs Margit (1952-) (sejtbiológus, molekuláris genetikus) Hyun, William C. Feuerstein, Burt G.
Internet cím:	Intézményi repozitóriumban (DEA) tárolt változa
Borító:
Saját polcon:

001-es BibID:	BIBFORM004890
035-os BibID:	(scopus)26444597596 (wos)000232299300017
Első szerző:	Vereb György (biofizikus, orvos)
Cím:	Biphasic calcium response of platelet-derived growth factor stimulated glioblastoma cells is a function of cell confluence / Vereb, G., Feuerstein, B. G., Hyun, W. C., Fulwyler, M. J., Balazs, M., Szollosi, J.
Dátum:	2005
ISSN:	1552-4922
Megjegyzések:	Previous reports have linked the spiking or two-phased character of calcium transients evoked by platelet-derived growth factor (PDGF) to the position of cells in the cell cycle without regard to cell-cell contact and communication. Because cell confluence can regulate growth factor receptor expression and dephosphorylation, we investigated the effect of cell culture confluence and cell cycle on calcium responses of PDGF-BB-stimulated A172 glioblastoma cells. METHODS: Digital imaging cytometry was used to correlate the peak and duration of calcium response with bromodeoxyuridine positivity and DNA content and with culture confluence on a cell-by-cell basis. RESULTS: In serum-starved cultures, complete two-phase calcium signals and shorter, lower spikes occurred independent of cell cycle phase. However, the confluence of cell culture seemed essential for inducing a complete response because cells in sparse cultures exhibited mostly short spikes with lower peaks or no transients at all. CONCLUSION: Because cell confluence, by virtue of cell-cell contacts, is assumed to be an important regulator of proliferation, one is tempted to speculate that in transformed cells the ability to produce stronger growth signals upon reaching confluence and facing contact inhibition could provide a proliferative advantage.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk analysis Biophysics Bromodeoxyuridine Calcium Calcium Signaling Cell Adhesion Cell Culture Cell Cycle Cells Cells,Cultured Dna drug effects genetics Glioblastoma Hungary metabolism methods pathology pharmacology Platelet-Derived Growth Factor Research Spectrometry,Fluorescence Support
Megjelenés:	Cytometry. Part A. - 67 : 2 (2005), p. 172-179. -
További szerzők:	Feuerstein, Burt G. Hyun, William C. Fulwyler, Mack J. Balázs Margit (1952-) (sejtbiológus, molekuláris genetikus) Szöllősi János (1953-) (biofizikus)
Internet cím:	elektronikus változat DOI
Borító:
Saját polcon: