CCL

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1.

001-es BibID:BIBFORM058444
Első szerző:Aszalos Adorján
Cím:An early cellular effect of Cyclosporin A is membrane potential change in human and mouse lymphocytes / A. Aszalos, S. Damjanovich, M. Balázs, L. Mátyus, L. Trón, S. M. Mulhern, A. D. Hess
Dátum:1985
Tárgyszavak:Orvostudományok Elméleti orvostudományok könyvfejezet
Membrane
Potential
Lymphocytes
Megjelenés:Recent Advances in Chemotherapy / ed. Ishigami J. - p. 2578-2579.
További szerzők:Damjanovich Sándor (1936-2017) (biofizikus) Balázs Margit (1952-) (sejtbiológus, molekuláris genetikus) Mátyus László (1956-) (biofizikus) Trón Lajos (1941-) (biofizikus) Mulhern, Sally Hess, A.
Internet cím:Intézményi repozitóriumban (DEA) tárolt változat
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2.

001-es BibID:BIBFORM005968
035-os BibID:(scopus)0023260317
Első szerző:Damjanovich Sándor (biofizikus)
Cím:Cyclosporin depolarizes human lymphocytes : earliest observed effect on cell metabolism / S. Damjanovich, A. Aszalos, S. A. Mulhern, J. Szollosi, M. Balazs, L. Tron, M. J. Fulwyler
Dátum:1987
Megjegyzések:Cyclosporin A (CsA) produced dose-dependent membrane depolarization of human peripheral blood lymphocytes. The phenomenon was investigated applying the membrane potential probe dihexyloxacarbocyanine iodide in a flow cytometer in combination with ionophores, hormones and monoclonal antibodies binding to different subclasses of lymphocytes and the anti-interleukin 2 receptor antibody. Human interferon-gamma abolished the depolarizing effect of cyclosporin on lymphocytes. Interleukin 2 caused depolarization and also enhanced the effect of CsA. OKT4 and OKT8 monoclonal antibodies slightly hindered depolarization by CsA while OKT3, OKT11 and OKIa1 antibodies had no such effect. Valinomycin decreased CsA's effect on the membrane potential while the ionophore A-23187 and ionomycin caused depolarizations that were additive with CsA's. CsA treatment released the isotope from 42K-loaded human lymphocytes in a dose-dependent fashion. CsA addition increased intracellular calcium content. CsA decreased the motional freedom of a spin probe in the membrane, but did not hinder the binding of fluoresceinated antibodies to the cell surface. These results suggest immediate alteration in membrane structure upon CsA treatment, causing potassium leakage and calcium ion uptake. These are the earliest detected effects of CsA on cells so far.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Antibodies,Monoclonal
blood
Calcium
Carbocyanines
Cell Membrane
classification
Cyclosporins
Cytoplasm
Dimethyl Sulfoxide
drug effects
Electron Spin Resonance Spectroscopy
Flow Cytometry
Human
immunology
Interferon Type II
Interleukin-2
Intracellular Membranes
Ion Channels
Ionomycin
Ionophores
Lymphocytes
Membrane Fluidity
Membrane Potentials
metabolism
methods
pharmacology
Potassium
Potassium Radioisotopes
Spectrometry,Fluorescence
ultrastructure
Valinomycin
Megjelenés:European Journal of Immunology. - 17 : 6 (1987), p. 763-768. -
További szerzők:Aszalos Adorján Mulhern, Sally Szöllősi János (1953-) (biofizikus) Balázs Margit (1952-) (sejtbiológus, molekuláris genetikus) Trón Lajos (1941-) (biofizikus) Fulwyler, Mack J.
Internet cím:elektronikus változat
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3.

001-es BibID:BIBFORM005967
Első szerző:Damjanovich Sándor (biofizikus)
Cím:Cytoplasmic membrane potential of mouse lymphocytes is decreased by cyclosporins / Damjanovich, S., Aszalos, A., Mulhern, S., Balazs, M., Matyus, L.
Dátum:1986
Megjegyzések:Membrane potential of mouse lymphocytes was investigated in the presence and absence of cyclosporin A (CsA) and cyclosporin G (CsG) by flow cytometry and fluorescence spectroscopy. A carbocyanine dye, dihexyloxacarbocyanine iodide [DIOC6(3)], was applied as a membrane potential probe. A dose-dependent decrease in the membrane potential of T and B lymphocytes was observed in the presence of CsA and CsG. However, pretreatment of lymphocytes with insulin reduced the effect of the cyclosporins. Mobile ionophores, such as valinomycin, ionomycin and A23187 were less effective in changing the membrane potential of lymphocytes in the presence of CsA. The channel forming ionophore, gramicidin or high extra-cellular potassium concentration (160 mM) strongly reduced the membrane potential regardless of the absence or presence the CsA. These observations suggest incorporation of CsA into the cytoplasmic membrane causing changes in ion fluxes. Other reported biochemical effects of CsA may be secondary to the observed membrane potential changes. The membrane potential change induced by CsA may have selective biological consequences in a certain subpopulation of lymphocytes.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Animal
antagonists and inhibitors
B-Lymphocytes
Cyclosporins
drug effects
Flow Cytometry
Fluorescence
Insulin
Ionomycin
Ionophores
Lymphocytes
Membrane Potentials
Mice
Mice,Inbred Strains
pharmacology
physiology
Potassium
Spectrometry,Fluorescence
Valinomycin
Megjelenés:Molecular Immunology. - 23 : 2 (1986), p. 175-180. -
További szerzők:Aszalos Adorján Mulhern, Sally Balázs Margit (1952-) (sejtbiológus, molekuláris genetikus) Mátyus László (1956-) (biofizikus)
Internet cím:elektronikus változat
Intézményi repozitóriumban (DEA) tárolt változa
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4.

001-es BibID:BIBFORM005985
035-os BibID:PM:3011116
Első szerző:Mátyus László (biofizikus)
Cím:Cyclosporin A depolarizes cytoplasmic membrane potential and interacts with Ca2+ ionophores / László Mátyus, Margit Balázs, Adorján Aszalós, Sally Mulhern, Sándor Damjanovich
Dátum:1986
ISSN:0167-4889
Megjegyzések:Cytoplasmic membrane potential of mouse lymphocytes was determined with flow cytometry and fluorescence spectroscopy using 3,3'-dihexylcarbocyanine iodide (DiOC6(3)). The amount of this lipophilic cation incorporated into the cytoplasmic membrane is dependent upon the transmembrane potential, so the dye is suitable for continuous monitoring of this parameter, under controlled conditions. Membrane potential of the cells was decreased in the presence of cyclosporin A and cyclosporin G in a dose-dependent manner. However, the depolarization caused by Ca2+ ionophores, ionomycin and A23187, was reduced in the presence of cyclosporin A. Electron spin resonance spectroscopy with 5-doxylstearic acid as a probe indicated that cyclosporin A decreased the apparent motional freedom of membrane lipids. These data suggest incorporation of cyclosporin A into the cytoplasmic membrane, causing changes in ion fluxes. The membrane potential change induced by cyclosporin A may have selective biological consequences in certain subpopulations of lymphocytes.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Animal
Calcimycin
Carbocyanines
Cell Membrane
Cyclosporins
Dose-Response Relationship,Drug
drug effects
Drug Interactions
Electron Spin Resonance Spectroscopy
Ethers
Flow Cytometry
Fluorescence
Human
Insulin
Ionomycin
Ionophores
Lymphocyte Transformation
Lymphocytes
Membrane Lipids
Membrane Potentials
metabolism
Mice
Mice,Inbred Strains
pharmacology
Quinolines
ultrastructure
Megjelenés:Biochimica et Biophysica Acta (BBA). Molecular Cell Research. - 886 : 3 (1986), p. 353-360. -
További szerzők:Balázs Margit (1952-) (sejtbiológus, molekuláris genetikus) Aszalos Adorján Mulhern, Sally Damjanovich Sándor (1936-2017) (biofizikus)
Internet cím:Intézményi repozitóriumban (DEA) tárolt változa
DOI
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5.

001-es BibID:BIBFORM006018
Első szerző:Trón Lajos (biofizikus)
Cím:On the biophysics of transmembrane signalling / L. Trón, A. Aszalos, M. Balázs, Sally A. Mulhern, J. Szöllösi, S. Damjanovich
Dátum:1988
Megjegyzések:Transmembrane signalling involves a number of physical translocations, changes in proximity of membrane elements like receptor subunits, or sequestration of proteins from the membrane. The monitoring of such changes with flow cytometric energy transfer revealed a new putative subunit of the IL-2 receptor and a possible intermolecular interaction between HLA class I and class II antigens. Lateral diffusion of the components of the multi-subunit IL-2 receptor was also followed. Changes in the intracellular pH were considered as a measure of efficient signal transfer in a number of cases. An overview and critical comparison of data is presented in the paper.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Biophysics
Cell Membrane
Diffusion
Energy Transfer
HLA Antigens
Human
Hungary
immunology
Interleukin-2
physiology
Receptors,Interleukin-2
Signal Transduction
Support,Non-U.S.Gov't
Megjelenés:Molecular Immunology. - 25 : 11 (1988), p. 1075-1080. -
További szerzők:Aszalos Adorján Balázs Margit (1952-) (sejtbiológus, molekuláris genetikus) Mulhern, Sally Szöllősi János (1953-) (biofizikus) Damjanovich Sándor (1936-2017) (biofizikus)
Internet cím:elektronikus változat
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