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001-es BibID:	BIBFORM033271
035-os BibID:	WOS:000168668400003
Első szerző:	Ádám Zsuzsa
Cím:	Liver metastatic ability of human melanoma cell line is associated with losses of chromosomes 4, 9p21-pter and 10p / Zsuzsanna Ádám, Róza Ádány, Andrea Ladányi, József Timár, Margit Balázs
Dátum:	2000
Megjegyzések:	Genetic changes underlying the aggressive progression of human cutaneous melanoma are not completely understood. In order to characterise genetic alterations associated with the metastatic behaviour of this neoplasm we used comparative genomic hybridisation (CGH) in combination with fluorescence in situ hybridisation (FISH) on an experimental metastatic model of three related human melanoma cell lines. Tumour lines were selected based on their various metastatic capacity to liver in immunosuppressed mice. The parental cell line (A2058) was a human amelanotic melanoma cell line, adaptation of this line to in vivo growth as xenograft the HT168 tumour and its cell line was established. After intrasplenic transplantation of HT168 cells into immunosuppressed mice, a highly metastatic variant (HT168-M1) was selected. Several chromosomal aberrations common to all three lines indicating common clonal origin, as well as additional non-shared chromosomal changes were found. The original cell line (A2058) exhibited the highest number of genetic changes. Chromosomal alterations present only in the highly metastatic line (HT168-M1) involved losses on chromosome 4, 9p21.3-pter and 10p. Chromosome copy number patterns and the nature of chromosome 4 loss were further investigated by FISH using different centromeric probes and a chromosome 4 painting probe. According to our CGH and FISH results we assume that alterations present only in the aggressive metastatic subline are associated with the increased - metastatic potential. Our observations further support the hypothesis, based on some recently published data, that certain (so far unidentified) suppressor genes having an important role in tumour progression are located on these chromosomes.
Tárgyszavak:	Orvostudományok Egészségtudományok idegen nyelvű folyóiratközlemény külföldi lapban experimental model FISH and CGH genetic alterations melanoma metastasis egyetemen (Magyarországon) készült közlemény
Megjelenés:	Clinical & experimental metastasis. - 18 : 4 (2000), p. 295-302. -
További szerzők:	Ladányi Andrea Timár József Ádány Róza (1952-) (megelőző orvostan és népegészségtan szakorvos) Balázs Margit (1952-) (sejtbiológus, molekuláris genetikus)
Internet cím:	DOI Intézményi repozitóriumban (DEA) tárolt változat
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001-es BibID:	BIBFORM039951
035-os BibID:	PMID:11514955
Első szerző:	Balázs Margit (sejtbiológus, molekuláris genetikus)
Cím:	Chromosomal imbalances in primary and metastatic melanomas revealed by comparative genomic hybridization / Margit Balázs, Zsuzsa Ádám, Andrea Treszl, Ágnes Bégány, János Hunyadi, Róza Ádány
Dátum:	2001
ISSN:	0196-4763
Megjegyzések:	Characteristic genetic changes underlying the metastatic progression of malignant melanoma is incompletely understood. The goal of our study was to explore specific chromosomal alterations associated with the aggressive behavior of this neoplasm. Comparative genomic hybridization was performed to screen and compare genomic imbalances present in primary and metastatic melanomas. Sixteen primary and 12 metastatic specimens were analyzed. We found that the pattern of chromosomal aberrations is similar in the two subgroups; however, alterations present only in primary and/or metastatic tumors were also discovered. The mean number of genetic changes was 6.3 (range 1-14) in primary and 7.8 (range 1-16) in metastatic lesions. Frequent losses involved 9p and 10q, whereas gains most often occurred at 1q, 6p, 7q, and 8q. Distinct, high-level amplifications were mapped to 1p12-p21 and 1p22-p31 in both tumor types. Amplification of 4q12-q13.1, 7q21.3-qter and 8q23-qter were detected only in primary tumors. The 20q13-qter amplicon was present in a metastatic tumor. The number of genetic alterations were significantly higher in primary tumors which developed metastases within one year after the surgery compared to tumors without metastasis during this time period. Fluorescence in situ hybridization with centromeric and locus-specific probes was applied to validate CGH results on a subset of tumors. Comparison of FISH and CGH data gave good correlation. The aggressive behavior of melanoma is associated with accumulation of multiple genetic alterations. Chromosome regions, which differ in the primary and metastatic lesions, may represent potential targets to identify metastases-related chromosomal alterations.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:	Cytometry. - 46 : 4 (2001), p. 222-232. -
További szerzők:	Ádám Zsuzsa Bégány Ágnes (1954-2011) (bőrgyógyász, kozmetológus, klinikai onkológus) Hunyadi János (1943-) (bőrgyógyász, kozmetológus, allergológus) Treszl Andrea (1974-) (molekuláris biológus) Ádány Róza (1952-) (megelőző orvostan és népegészségtan szakorvos)
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001-es BibID:	BIBFORM039706
Első szerző:	Balázs Margit (sejtbiológus, molekuláris genetikus)
Cím:	Involvement of chromosome losses in the progression and metastasis fo rmation of a human malginant melanoma / Balázs M., Ádám Zs., Bégány Á., Takuri Adel T., Ádány R.
Dátum:	1999
Megjegyzések:	To characterize the possible cytogenetic link between a primary tumor and its metastasis, interphase cytogenetic analysis was performed on tumor cells and cutaneous metastasis from a male patient with malignant melanoma by using fluorescence in situ hybridization. The numbers of distinct hybridization domains specific for ten different pericentromeric sequences were used as indicators of copy numbers of these chromosomes. In the primary tumor, the majority of cells had two copies of these chromosomes, but significant numbers of nuclei also were present with one and three copies. In addition, in almost all cells, both sex chromosomes were abnormal; nullisomy of the Y chromosome was associated with X disomy. The corresponding metastatic tumor cells were predominantly monosomic; only the distribution of chromosomes 11 and 7 was similar to the primary tumor. In the metastatic tumor, the sex chromosomes had a normal copy number; that is, one Y and one X were detected. These data indicate that both the initiation and the progression of this melanoma are associated with chromosome losses.
Tárgyszavak:	Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:	Cancer Genetics and Cytogenetics. - 109 : 2 (1999), p. 114-118. -
További szerzők:	Ádám Zsuzsa Bégány Ágnes (1954-2011) (bőrgyógyász, kozmetológus, klinikai onkológus) Takuri, Adel T. Ádány Róza (1952-) (megelőző orvostan és népegészségtan szakorvos)
Internet cím:	Intézményi repozitóriumban (DEA) tárolt változat DOI
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001-es BibID:	BIBFORM033272
035-os BibID:	WOS:000166207000017 PMID:11187904
Első szerző:	Nagy M.
Cím:	Genetic instability is associated with histological transformation of follicle center lymphoma / M. Nagy, M. Balázs, Z. Ádám, Z. Petkó, B. Timár, Z. Szereday, T. László, R. A. Warnke, A. Matolcsy
Dátum:	2000
Megjegyzések:	Follicle center lymphoma (FCL) is an indolent B cell non-Hodgkin's lymphoma (NHL) characterized genetically by the t(14;18) translocation. Histological transformation and clinical progression of FCLs are frequently associated with secondary genetic alterations at both nucleic acid and chromosomal levels. To determine the type and pattern of genomic instability occurring in histological transformation of FCLs and the role of DNA mismatch repair defects in this procedure, we have performed microsatellite analysis, comparative genomic hybridization (CGH) and mutational analysis of hMLH1 and hMSH2 genes on serial biopsy specimens from patients with FCL transformed to diffuse large cell lymphoma (DLCL). Paired biopsy samples of eight patients were analyzed for microsatellite instability and structural alterations for hMLH1 and hMSH2 genes, and tumor samples of five patients were subjected to CGH analysis. A high level of microsatellite instability was associated with histological transformation of two cases of FCL, but no mutations of the hMLH1 and hMSH2 genes were detected in any of the lymphoma samples. In the five cases subjected to CGH analysis, the histological transformation of FCLs was associated with genomic imbalances at 21 chromosomal regions. The genomic abnormalities found were rather heterogeneous and none of the genetic changes were overrepresented in the transformed DLCLs. These data suggest that histological transformation of FCLs to DLCL is frequently associated with genome wide instability at both nucleic acid and chromosomal levels, although mutations of the hMSH1 and hMLH2 genes are not involved in this process.
Tárgyszavak:	Orvostudományok Egészségtudományok idegen nyelvű folyóiratközlemény külföldi lapban follicular lymphoma genetic instability lymphoma transformation egyetemen (Magyarországon) készült közlemény
Megjelenés:	Leukemia. - 14 : 12 (2000), p. 2142-2148. -
További szerzők:	Ádám Zsuzsa Petkó Z. Timár Botond Szereday Zoltán László Terézia Warnke, R. A. Matolcsy András Balázs Margit (1952-) (sejtbiológus, molekuláris genetikus)
Internet cím:	DOI Intézményi repozitóriumban (DEA) tárolt változat
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