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001-es BibID:BIBFORM071356
Első szerző:Nagy Béla Jr. (labordiagnosztikai szakorvos)
Cím:Serum HE4 is a suitable inflammatory biomarker in cystic fibrosis / B. Nagy Jr., L. Fila, L. A. Clarke, Z. Fejes, P. Antal-Szalmás, J. Kappelmayer, M. D. Amaral, M. Macek Jr., I. Balogh
Dátum:2016
Megjegyzések:Serum HE4 is a suitable inflammatory biomarker in cystic fibrosisB. Nagy Jr1, L. Fila2, L.A. Clarke3, Z. Fejes1, P. Antal-Szalmás1, J. Kappelmayer1, M.D. Amaral3, M. Macek Jr4, I. Balogh11Department of Laboratory Medicine, Faculty of Medicine, University of Debrecen, Debrecen, Hungary, 2Department of Pulmonology,Charles University, 2nd Faculty of Medicine, Motol University Hospital, Prague, Czech Republic, 3University of Lisboa, Faculty of Sciences,BioISI-Biosystems & Integrative Sciences Institute, Lisboa, Portugal, 4Department of Biology and Medical Genetics, Motol UniversityHospital, 2nd Faculty of Medicine, Charles University, Prague, Czech RepublicIncreased human epididymis protein 4 (HE4) expression was previously observed in lung biopsy specimens of cystic fibrosis (CF). Accordingly,we presumed that serum HE4 concentrations were also elevated in CF, and might be used as a biomarker.In this study, 77 children with CF and 57 adult CF patients were enrolled. In parallel, 94 individuals with non-CF lung diseases, and 117normal controls without pulmonary disorders were analyzed. Serum HE4 was measured by chemiluminescent microparticle immunoassay(Architect?, Abbott). HE4 expression was further investigated via the quantification of HE4 mRNA using RT-qPCR in CF versus non-CF respiratoryepithelium biopsies. The expression of the potential regulator miR-140-5p was analyzed using an UPL-based RT-qPCR assay (Roche). Inaddition, HE4 was measured in the supernatants from unpolarized and polarized cystic fibrosis bronchial epithelial (CFBE) cells expressingWT- or F508del-CFTR.Serum HE4 levels were significantly elevated (P < 0.0001) in both CF children (99.5 [73.1-128.9] pmol/L) and CF adults (115.7 [77.8-148.7]pmol/L) compared to controls (36.3 [31.1-43.4] pmol/L). In contrast, abnormal but lower HE4 concentrations were found in cases of severebronchitis, asthma, pneumonia or bronchiectasis. HE4 concentrations positively correlated with disease severity and C-reactive protein concentrations in CF, while a significant inverse relationship was found between HE4 and the spirometric FEV1 value. Relative HE4 mRNAlevels were significantly augmented (P = 0.011) in the presence of decreased miR-140-5p expression (P = 0.020) in CF versus non-CF airwaybiopsies. Finally, 2-fold higher HE4 concentrations were measured in the supernatants of polarized F508del-CFTR CFBE cells compared toWT cells.In conclusion, serum HE4 positively correlates with the overall severity of CF and the degree of pulmonary dysfunction. Thus, HE4 maybe used as a novel inflammatory biomarker and for the treatment efficacy in this lung disease.
Tárgyszavak:Orvostudományok Klinikai orvostudományok idézhető absztrakt
Megjelenés:Clinical chemistry and laboratory medicine 54 : 10 (2016), p. 184-185. -
További szerzők:Fila, Libor Clarke, Luka A. Fejes Zsolt (1988-) (molekuláris biológus) Antal-Szalmás Péter (1968-) (laboratóriumi szakorvos) Kappelmayer János (1960-) (absztraktok) Amaral, Margarida D. Macek Jr., Milan Balogh István (1972-) (molekuláris biológus, genetikus)
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001-es BibID:BIBFORM071357
Első szerző:Nagy Gábor (laboratóriumi szakorvos, laboratóriumi hematológus és immunológus)
Cím:The role of automated microscopes and computer aided pattern recognition in autoantibody detection by indirect / G. Nagy, I. Csípő, J. Kappelmayer, P. Antal-Szalmás
Dátum:2016
Megjegyzések:The role of automated microscopes and computer aided pattern recognition in autoantibody detection by indirectimmunofluorescence assaysG. Nagy, I. Csípő, J. Kappelmayer, P. Antal-SzalmásDepartment of Laboratory Medicine, Faculty of Medicine, University of Debrecen, Debrecen, HungaryIndirect immunofluorescene assays (IFA) are versatile and sensitive solid phase tests for detecting autoantibodies needed to confirm the diagnosisof autoimmune diseases. Utilization of cells or tissue sections as the antigen source makes these assays capable of detecting antibodiesto delicate epitopes masked in other tests such as enzyme linked immunosorbent assay or immunoblot. However, conventional microscopicevaluation of the fluorescent patterns is time consuming and prone to transcription error.In our work we compared four automated fluorescence microscopes (Helios-Aesku Diagnostics, NovaView-Werfen Group, Europattern-Euroimmun, Image Navigator-Immunoconcept) regarding the number of available antigen substrates, specifications of the image capture andanalysis system, extent of the automation, patient safety and impact on the daily routine workflow.All four systems are able to detect antinuclear (ANA) and anti-cytoplasmic antibodies on HEp-2 epithelial cells (positive/negative discriminationonly) while Europattern and NovaView also help with the recognition of the most frequent ANA patterns. The sensitivity ofANA positive/negative discrimination is rather similar between the systems (around 95%), while the specificity varies between 85 to 95%.The capability for detection of anti-neutrophil cytoplasmic antibodies (ANCA) and image capture from other substrates like rat liver-kidneystomach(LKS) shows variability between the different analyzers. Similarly, the throughput, titer-estimation and physical parameters differsignificantly.Automation of the indirect immunofluorescence autoantibody testing is beneficial. In addition to slide processing, several fluorescencemicroscopes are available now which are able to digitize and archive IFA images. Their image analysis software helps to evaluate samples,making the indirect immunofluorescence method less laborious and error-prone.
Tárgyszavak:Orvostudományok Klinikai orvostudományok idézhető absztrakt
Megjelenés:Clinical Chemistry and Laboratory Medicine 54 : 10 (2016), p. 202. -
További szerzők:Csípő István (1953-) (vegyész) Kappelmayer János (1960-) (absztraktok) Antal-Szalmás Péter (1968-) (laboratóriumi szakorvos)
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001-es BibID:BIBFORM051862
Első szerző:Szánthó Eszter (laboratóriumi szakorvos jelölt)
Cím:Serum Thymidine Kinase Activity : analytical Performance, Age-Related Reference Ranges and Validation in Chronic Lymphocytic Leukemia / Eszter Szánthó, Harjit Pal Bhattoa, Mária Csobán, Péter Antal-Szalmás, Anikó Újfalusi, János Kappelmayer, Zsuzsanna Hevessy
Dátum:2014
ISSN:1932-6203
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:Plos One. - 9 : 3 (2014), p. e91647. -
További szerzők:Bhattoa Harjit Pal (1973-) (laboratóriumi szakorvos) Csobán Mária Antal-Szalmás Péter (1968-) (laboratóriumi szakorvos) Ujfalusi Anikó (1968-) (gyermekorvos, laboratóriumi szakorvos) Kappelmayer János (1960-) (absztraktok) Hevessy Zsuzsanna (1966-) (laboratóriumi szakorvos)
Internet cím:Intézményi repozitóriumban (DEA) tárolt változat
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