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1.

001-es BibID:BIBFORM098921
035-os BibID:(cikkazonosító)e2021ecc4
Első szerző:Collet, Claude
Cím:Insect elementary calcium release events measured in honeybee muscle cells / Claude Collet, Mercedes Charreton, Laszlo Szabo, Marianna Takacs, Laszlo Csernoch, Peter Szentesi
Dátum:2022
ISSN:0022-1295 1540-7748
Tárgyszavak:Orvostudományok Elméleti orvostudományok idézhető absztrakt
folyóiratcikk
Megjelenés:Journal Of General Physiology. - 154 : 9 (2022), p. e2021ecc4. -
További szerzők:Charreton, Mercédès Szabó László Takács Marianna (1991-) (állattenyésztés) Csernoch László (1961-) (élettanász) Szentesi Péter (1967-) (élettanász)
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2.

001-es BibID:BIBFORM040110
035-os BibID:PMID:8384243
Első szerző:Csernoch László (élettanász)
Cím:Microinjection of strong calcium buffers suppresses the peak of calcium release during depolarization in frog skeletal muscle fibers / László Csernoch, Vincent Jacquemond, Martin F. Schneider
Dátum:1993
ISSN:0022-1295
Megjegyzések:The effects of high intracellular concentrations of various calcium buffers on the myoplasmic calcium transient and on the rate of release of calcium (Rrel) from the sarcoplasmic reticulum (SR) were studied in voltage-clamped frog skeletal muscle fibers. The changes in intracellular calcium concentration (delta[Ca2+]) for 200-ms pulses to 0-20 mV were recorded before and after the injection of the calcium buffer and the underlying Rrel was calculated. If the buffer concentration after the injection was high, the initial rate of rise of the calcium transient was slower after injection than before and was followed by a slow increase of [Ca2+] that resembled a ramp. The increase in myoplasmic [Mg2+] that accompanies the calcium transient in control was suppressed after the injection and a slight decrease was observed instead. After the injection the buffer concentration in the voltage-clamped segment of the fiber decreased as the buffer diffused away toward the open ends. The calculated apparent diffusion coefficient for fura-2 (Dapp = 0.40 +/- 0.03 x 10(-6) cm2/s, mean +/- SEM, n = 6) suggests that approximately 65-70% of the indicator was bound to relatively immobile intracellular constituents. As the concentration of the injected buffer decreased, the above effects were reversed. The changes in delta[Ca2+] were underlined by characteristic modification of Rrel. The early peak component was suppressed or completely eliminated; thus, Rrel rose monotonically to a maintained steady level if corrected for depletion. If Rrel was expressed as percentage of SR calcium content, the steady level after injection did not differ significantly from that before. Control injections of anisidine, to the concentration that eliminated the peak of Rrel when high affinity buffers were used, had only a minor effect on Rrel, the peak was suppressed by 26 +/- 5% (mean +/- SE, n = 6), and the steady level remained unchanged. Thus, the peak component of Rrel is dependent on a rise in myoplasmic [Ca2+], consistent with calcium-induced calcium release, whereas the steady component of Rrel is independent of myoplasmic [Ca2+].
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
külföldön készült közlemény
Megjelenés:Journal of General Physiology. - 101 : 2 (1993), p. 297-333. -
További szerzők:Jacquemond, Vincent Schneider, Martin F.
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3.

001-es BibID:BIBFORM040106
Első szerző:Csernoch László (élettanász)
Cím:Interfering with calcium release suppresses I gamma, the "hump" component of intramembranous charge movement in skeletal muscle / L. Csernoch, G. Pizzaro, I. Uribe, M. Rodríguez, E. Ríos
Dátum:1991
ISSN:0022-1295
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
külföldön készült közlemény
Megjelenés:Journal of General Physiology. - 97 : 5 (1991), p. 845-884. -
További szerzők:Pizzaro, Gonzalo Uribe, I. Rodríguez, M. Ríos, Eduardo (1945-) (kutató)
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Intézményi repozitóriumban (DEA) tárolt változat
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4.

001-es BibID:BIBFORM040100
035-os BibID:PMID:3266232
Első szerző:Csernoch László (élettanász)
Cím:Differential effects of tetracaine on charge movements and Ca2+ signals in frog skeletal muscle / L. Csernoch, C. L.-H. Huang, G. Szűcs, L. Kovács
Dátum:1988
ISSN:0022-1295
Megjegyzések:The effects of tetracaine on charge movements and on antipyrylazo III signals monitoring intracellular delta [Ca2+] were compared in cut frog semitendinosus muscle fibers in a single vaseline gap-voltage clamp. Low tetracaine concentrations (25-40 microM) markedly reduced delta [Ca2+] signals and shifted the rheobase. However, they neither influenced charge movement nor that peak delta [Ca2+] value associated with the contractile threshold. Higher tetracaine concentrations (100-200 microM) partly inhibited charge movements in cut fibers. They separated a steeply voltage-sensitive charge, some of whose features resembled 'q gamma' reported in intact fibers, and whose movement preceded delta [Ca2+] signals at threshold. These findings: (a) directly confirm an earlier suggestion that tetracaine acts on steps in excitation-contraction coupling rather than myofilament activation; (b) show that tetracaine at low concentrations can directly interfere with sarcoplasmic reticular calcium release without modifying charge movement; (c) show that the tetracaine-sensitive charge, first found in intact fibers, also exists in cut fibers; and (d) make it unlikely that tetracaine-sensitive charge transfer is a consequence of Ca2+ release as suggested on earlier occasions.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
egyetemen (Magyarországon) készült közlemény
Megjelenés:Journal of General Physiology. - 92 : 5 (1988), p. 601-612. -
További szerzők:Huang, C. L.-H. Szűcs Géza (1948-) (élettanász) Kovács László (1939-) (élettanász)
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5.

001-es BibID:BIBFORM075067
035-os BibID:(WoS)000425926900009 (Scopus)85039962923 (PubMed)29229646
Első szerző:Launikonis, Bradley S.
Cím:NHE- and diffusion-dependent proton fluxes across the tubular system membranes of fast-twitch muscle fibers of the rat / Launikonis Bradley S., Cully Tanya R., Csernoch Laszlo, Stephenson D. George
Dátum:2018
ISSN:0022-1295 1540-7748
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Megjelenés:Journal Of General Physiology. - 150 : 1 (2018), p. 95-110. -
További szerzők:Cully, Tanya R. Csernoch László (1961-) (élettanász) Stephenson, D. George
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6.

001-es BibID:BIBFORM040105
035-os BibID:PMID:1650812
Első szerző:Pizzaro, Gonzalo
Cím:The relationship between Q gamma and Ca release from the sarcoplasmic reticulum in skeletal muscle / G. Pizarro, L. Csernoch, I. Uribe, M. Rodríguez, E. Ríos
Dátum:1991
ISSN:0022-1295
Megjegyzések:Asymmetric membrane currents and fluxes of Ca2+ release were determined in skeletal muscle fibers voltage clamped in a Vaseline-gap chamber. The conditioning pulse protocol 1 for suppressing Ca2+ release and the "hump" component of charge movement current (I gamma), described in the first paper of this series, was applied at different test pulse voltages. The amplitude of the current suppressed during the ON transient reached a maximum at slightly suprathreshold test voltages (-50 to -40 mV) and decayed at higher voltages. The component of charge movement current suppressed by 20 microM tetracaine also went through a maximum at low pulse voltages. This anomalous voltage dependence is thus a property of I gamma, defined by either the conditioning protocol or the tetracaine effect. A negative (inward-going) phase was often observed in the asymmetric current during the ON of depolarizing pulses. This inward phase was shown to be an intramembranous charge movement based on (a) its presence in the records of total membrane current, (b) its voltage dependence, with a maximum at slightly suprathreshold voltages, (c) its association with a "hump" in the asymmetric current, (d) its inhibition by interventions that reduce the "hump", (e) equality of ON and OFF areas in the records of asymmetric current presenting this inward phase, and (f) its kinetic relationship with the time derivative of Ca release flux. The nonmonotonic voltage dependence of the amplitude of the hump and the possibility of an inward phase of intramembranous charge movement are used as the main criteria in the quantitative testing of a specific model. According to this model, released Ca2+ binds to negatively charged sites on the myoplasmic face of the voltage sensor and increases the local transmembrane potential, thus driving additional charge movement (the hump). This model successfully predicts the anomalous voltage dependence and all the kinetic properties of I gamma described in the previous papers. It also accounts for the inward phase in total asymmetric current and in the current suppressed by protocol 1. According to this model, I gamma accompanies activating transitions at the same set of voltage sensors as I beta. Therefore it should open additional release channels, which in turn should cause more I gamma, providing a positive feedback mechanism in the regulation of calcium release.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
külföldön készült közlemény
Megjelenés:Journal of General Physiology. - 97 : 5 (1991), p. 913-947. -
További szerzők:Uribe, I. Rodríguez, M. Ríos, Eduardo (1945-) (kutató) Csernoch László (1961-) (élettanász)
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Intézményi repozitóriumban (DEA) tárolt változat
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7.

001-es BibID:BIBFORM096379
035-os BibID:(Scopus)85100993394
Első szerző:Sanchez, Colline
Cím:Detection of Ca2+ transients near ryanodine receptors by targeting fluorescent Ca2+ sensors to the triad / Colline Sanchez, Christine Berthier, Yves Tourneur, Laloé Monteiro, Bruno Allard, Laszlo Csernoch, Vincent Jacquemond
Dátum:2021
ISSN:0022-1295 1540-7748
Megjegyzések:In intact muscle fibers, functional properties of ryanodine receptor (RYR)?mediated sarcoplasmic reticulum (SR) Ca2+ release triggered by activation of the voltage sensor CaV1.1 have so far essentially been addressed with diffusible Ca2+-sensitive dyes. Here, we used a domain (T306) of the protein triadin to target the Ca2+-sensitive probe GCaMP6f to the junctional SR membrane, in the immediate vicinity of RYR channels, within the triad region. Fluorescence of untargeted GCaMP6f was distributed throughout the muscle fibers and experienced large Ca2+-dependent changes, with obvious kinetic delays, upon application of voltage-clamp depolarizing pulses. Conversely, T306-GCaMP6f localized to the triad and generated Ca2+-dependent fluorescence transients of lower amplitude and faster kinetics for low and intermediate levels of Ca2+ release than those of untargeted GCaMP6f. By contrast, model simulation of the spatial gradients of Ca2+ following Ca2+ release predicted limited kinetic differences under the assumptions that the two probes were present at the same concentration and suffered from identical kinetic limitations. At the spatial level, T306-GCaMP6f transients within distinct regions of a same fiber yielded a uniform time course, even at low levels of Ca2+ release activation. Similar observations were made using GCaMP6f fused to the ?1 auxiliary subunit of CaV1.1. Despite the probe's limitations, our results point out the remarkable synchronicity of voltage-dependent Ca2+ release activation and termination among individual triads and highlight the potential of the approach to visualize activation or closure of single groups of RYR channels. We anticipate targeting of improved Ca2+ sensors to the triad will provide illuminating insights into physiological normal RYR function and its dysfunction under stress or pathological conditions.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
SKELETAL-MUSCLE FIBERS
CALCIUM-RELEASE
SARCOPLASMIC-RETICULUM
MEMBRANE DEPOLARIZATION
VOLTAGE-DEPENDENCE
MYOPLASMIC CALCIUM
ELEMENTARY EVENTS
MOUSE
CALSEQUESTRIN
EXPRESSION
Megjelenés:Journal of General Physiology. - 153 : 4 (2021), p. e202012592. -
További szerzők:Berthier, Christine Tourneur, Yves Monteiro, Laloé Allard, Bruno Csernoch László (1961-) (élettanász) Jacquemond, Vincent
Pályázati támogatás:GINOP-2.3.2-15-2016-00044
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8.

001-es BibID:BIBFORM040083
Első szerző:Szentesi Péter (élettanász)
Cím:Effects of Dantrolene on Steps of Excitation-Contraction Coupling in Mammalian Skeletal Muscle Fibers / Péter Szentesi, Claude Collet, Sándor Sárközi, Csaba Szegedi, István Jona, Vincent Jacquemond, László Kovács, László Csernoch
Dátum:2001
ISSN:0022-1295
Megjegyzések:The effects of the muscle relaxant dantrolene on steps of excitation-contraction coupling were studied on fast twitch muscles of rodents. To identify the site of action of the drug, single fibers for voltage-clamp measurements, heavy SR vesicles for calcium efflux studies and solubilized SR calcium release channels/RYRs for lipid bilayer studies were isolated. Using the double Vaseline-gap or the silicone-clamp technique, dantrolene was found to suppress the depolarization-induced elevation in intracellular calcium concentration ([Ca2+]i) by inhibiting the release of calcium from the SR. The suppression of [Ca2+]i was dose-dependent, with no effect at or below 1 microM and a 53 +/- 8% (mean +/- SEM, n = 9, cut fibers) attenuation at 0 mV with 25 microM of extracellularly applied dantrolene. The drug was not found to be more effective if injected than if applied extracellularly. Calculating the SR calcium release revealed an equal suppression of the steady (53 +/- 8%) and of the early peak component (46 +/- 6%). The drug did not interfere with the activation of the voltage sensor in as much as the voltage dependence of both intramembrane charge movements and the L-type calcium currents (I(Ca)) were left, essentially, unaltered. However, the inactivation of I(Ca) was slowed fourfold, and the conductance was reduced from 200 +/- 16 to 143 +/- 8 SF(-1) (n = 10). Dantrolene was found to inhibit thymol-stimulated calcium efflux from heavy SR vesicles by 44 +/- 10% (n = 3) at 12 microM. On the other hand, dantrolene failed to affect the isolated RYR incorporated into lipid bilayers. The channel displayed a constant open probability for as long as 30-50 min after the application of the drug. These data locate the binding site for dantrolene to be on the SR membrane, but be distinct from the purified RYR itself.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:Journal Of General Physiology. - 118 : 4 (2001), p. 355-376. -
További szerzők:Collet, Claude Sárközi Sándor (1966-) (élettanász) Szegedi Csaba Jóna István (1948-) (élettanász, fizikus) Jacquemond, Vincent Kovács László (1939-) (élettanász) Csernoch László (1961-) (élettanász)
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Intézményi repozitóriumban (DEA) tárolt változat
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9.

001-es BibID:BIBFORM040108
035-os BibID:PMID:1940853
Első szerző:Szűcs Géza (élettanász)
Cím:Kinetic properties of intramembrane charge movement under depolarized conditions in frog skeletal muscle fibers / G. Szücs, Z. Papp, L. Csernoch, L. Kovács
Dátum:1991
ISSN:0022-1295
Megjegyzések:Intramembrane charge movement was measured on skeletal muscle fibers of the frog in a single Vaseline-gap voltage clamp. Charge movements determined both under polarized conditions (holding potential, VH = -100 mV; Qmax = 30.4 +/- 4.7 nC/micro(F), V = -44.4 mV, k = 14.1 mV; charge 1) and in depolarized states (VH = 0 mV; Qmax = 50.0 +/- 6.7 nC/micro(F), V = -109.1 mV, k = 26.6 mV; charge 2) had properties as reported earlier. Linear capacitance (LC) of the polarized fibers was increased by 8.8 +/- 4.0% compared with that of the depolarized fibers. Using control pulses measured under depolarized conditions to calculate charge 1, a minor change in the voltage dependence (to V = -44.6 mV and k = 14.5 mV) and a small increase in the maximal charge (to Qmax = 31.4 +/- 5.5 nC/micro(F] were observed. While in most cases charge 1 transients seemed to decay with a single exponential time course, charge 2 currents showed a characteristic biexponential behavior at membrane potentials between -90 and -180 mV. The voltage dependence of the rate constant of the slower component was fitted with a simple constant field diffusion model (alpha m = 28.7 s-1, V = -124.0 mV, and k = 15.6 mV). The midpoint voltage (V) was similar to that obtained from the Q-V fit of charge 2, while the steepness factor (k) resembled that of charge 1. This slow component could also be isolated using a stepped OFF protocol; that is, by hyperpolarizing the membrane to -190 mV for 200 ms and then coming back to 0 mV in two steps. The faster component was identified as an ionic current insensitive to 20 mM Co2+ but blocked by large hyperpolarizing pulses. These findings are consistent with the model implying that charge 1 and the slower component of charge 2 interconvert when the holding potential is changed. They also explain the difference previously found when comparing the steepness factors of the voltage dependence of charge 1 and charge 2.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
egyetemen (Magyarországon) készült közlemény
Megjelenés:Journal of General Physiology. - 98 : 2 (1991), p. 365-378. -
További szerzők:Papp Zoltán (1965-) (kardiológus, élettanász) Csernoch László (1961-) (élettanász) Kovács László (1939-) (élettanász)
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Intézményi repozitóriumban (DEA) tárolt változat
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10.

001-es BibID:BIBFORM032966
035-os BibID:PMID:1865176 WOS:A1991FM31500003
Első szerző:Szűcs Géza (élettanász)
Cím:Contraction threshold and the "hump" component of charge movement in frog skeletal muscle / G. Szűcs, L. Csernoch, J. Magyar, L. Kovács
Dátum:1991
Megjegyzések:The delayed component of intramembranous charge movement (hump, I gamma) was studied around the contraction threshold in cut skeletal muscle fibers of the frog (Rana esculenta) in a single Vaseline-gap voltage clamp. Charges (Q) were computed as 50-ms integrals of the ON (QON) and OFF (QOFF) of the asymmetric currents after subtracting a baseline. The hump appeared in parallel with an excess of QON over QOFF by approximately 2.5 nC/mu F. Caffeine (0.75 mM) not only shifted the contraction threshold but moved both the hump and the difference between the ON and OFF charges to more negative membrane potentials. When using 10-mV voltage steps on top of different prepulse levels, the delayed component, if present, was more readily observable. The voltage dependences of the ON and OFF charges measured with these pulses were clearly different: QON had a maximum at or slightly above the contraction threshold, while QOFF increased monotonically in the voltage range examined. Caffeine (0.75 mM) shifted this voltage dependence of QON toward more negative membrane potentials, while that of QOFF was hardly influenced. These results show that the delayed component of intramembranous charge movement either is much slower during the OFF than during the ON, or returns to the OFF position during the pulse. Tetracaine (25 microM) had similar effects on the charge movement currents, shifting the voltage dependence on the ON charge in parallel with the contraction threshold, but to more positive membrane potentials, and leaving QOFF essentially unchanged. The direct difference between the charge movement measured in the presence of caffeine and in control solution was either biphasic or resembled the component isolated by tetracaine, suggesting a common site of caffeine and tetracaine action. The results can be understood if the released Ca plays a direct role in the generation of the hump, as proposed in the first paper of this series.
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
egyetemen (Magyarországon) készült közlemény
Megjelenés:The Journal of General Physiology 97 : 5 (1991), p. 897-911. -
További szerzők:Csernoch László (1961-) (élettanász) Magyar János (1961-) (élettanász) Kovács László (1939-) (élettanász)
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Intézményi repozitóriumban (DEA) tárolt változat
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11.

001-es BibID:BIBFORM098925
035-os BibID:(cikkazonosító)e2021ecc33
Első szerző:Telek Andrea (élettanász)
Cím:Septin-7 is indispensable in skeletal muscle regeneration / Telek Andrea, Fodor Janos, Dobrosi Nora, Szabo Laszlo, Gönczi Monika, Dienes Beatrix, Csernoch Laszlo
Dátum:2022
ISSN:0022-1295 1540-7748
Tárgyszavak:Orvostudományok Elméleti orvostudományok idézhető absztrakt
folyóiratcikk
Megjelenés:Journal Of General Physiology. - 154 : 9 (2022), p. e2021ecc33. -
További szerzők:Fodor János (1973-) (élettanász, biotechnológus) Dobrosi Nóra (1981-) (molekuláris biológus) Szabó László Gönczi Mónika (1974-) (élettanász) Dienes Beatrix (1972-) (élettanász, molekuláris biológus) Csernoch László (1961-) (élettanász)
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Intézményi repozitóriumban (DEA) tárolt változat
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