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1.

001-es BibID:BIBFORM065847
Első szerző:Bodnár Dóra (molekuláris biológus)
Cím:Maurocalcin phosphorylated at threonin 26 maintains its activity on ryanodine receptor-mediated Ca2+ release in intact muscle fibers / Dóra Bodnár, Laszlo Csernoch, Vincent Jacquemond
Dátum:2016
ISSN:0027-8424
Tárgyszavak:Orvostudományok Elméleti orvostudományok levél
Megjelenés:Proceedings Of The National Academy Of Sciences Of The United States Of America 113 : 30 (2016), p. E4264-4265. -
További szerzők:Csernoch László (1961-) (élettanász) Jacquemond, Vincent
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2.

001-es BibID:BIBFORM040112
Első szerző:Collet, Claude
Cím:Effects of extracellular ATP on freshly isolated mouse skeletal muscle cells during pre-natal and post-natal development / Collet Claude, Strube Caroline, Csernoch Laszlo, Mallouk Nora, Ojeda Carlos, Allard Bruno, Jacquemond Vincent
Dátum:2002
ISSN:0031-6768
Megjegyzések:Extracellular adenosine 5'-triphosphate (ATP) has profound effects on membrane conductance and on the intracellular free [Ca(2+)] ([Ca(2+)](i)) in cultured skeletal muscle cells. The aim of the present study was to examine the occurrence and to characterize the properties of such responses during mammalian muscle development in vivo. The effect of ATP (0.2 mM) was tested on membrane current and [Ca(2+)](i) in freshly isolated pre- and post-natal mouse skeletal muscle cells. Pre-natal cells were from 14- to 19-day-old fetuses. In pre- and early post-natal cells, very small elevations of [Ca(2+)](i) (<50 nM) following ATP application could be detected with the fluorescent indicator fura-2. A clear subsarcolemmal rise in [Ca(2+)] was however associated to the presence of ATP, as demonstrated by increased activity of plasma membrane Ca(2+)-activated K(+) channels in cells bathed in a depolarizing, high-calcium-containing solution. In cells voltage-clamped at -80 mV in external Tyrode, ATP induced an inward current associated with an increased membrane conductance. The mean maximal amplitude of the ATP-induced current was -0.84 +/- 0.07 A/F ( n=39). The response to ATP was still present after birth, although its amplitude tended to decrease with post-natal development and was completely absent in muscle cells from 3- to 6-month-old mice. The ATP-induced current could be abolished reversibly by suramin. Our results suggest that, over the range of developmental stages examined, skeletal muscle cells display an ionotropic purinergic signalling pathway with functional properties qualitatively consistent with what is observed in cultured myotubes.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:Pflugers Archiv-European Journal Of Physiology. - 443 : 5-6 (2002), p. 771-778. -
További szerzők:Strube, Caroline Csernoch László (1961-) (élettanász) Mallouk, Nora Ojeda, Carlos Allard, Bruno Jacquemond, Vincent
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3.

001-es BibID:BIBFORM040113
Első szerző:Collet, Claude
Cím:Intramembrane charge movement and L-type calcium current in skeletal muscle fibers isolated from control and mdx mice / Collet, C., Csernoch, L., Jacquemond, V.
Dátum:2003
ISSN:0006-3495
Megjegyzések:Dystrophin-deficient muscle fibers from mdx mice are believed to suffer from increased calcium entry and elevated submembranous calcium level, the actual source and functional consequences of which remain obscure. Here we compare the properties of the dihydropyridine receptor as voltage sensor and calcium channel in control and mdx muscle fibers, using the silicone-voltage clamp technique. In control fibers charge movement followed a two-state Boltzmann distribution with values for maximal charge, midpoint voltage, and steepness of 23 +/- 2 nC/ micro F, -37 +/- 3 mV, and 13 +/- 1 mV (n = 7). Essentially identical values were obtained in mdx fibers and the time course of charge recovery from inactivation was also similar in the two populations (tau approximately 6 s). In control fibers the voltage dependence of the slow calcium current elicited by 100-ms-long pulses gave values for maximal conductance, apparent reversal potential, half-activation potential, and steepness factor of 156 +/- 15 S/F, 65.5 +/- 2.9 mV, -0.76 +/- 1.2 mV, and 6.2 +/- 0.5 mV (n = 17). In mdx fibers, the half-activation potential of the calcium current was slightly more negative (-6.2 +/- 1.2 mV, n = 16). Also, when using longer pulses, the time constant of calcium current decay was found to be significantly larger (by a factor of 1.5-2) in mdx than in control fibers. These changes in calcium current properties are unlikely to be primarily responsible for a dramatic alteration of intracellular calcium homeostasis. They may be speculated to result, at least in part, from remodeling of the submembranous cytoskeleton network due to the absence of dystrophin.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:Biophysical Journal. - 84 : 1 (2003), p. 251-265. -
További szerzők:Csernoch László (1961-) (élettanász) Jacquemond, Vincent
Internet cím:elektronikus változat
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4.

001-es BibID:BIBFORM040114
Első szerző:Collet, Claude
Cím:Calcium Signaling in Isolated Skeletal Muscle Fibers Investigated Under "Silicone Voltage-Clamp" Conditions / Collet Claude, Pouvreau Sandrine, Csernoch Laszlo, Allard Bruno, Jacquemond Vincent
Dátum:2004
ISSN:1085-9195
Megjegyzések:In skeletal muscle, release of calcium from the sarcoplasmic reticulum (SR) represents the major source of cytoplasmic Ca2+ elevation. SR calcium release is under the strict command of the membrane potential, which drives the interaction between the voltage sensors in the t-tubule membrane and the calcium-release channels. Either detection or control of the membrane voltage is thus essential when studying intracellular calcium signaling in an intact muscle fiber preparation. The silicone-clamp technique used in combination with intracellular calcium measurements represents an efficient tool for such studies. This article reviews some properties of the plasma membrane and intracellular signals measured with this methodology in mouse skeletal muscle fibers. Focus is given to the potency of this approach to investigate both fundamental aspects of excitation-contraction coupling and potential alterations of intracellular calcium handling in some muscle diseases.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:Cell Biochemistry And Biophysics. - 40 : 2 (2004), p. 225-236. -
További szerzők:Pouvreau, Sandrine Csernoch László (1961-) (élettanász) Allard, Bruno Jacquemond, Vincent
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5.

001-es BibID:BIBFORM061892
Első szerző:Csernoch László (élettanász)
Cím:Phosphoinositides in Ca2+ signaling and excitation-contraction coupling in skeletal muscle : an old player and newcomers / Laszlo Csernoch, Vincent Jacquemond
Dátum:2015
ISSN:0142-4319
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:Journal Of Muscle Research And Cell Motility 36 : 6 (2015), p. 491-499. -
További szerzők:Jacquemond, Vincent
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6.

001-es BibID:BIBFORM049815
Első szerző:Csernoch László (élettanász)
Cím:Voltage activated calcium release events in mouse skeletal muscle fibers / L. Csernoch, S. Pouvreau, V. Jacquemond
Dátum:2006
Tárgyszavak:Orvostudományok Elméleti orvostudományok idézhető absztrakt
Megjelenés:Biophysical Journal. - 90 (2006), p. 326a. -
További szerzők:Pouvreau, Sandrine Jacquemond, Vincent
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7.

001-es BibID:BIBFORM049737
Első szerző:Csernoch László (élettanász)
Cím:The effect of BAPTA type calcium buffers on the calcium release in frog skeletal muscle fibers / L. Csernoch, V. Jacquemond, J. P. Y. Kao, M. F. Schneider
Dátum:1992
Tárgyszavak:Orvostudományok Elméleti orvostudományok idézhető absztrakt
Megjelenés:Biophysical Journal. - 61 (1992), p. 23a. -
További szerzők:Jacquemond, Vincent Kao, J. P. Y. Schneider, Martin F.
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8.

001-es BibID:BIBFORM050030
Első szerző:Csernoch László (élettanász)
Cím:Peak calcium release suppressed by high affinity calcium buffers applied from the cut ends of frog skeletal muscle fibers / L. Csernoch, V. Jacquemond, M. F. Schneider
Dátum:1993
ISSN:0006-3495
Tárgyszavak:Orvostudományok Elméleti orvostudományok idézhető absztrakt
Megjelenés:Biophysical Journal. - 64 (1993), p. 37a. -
További szerzők:Jacquemond, Vincent Schneider, Martin F.
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9.

001-es BibID:BIBFORM050032
Első szerző:Csernoch László (élettanász)
Cím:Measurements of free intracellular magnesium concentration ([Mg++]i) using the fluorescent indicator magindo-1 in isolated mouse skeletal muscle fibers / L. Csernoch, J. C. Bernengo, V. Jacquemond
Dátum:1996
ISSN:0006-3495
Tárgyszavak:Orvostudományok Elméleti orvostudományok idézhető absztrakt
Megjelenés:Biophysical Journal. - 70 (1996), p. A169. -
További szerzők:Bernengo, Jean Claude Jacquemond, Vincent
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10.

001-es BibID:BIBFORM040110
035-os BibID:PMID:8384243
Első szerző:Csernoch László (élettanász)
Cím:Microinjection of strong calcium buffers suppresses the peak of calcium release during depolarization in frog skeletal muscle fibers / László Csernoch, Vincent Jacquemond, Martin F. Schneider
Dátum:1993
ISSN:0022-1295
Megjegyzések:The effects of high intracellular concentrations of various calcium buffers on the myoplasmic calcium transient and on the rate of release of calcium (Rrel) from the sarcoplasmic reticulum (SR) were studied in voltage-clamped frog skeletal muscle fibers. The changes in intracellular calcium concentration (delta[Ca2+]) for 200-ms pulses to 0-20 mV were recorded before and after the injection of the calcium buffer and the underlying Rrel was calculated. If the buffer concentration after the injection was high, the initial rate of rise of the calcium transient was slower after injection than before and was followed by a slow increase of [Ca2+] that resembled a ramp. The increase in myoplasmic [Mg2+] that accompanies the calcium transient in control was suppressed after the injection and a slight decrease was observed instead. After the injection the buffer concentration in the voltage-clamped segment of the fiber decreased as the buffer diffused away toward the open ends. The calculated apparent diffusion coefficient for fura-2 (Dapp = 0.40 +/- 0.03 x 10(-6) cm2/s, mean +/- SEM, n = 6) suggests that approximately 65-70% of the indicator was bound to relatively immobile intracellular constituents. As the concentration of the injected buffer decreased, the above effects were reversed. The changes in delta[Ca2+] were underlined by characteristic modification of Rrel. The early peak component was suppressed or completely eliminated; thus, Rrel rose monotonically to a maintained steady level if corrected for depletion. If Rrel was expressed as percentage of SR calcium content, the steady level after injection did not differ significantly from that before. Control injections of anisidine, to the concentration that eliminated the peak of Rrel when high affinity buffers were used, had only a minor effect on Rrel, the peak was suppressed by 26 +/- 5% (mean +/- SE, n = 6), and the steady level remained unchanged. Thus, the peak component of Rrel is dependent on a rise in myoplasmic [Ca2+], consistent with calcium-induced calcium release, whereas the steady component of Rrel is independent of myoplasmic [Ca2+].
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
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Megjelenés:Journal of General Physiology. - 101 : 2 (1993), p. 297-333. -
További szerzők:Jacquemond, Vincent Schneider, Martin F.
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11.

001-es BibID:BIBFORM024549
Első szerző:Csernoch László (élettanász)
Cím:Measurements of intracellular Mg2+ concentration in mouse skeletal muscle fibers with the fluorescent indicator Mag-Indo-1 / Laszlo Csernoch, Jean Claude Bernengo, Peter Szentesi, Vincent Jacquemond
Dátum:1998
ISSN:0006-3495
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:Biophysical Journal. - 75 (1998), p. 957-967. -
További szerzők:Bernengo, Jean Claude Szentesi Péter (1967-) (élettanász) Jacquemond, Vincent Szentesi Péter (1967-) (élettanász)
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12.

001-es BibID:BIBFORM004121
Első szerző:Csernoch László (élettanász)
Cím:Voltage-activated elementary calcium release events in isolated mouse skeletal muscle fibers / Laszlo Csernoch, Sandrine Pouvreau, Michel Ronjat, Vincent Jacquemond
Dátum:2008
Megjegyzések:The elementary Ca(2+)-release events underlying voltage-activated myoplasmic Ca(2+) transients in mammalian muscle remain elusive. Here, we looked for such events in confocal line-scan (x,t) images of fluo-3 fluorescence taken from isolated adult mouse skeletal muscle fibers held under voltage-clamp conditions. In response to step depolarizations, spatially segregated fluorescence signals could be detected that were riding on a global increase in fluorescence. These discrete signals were separated using digital filtering in the spatial domain; mean values for their spatial half-width and amplitude were 1.99 +/- 0.09 microm and 0.16 +/- 0.005 DeltaF/F(0) (n = 151), respectively. Under control conditions, the duration of the events was limited by the pulse duration. In contrast, in the presence of maurocalcine, a scorpion toxin suspected to disrupt the process of repolarization-induced ryanodine receptor (RyR) closure, events uninterrupted by the end of the pulse were readily detected. Overall results establish these voltage-activated low-amplitude local Ca(2+) signals as inherent components of the physiological Ca(2+)-release process of mammalian muscle and suggest that they result from the opening of either one RyR or a coherently operating group of RyRs, under the control of the plasma membrane polarization.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:The Journal of Membrane Biology. - 226 : (1-3) (2008), p. 43-55. -
További szerzők:Pouvreau, Sandrine Ronjat, Michel Jacquemond, Vincent
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