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1.

001-es BibID:BIBFORM004938
Első szerző:Balkay László (biofizikus)
Cím:Flow cytometric determination of intracellular free potassium concentration / Balkay, L., Marian, T., Emri, M., Krasznai, Z., Tron, L.
Dátum:1997
ISSN:0196-4763
Megjegyzések:A flow cytometric assay has been developed for determination of intracellular free potassium concentration ([K+]i). Investigated cells, loaded with the fluorescent pH indicator 2',7bis-(2-carboxyethyl)-5(and-6)-carboxyfluorescein (BCECF), are incubated in the presence of nigericin, and the intracellular pH is measured. The ionophore maintains the same ratio value [H+]/[K+] on both sides of the cytoplasmic membrane, so [K+]i can be evaluated from the measured intracellular pH (pHi) and the known parameters of the buffer. Application of the method revealed that the intracellular potassium concentration is significantly higher in lymphocytes than in proliferating cells of HUT-78, U266, and JY cell lines. A surprisingly low (60 mM) [K+]i concentration was observed with sperm cells of common carp. This method allows measurements on individual cells, provides data of excellent statistics, and still does not require large amounts of material. These features offer remarkable advantages over other techniques used for intracellular K+ measurements, such as steady-state fluorescence, atom absorption photometry, or energy-dispersive x-ray analysis.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
analysis
Animal
Biophysics
Buffers
Cell Line
Cells
diagnostic use
Dyes
Flow Cytometry
Fluorescein
Fluoresceins
Fluorescence
Fluorescent Dyes
Human
Hungary
Hydrogen-Ion Concentration
Ionophores
Lymphocytes
metabolism
Mice
Nigericin
Potassium
Rats
Megjelenés:Cytometry. - 28 : 1 (1997), p. 42-49. -
További szerzők:Márián Teréz (1950-) (radiobiológus) Emri Miklós (1962-) (fizikus) Krasznai Zoltán (1950-) (biofizikus) Trón Lajos (1941-) (biofizikus)
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2.

001-es BibID:BIBFORM031868
Első szerző:Emri Miklós (fizikus)
Cím:Temperature adaptation changes ion concentrations in spermatozoa and seminal plasma of common carp without affecting sperm motility / M. Emri, T. Márián, L. Trón, L. Balkay, Z. Krasznai
Dátum:1998
ISSN:0044-8486
Tárgyszavak:Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:Aquaculture. - 167 : 1-2 (1998), p. 85-94. -
További szerzők:Márián Teréz (1950-) (radiobiológus) Trón Lajos (1941-) (biofizikus) Balkay László (1963-) (biofizikus) Krasznai Zoltán (1950-) (biofizikus)
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3.

001-es BibID:BIBFORM031867
Első szerző:Emri Miklós (fizikus)
Cím:Wide applicability of a flow cytometric assay to measure absolute membrane potentials on the millivolt scale / Miklós Emri, László Balkay, Zoltán Krasznai, Lajos Trón, Teréz Márián
Dátum:1998
ISSN:0175-7571
Tárgyszavak:Természettudományok Fizikai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:European Biophysics Journal With Biophysics Letters. - 28 : 1 (1998), p. 78-83. -
További szerzők:Balkay László (1963-) (biofizikus) Krasznai Zoltán (1950-) (biofizikus) Trón Lajos (1941-) (biofizikus) Márián Teréz (1950-) (radiobiológus)
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4.

001-es BibID:BIBFORM004862
035-os BibID:(WOS)000229754600013 (scopus)19944422793
Első szerző:Fenyvesi Ferenc (gyógyszerész, gyógyszertechnológus)
Cím:Conformational and topological states of P-glycoprotein, an ABC transporter involved in multidrug resistance : novel assays of transporter modulation / Fenyvesi, F., Goda, K., Bacso, Z., Vecsernyes, M., Juhasz, I., Marian, T., Tron, L., Krasznai, Z., Szabo, G.
Dátum:2005
Tárgyszavak:Orvostudományok Elméleti orvostudományok idézhető absztrakt
folyóiratcikk
P-Glycoprotein
egyetemen (Magyarországon) készült közlemény
Megjelenés:European Journal of Pharmaceutical Sciences. - 25 : Suppl. 1 (2005), p. S16. -
További szerzők:Goda Katalin (1969-) (biofizikus) Bacsó Zsolt (1963-) (biofizikus) Vecsernyés Miklós (1959-) (gyógyszertechnológus, endokrinológus) Juhász István (1956-) (bőrgyógyász, bőrsebész, kozmetológus, klinikai onkológus) Márián Teréz (1950-) (radiobiológus) Trón Lajos (1941-) (biofizikus) Krasznai Zoltán (1950-) (biofizikus) Szabó Gábor (1953-) (biofizikus)
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5.

001-es BibID:BIBFORM006034
Első szerző:Gáspár Rezső (biofizikus)
Cím:Bretylium-induced voltage-gated sodium current in human lymphocytes / Rezső Gáspár, Zoltán Krasznai, Teréz Márián, Lajos Trón, Rina Recchioni, Marco Falasca, Fausto Moroni, Carlo Pieri, Sándor Damjanovich
Dátum:1992
ISSN:0167-4889
Megjegyzések:Using the whole-cell variation of the patch-clamp technique it has been determined that 0.25-3 mM bretylium tosylate (BT) exerts a repolarizing effect on partially depolarized human lymphocytes. The repolarizing effect was ouabain (40 microM)-sensitive, and was inhibited by the removal of external Na+ or by the Na(+)-channel-blocker amiloride (10-44 microM), but K(+)-channel-blockers 4-aminopyridine (0.1-5 mM) and quinine (100 microM) had no effect. The drug induced a sodium dependent, amiloride-sensitive transient inward current reaching its maximum value approx. 20-30 s after the administration of BT and lasting for 6-10 min. This current was activated by depolarization within 25 ms at around -42 mV, its inactivation took about 2 s and its reversal potential was +24 +/- 5 mV. An increase in the intracellular sodium concentration (1.8-3.2 mM) has been observed upon the addition of BT by monitoring the SBFI fluorescence of the dye-loaded cells. It has been shown that whole-cell K+ currents are significantly decreased by BT. The existence of voltage and ligand (BT)-gated sodium channels has been postulated in human lymphocytes. These channels are thought to participate in the initiation of membrane repolarization in human lymphocytes, and thereby influence mitogenic or antigen-induced cell-activation processes.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Bretylium
Ion channel
Patch-clamp
Human lymphocyte
Megjelenés:Biochimica et Biophysica Acta (BBA). Molecular Cell Research. - 1137 : 2 (1992), p. 143-147. -
További szerzők:Krasznai Zoltán (1950-) (biofizikus) Márián Teréz (1950-) (radiobiológus) Trón Lajos (1941-) (biofizikus) Recchioni, Rina Falasca, Marco Moroni, Fausto Pieri, Carlo Damjanovich Sándor (1936-2017) (biofizikus)
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6.

001-es BibID:BIBFORM002233
035-os BibID:WOS:000242995600010
Első szerző:Goda Katalin (biofizikus)
Cím:Complete inhibition of P-glycoprotein by simultaneous treatment with a distinct class of modulators and the UIC2 monoclonal antibody / Katalin Goda, Ferenc Fenyvesi, Zsolt Bacsó, Henrietta Nagy, Teréz Márián, Attila Megyeri, Zoltán Krasznai, István Juhász, Miklós Vecsernyés, Gábor Szabó
Dátum:2007
Megjegyzések:P-glycoprotein (Pgp) is one of the active efflux pumps that are able to extrude a large variety of chemotherapeutic drugs from the cells, causing multidrug resistance. The conformation-sensitive UIC2 monoclonal antibody potentially inhibits Pgp-mediated substrate transport. However, this inhibition is usually partial, and its extent is variable because UIC2 binds only to 10 to 40% Pgp present in the cell membrane. The rest of the Pgp molecules become recognized by this antibody only in the presence of certain substrates or modulators, including vinblastine, cyclosporine A (CsA), and SDZ PSC 833 (valspodar). Simultaneous application of any of these modulators and UIC2, followed by the removal of the modulator, results in a completely restored steady-state accumulation of various Pgp substrates (calcein-AM, daunorubicin, and 99mTc-hexakis-2- methoxybutylisonitrile), indicating near 100% inhibition of pump activity. Remarkably, the inhibitory binding of the antibody is brought about by coincubation with concentrations of CsA or SDZ PSC 833 20 times lower than what is necessary for Pgp inhibition when the modulators are applied alone. The feasibility of such a combinative treatment for in vivo multidrug resistance reversal was substantiated by the dramatic increase of daunorubicin accumulation in xenotransplanted Pgp tumors in response to a combined treatment with UIC2 and CsA, both administered at doses ineffective when applied alone. These observations establish the combined application of a class of modulators used at low concentrations and of the UIC2 antibody as a novel, specific, and effective way of blocking Pgp function in vivo.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
UIC2 monoclonal antibody
P-glycoprotein
Megjelenés:The Journal of Pharmacology and Experimental Therapeutics 320 : 1 (2007), p. 81-88. -
További szerzők:Fenyvesi Ferenc (1977-) (gyógyszerész, gyógyszertechnológus) Bacsó Zsolt (1963-) (biofizikus) Nagy Henrietta Márián Teréz (1950-) (radiobiológus) Megyeri Attila (1968-) (orvos) Krasznai Zoltán (1950-) (biofizikus) Juhász István (1956-) (bőrgyógyász, bőrsebész, kozmetológus, klinikai onkológus) Vecsernyés Miklós (1959-) (gyógyszertechnológus, endokrinológus) Szabó Gábor (1980-) (orvos) jr
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7.

001-es BibID:BIBFORM015620
Első szerző:Kovács É. (Gödöllő)
Cím:Quality of cryopreserved African catfish sperm following post-thaw storage / Kovacs, E., Muller, T., Marian, T., Krasznai, Z., Urbanyi, B., Horvath, A.
Dátum:2010
Megjegyzések:Experiments were carried out to test the fertilizing capacity and viability of cryopreserved African catfish (Clarias gariepinus) sperm following post-thaw storage at 4°C and following activation with water. In the first experiment, cryopreserved sperm was thawed and stored in a refrigerator for time periods ranging from 0 to 96 h before being used for fertilization. In all cases sperm stored for 24 h resulted in the highest fertilization percentages (37 ± 9%) which was significantly higher than that observed with sperm used for fertilization immediately after thawing (21 ± 4%, P < 0.05). These observations were later confirmed by flow cytometric assessment of membrane integrity of spermatozoa which showed an increase of the percentage of membrane-intact cells from 2 h post-thaw (66 ± 3%) to 26 h (77 ± 3%). In the second experiment, thawed and fresh sperm of African catfish was activated with water and used for fertilization at different time periods post-activation ranging from 0 to 120 s. The highest fertilization rate with cryopreserved sperm (72 ± 12%) was observed when eggs were fertilized with sperm activated for 20 s, however, sperm cells activated for 120 s were still able to fertilize eggs, albeit at a low rate (2 ± 3%). It was also noted that in the second trial of experiment 2 cryopreserved sperm resulted in significantly higher fertilization percentages than freshly extracted semen (P < 0.05).
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
ACTIVATION
Cells
FLOW
Semen
Spermatozoa
time
Water
OTKA::1
MAB::3.1
Megjelenés:Journal of Applied Ichthyology. - 26 : 5 (2010), p. 737-741. -
További szerzők:Müller T. (Gödöllő) Márián Teréz (1950-) (radiobiológus) Krasznai Zoltán (1950-) (biofizikus) Urbányi Béla (Gödöllő) Horváth A.
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8.

001-es BibID:BIBFORM054302
Első szerző:Krasznai Zoárd Tibor (szülész-nőgyógyász, gyermeknőgyógyász)
Cím:18FDG a PET tumor diagnostic tracer is not a substrate of the ABC transporter P-glycoprotein / Zoárd T. Krasznai, György Trencsényi, Zoltán Krasznai, Pál Mikecz, Enikő Nizsalóczki, Gábor Szalóki, Judit P. Szabó, László Balkay, Teréz Márián, Katalin Goda
Dátum:2014
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:European Journal of Pharmaceutical Sciences. - 64C (2014), p. 1-8. -
További szerzők:Trencsényi György (1978-) (biológus, biokémikus, molekuláris biológus) Krasznai Zoltán (1950-) (biofizikus) Mikecz Pál (1956-) (vegyész) Nizsalóczki Enikő Szalóki Gábor (1986-) (molekuláris biológus) Péli-Szabó Judit (1977-) (vegyész) Balkay László (1963-) (biofizikus) Márián Teréz (1950-) (radiobiológus) Goda Katalin (1969-) (biofizikus)
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9.

001-es BibID:BIBFORM046276
Első szerző:Krasznai Zoltán (biofizikus)
Cím:Flow cytometric determination of absolute membrane potential of cells / Krasznai Z., Marian T., Balkay L., Emri M., Tron L.
Dátum:1995
Megjegyzések:Membrane potential measurements using fluorescent membrane potential indicator dyes report on relative changes but usually do not result in an absolute value of the measured parameter. The method developed in this paper is based on the assumption that the negatively charged bis-oxonol distributes across the cytoplasmic membrane according to the Nernst equation. It is further supposed that the fluorescence intensity measured from a given stained cell is a single-value function of the intracellular dye concentration. The protocol suggested incorporates the construction of a calibration curve (fluorescence intensity measured from stained cells vs. extracellular dye concentration). This allows the evaluation of the membrane potential in millivolts using fluorescence readings of the cells both in the depolarized state and in the state of interest. Good agreement was found between absolute membrane potential data of human peripheral blood lymphocytes by our method and results of parallel patch clamp measurements.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:Journal of Photochemistry and Photobiology. B, Biology. - 28 : 1 (1995), p. 93-99. -
További szerzők:Márián Teréz (1950-) (radiobiológus) Balkay László (1963-) (biofizikus) Emri Miklós (1962-) (fizikus) Trón Lajos (1941-) (biofizikus)
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10.

001-es BibID:BIBFORM055409
Első szerző:Krasznai Zoltán (biofizikus)
Cím:In vivo visualization of multidrug resistance by parallel administration of 18FDG and 99MTC-MIBI / Z. Krasznai, N. Szincsák, B. Rubovszky, K. Goda, G. Szabó, I. Juhász, L. Galuska, K. Bálint, B. Dezső, L. Trón, T. Márián
Dátum:2000
ISSN:0340-6997
Tárgyszavak:Orvostudományok Elméleti orvostudományok idézhető absztrakt
Megjelenés:European Journal of Nuclear Medicine. - 27 (2000), p. 1181. -
További szerzők:Szincsák Nóra Rubovszky Bálint (1975-) (élettanász) Goda Katalin (1969-) (biofizikus) Szabó Gábor (1953-) (biofizikus) Juhász István (1956-) (bőrgyógyász, bőrsebész, kozmetológus, klinikai onkológus) Galuska László (1946-) (belgyógyász, izotópdiagnoszta) Bálint Klára Dezső Balázs (1951-) (pathológus) Trón Lajos (1941-) (biofizikus) Márián Teréz (1950-) (radiobiológus)
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11.

001-es BibID:BIBFORM040003
Első szerző:Krasznai Zoltán (biofizikus)
Cím:A slow outward current and a hypoosmolality induced anion conductance in embryonic chicken osteoclasts / Krasznai, Z., Weidema, F., Ypey, D. L., Damjanovich, S., Gaspar, R., Marian, T.
Dátum:2001
ISSN:0236-5383
Megjegyzések:In this paper we report on a hypoosmolality induced current, I(osmo), in embryonic chicken osteoclasts, which could only be studied when blocking a simultaneously active, unidentified slow outward current, I(slo). I(slo) was observed in all of the examined cells when both the intracellular and extracellular solutions contained sodium as the major cation and no potassium. The current was outwardly rectifying and activated at membrane potentials more positive than -44 +/- 12 mV (n = 31). The time to half activation of the current was also voltage dependent and was 350 ms at Vm = +80 mV, and 78 ms at Vm = +120 mV. The current did not inactivate during periods up to 5 s. Extracellular 4-AP (5 mM), TEA (5 mM) and Ba2+ (1 mM), blockers of K+ conductances in chicken osteoclasts, did not influence I(slo). However, I(slo) was inhibited by 50 microM extracellular verapamil, which allowed us to study I(osmo) in isolation. Exposure of the osteoclasts to hypotonic solution resulted in the development of a depolarization activated I(osmo). It developed after a 1-min delay and reached its maximum within 10 minutes. Half-maximal activation occurred after 4.4 +/- 0.9 min (n = 9). The current activated within a few ms upon depolarization and did not inactivate during at least 5 sec. I(osmo) reversed around the calculated Nernst potential for Cl- (E(Cl) = +7.3 mV and V(rev) = +5.4 +/- 3.6 mV, n = 9). The underlying conductance, G(osmo) exhibited moderate outward rectification around 0 mV in symmetrical Cl- solutions. Ion substitution experiments showed that G(osmo) is an anion conductance with P(Cl) approximately = P(F) > P(gluc) >> P(Na). I(osmo) was blocked by 0.5 mM SITS but 50 microM verapamil, 5 mM TEA, 5 mM 4-AP, 1 mM Ba2+, 50 microM cytochalasin D and 0.5 mM alendronate did not have any effect on the current. Cl- currents have been implicated in charge neutralization during osteoclastic acid secretion for bone resorption. The present results imply that osmolality may be a factor controlling this charge neutralization.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény hazai lapban
Animal
Anions
Calcium
Calcium Channel Blockers
Chick Embryo
Cytochalasin D
drug effects
Hungary
Ion Transport
Membrane Potentials
metabolism
Osmolar Concentration
Osteoclasts
pharmacology
physiology
Potassium
Sodium
Support,Non-U.S.Gov't
Verapamil
Megjelenés:Acta Biologica Hungarica. - 52 : 1 (2001), p. 47-61. -
További szerzők:Weidema, F. Ypey, Dirk L. Gáspár Rezső (1944-) (biofizikus) Márián Teréz (1950-) (radiobiológus) Damjanovich Sándor (1936-2017) (biofizikus)
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12.

001-es BibID:BIBFORM038939
Első szerző:Krasznai Zoltán (biofizikus)
Cím:Membrane hyperpolarization removes inactivation of Ca2+ channels, leading to Ca2+ influx and subsequent initiation of sperm motility in the common carp / Krasznai, Z., Marian, T., Izumi, H., Damjanovich, S., Balkay, L., Tron, L., Morisawa, M.
Dátum:2000
Megjegyzések:Change of osmolality surrounding spawned sperm from isotonic to hypotonic causes the initiation of sperm motility in the common carp. Here we show that membrane-permeable cAMP does not initiate motility of carp sperm that is quiescent in isotonic solution, and that motility of the demembranated sperm can be reactivated without cAMP. Furthermore, the cAMP level does not change during the initiation of sperm motility, and inhibitors of protein kinase do not affect sperm motility, suggesting that no cAMP-dependent system is necessary for the regulation of sperm motility. Sperm motility could not be initiated in Ca(2+)-free hypoosmotic solutions, and significant increase in the intracellular Ca(2+) level was observed by a Ca-sensitive fluorescence dye during hypoosmolality-induced active motion period. The demembranated sperm cells were fully reactivated in the solutions containing 10(-7) to 10(-5) M Ca(2+). Ca(2+) channel blockers such as verapamil and omega-conotoxin reversibly inhibited the initiation of sperm motility, suggesting that Ca(2+) influx is the prerequisite for the initiation of carp sperm motility. Motility of intact sperm was completely blocked; however, that of the demembranated sperm was not inhibited by the calmodulin inhibitor W7, suggesting that the calmodulin bound close to the plasma membrane participated in the initiation of sperm motility. Flow cytometric membrane potential measurements and spectrophotometric measurements by using fluorescence dyes showed transient membrane hyperpolarization on hypoosmolality-induced motility. This article discusses the role of membrane hyperpolarization on removal of inactivation of Ca(2+) channels, leading to Ca(2+) influx at the initiation of carp sperm motility.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:Proceedings of the National Academy of Sciences of the United States of America. - 97 : 5 (2000), p. 2052-2057. -
További szerzők:Márián Teréz (1950-) (radiobiológus) Izumi, Hiroko Damjanovich Sándor (1936-2017) (biofizikus) Balkay László (1963-) (biofizikus) Trón Lajos (1941-) (biofizikus) Morisawa, Masaaki
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