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001-es BibID:	BIBFORM046298
Első szerző:	Goda Katalin (biofizikus)
Cím:	Reversal of Multidrug Resistance by Valinomycin is Overcome by CCCP / Goda K., Krasznai Z., Gaspar R., Lankelma J., Westerhoff H. V., Damjanovich S., Szabo G.
Dátum:	1996
ISSN:	0006-291X
Megjegyzések:	Reversal of P-glycoprotein-mediated multidrug resistance by valinomycin is overcome by the proton ionophore, CCCP. This effect, a complete suppression of the 5- to 10-fold valinomycin-induced reversal ("re-reversal"), exhibits a sharp extracellular potassium concentration ([K+(0)]) dependence. It is observed at [K+(0)] > 2-4 mM and not at [K+(0)] greater than or equal to 2 mM, in the case of the fluorescent substrates rhodamine 123 and daunorubicin. The fact that "re-reversal" is detected only for the combination of CCCP with valinomycin raises the possibility that a direct interaction between these ionophores may explain the phenomenon. We show spectroscopic evidence of such an interaction, with a [K+(0)]-dependence similar to that of the "re-reversal." These data suggest that the reversal of P-glycoprotein activity by valinomycin can be compromised by anionic compounds such as CCCP due to complex formation. More generally, molecular interactions involving P-glycoprotein substrates or reversing agents may significantly affect drug accumulation in multidrug resistant cells.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:	Biochemical And Biophysical Research Communications. - 219 : 2 (1996), p. 306-310. -
További szerzők:	Krasznai Zoltán (1950-) (biofizikus) Gáspár Rezső (1944-) (biofizikus) Lankelma, Jan Westerhoff, Hans V. Damjanovich Sándor (1936-2017) (biofizikus) Szabó Gábor (1953-) (biofizikus)
Pályázati támogatás:	T14655 OTKA 17592 OTKA
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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001-es BibID:	BIBFORM046300
Első szerző:	Panyi György (biofizikus)
Cím:	Immunosuppressors inhibit voltage-gated potassium channels in human peripheral blood lymphocytes / Panyi G., Gaspar R., Krasznai Z., ter Horst J. J., Ameloot M., Aszalos A., Steels P., Damjanovich S.
Dátum:	1996
ISSN:	0006-291X
Megjegyzések:	The effects of immunosuppressive agents on the potassium current of human peripheral blood lymphocytes have been studied using the whole-cell patch-clamp technique. Cyclosporin A (10 micrograms/ml), rapamycin (10 micrograms/ml) and FK-506 (2.5 micrograms/ml) reduced the peak K+ current by approximately 40, 30 and 40% of the control, respectively, without any change in the reversal potential of the current. The current inhibition was similar at all membrane potentials studied and was accompanied with an increase in the rate of K+ current inactivation. Membrane potential measurements in current-clamp showed a marked depolarization of the membrane (>10 mV) upon the addition of either immunosuppressor to the cells. Our findings revealed that the voltage-dependent potassium current in human peripheral blood lymphocytes is inhibited by Cyclosporin A and other immunosuppressors, resulting in a depolarized membrane potential.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:	Biochemical and Biophysical Research Communications. - 221 : 2 (1996), p. 254-258. -
További szerzők:	Gáspár Rezső (1944-) (biofizikus) Krasznai Zoltán (1950-) (biofizikus) ter Horst, Jan J. Ameloot, Marcel Aszalos Adorján Steels, Paul Damjanovich Sándor (1936-2017) (biofizikus)
Pályázati támogatás:	1459 OTKA 1492 OTKA 6221 OTKA E12533 OTKA T14655 OTKA F13335 OTKA
Internet cím:	Intézményi repozitóriumban (DEA) tárolt változat
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