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1.

001-es BibID:BIBFORM037606
Első szerző:Hajdu Péter (biofizikus)
Cím:Answer to the "comment on functional consequences of Kv1.3 ion channel rearrangement into the immunological synapse" by Stefan Bittner et al. [Immunol. Lett. 125 (Aug 15 (2)) (2009) 156-157] / Hajdú, Péter, Szilágyi, Orsolya, Tóth, Ágnes, Krasznai, Zoltán, Pocsai, Krisztina, Panyi, György
Dátum:2010
ISSN:0165-2478
Tárgyszavak:Orvostudományok Elméleti orvostudományok szerkesztői levél
egyetemen (Magyarországon) készült közlemény
Megjelenés:Immunology Letters. - 129 : 1 (2010), p. 47-49. -
További szerzők:Szilágyi Orsolya (1985-) (molekuláris biológus, biokémikus) Tóth Ágnes (1983-) (biofizikus) Krasznai Zoltán (1950-) (biofizikus) Pocsai Krisztina (1978-) (élettanász) Panyi György (1966-) (biofizikus)
Internet cím:Intézményi repozitóriumban (DEA) tárolt változat
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DOI
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2.

001-es BibID:BIBFORM004884
Első szerző:Rubovszky Bálint (élettanász)
Cím:Detection of channel proximity by nanoparticle-assisted delaying of toxin binding : a combined patch-clamp and flow cytometric energy transfer study / Rubovszky, B., Hajdu, P., Krasznai, Z., Gaspar, R., Waldmann, T. A., Damjanovich, S., Bene, L.
Dátum:2005
ISSN:0175-7571
Megjegyzések:Gold nanoparticles of 30 nm diameter bound to cell-surface receptor major histocompatibility complex glycoproteins (MHCI and MHCII), interleukin-2 receptor alpha subunit (IL-2Ralpha), very late antigen-4 (VLA-4) integrin, transferrin receptor, and the receptor-type protein tyrosin phosphatase CD45 are shown by the patch-clamp technique to selectively modulate binding characteristics of Pi(2) toxin, an efficient blocker of K(v)1.3 channels. After correlating the electrophysiological data with those on the underlying receptor clusters obtained by simultaneously conducted flow cytometric energy transfer measurements, the modulation was proved to be sensitive to the density and size of the receptor clusters, and to the locations of the receptors as well. Based on the observation that engagement of MHCII by a monoclonal antibody down-regulates channel current and based on the close nanometer-scale proximity of the MHCI and MHCII glycoproteins, an analogous experiment was carried out when gold nanoparticles bound to MHCI delayed down-regulation of the K(v)1.3 current initiated by ligation of MHCII. Localization of K(v)1.3 channels in the nanometer-scale vicinity of the MHC-containing lipid rafts is demonstrated for the first time. A method is proposed for detecting receptor-channel or receptor-receptor proximity by observing nanoparticle-induced increase in relaxation times following concentration jumps of ligands binding to channels or to receptors capable of regulating channel currents.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Antibodies
Biophysics
Cell Line
chemistry
drug effects
Energy Transfer
Flow Cytometry
Glycoproteins
Gold
Human
Humans
Interleukin-2
Interleukin-2 Receptor alpha Subunit
Ion Channel Gating
Kv1.3 Potassium Channel
Ligands
Major Histocompatibility Complex
methods
Nanotubes
Particle Size
Patch-Clamp Techniques
pharmacokinetics
physiology
Potassium
Potassium Channels
Potassium Channels,Voltage-Gated
Protein Binding
Research
Scorpion Venoms
Support
T-Lymphocytes
Megjelenés:European Biophysics Journal. - 34 : 2 (2005), p. 127-143. -
További szerzők:Hajdu Péter (1975-) (biofizikus) Krasznai Zoltán (1950-) (biofizikus) Gáspár Rezső (1944-) (biofizikus) Waldmann, Thomas A. Damjanovich Sándor (1936-2017) (biofizikus) Bene László (1963-) (biofizikus)
Internet cím:elektronikus változat
DOI
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3.

001-es BibID:BIBFORM037605
Első szerző:Tóth Ágnes (biofizikus)
Cím:Functional consequences of Kv1.3 ion channel rearrangement into the immunological synapse / Ágnes Tóth, Orsolya Szilágyi, Zoltán Krasznai, György Panyi, Péter Hajdú
Dátum:2009
ISSN:0165-2478
Megjegyzések:Formation of immunological synapse (IS), the interface between T cells and antigen presenting cells, is a crucial step in T cell activation. This conjugation formation results in the rearrangement and segregation of a set of membrane bound and cytosolic proteins, including that of the T cell receptor, into membrane domains. It was showed earlier that Kv1.3, the dominant voltage-gated potassium channel of T cells redistributes into the IS on interaction with its specific APC. In the present experiments we investigated the functional consequences of the translocation of Kv1.3 channels into the IS formed between mouse helper T (T(h)2) and B cells. Biophysical characteristics of whole-cell Kv1.3 current in standalone cells (c) or ones in IS (IS) were determined using voltage-clamp configuration of standard whole-cell patch-clamp technique. Patch-clamp recordings showed that the activation of Kv1.3 current slowed (tau(a,1s) 2.36 +/- 0.13 ms (n = 7): tau(a,c) = 1.36 +/- 0.06 ms (n = 18)) whereas the inactivation rate increased (tau(i,1S) = 263 +/- 29 ms (n = 7): tau(i,c) = 365 +/- 27 ms (n = 17)) in cells being in IS compared to the standalone cells. The equilibrium distribution between the open and the closed states of Kv1.3 (voltage-dependence of steady-state activation) was shifted toward the depolarizing potentials in T cells engaged into IS (V-1/2,V-1S = -20.9 +/- 2 mV (n = 7). V-1/2,V-c = -26.4 +/- 115 mV (n = 12)). Thus, segregation of Kv1.3 channels into the IS modifies the gating properties of the channels. Application of protein kinase (PK) inhibitors (PKC: GF109203X, PKA: H89, p56Lck: damnacanthal) demonstrated that increase in the inactivation rate can be explained by the dephosphorylation of the channel protein. However, the slower activation kinetics of Kv1.3 in IS is likely to be the consequence of the redistribution of the channels into distinct membrane domains.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Cells
immunological synapse
Kinetics
mouse
Potassium
Proteins
egyetemen (Magyarországon) készült közlemény
Megjelenés:Immunology Letters. - 125 : 1 (2009), p. 15-21. -
További szerzők:Szilágyi Orsolya (1985-) (molekuláris biológus, biokémikus) Krasznai Zoltán (1950-) (biofizikus) Panyi György (1966-) (biofizikus) Hajdu Péter (1975-) (biofizikus)
Internet cím:Intézményi repozitóriumban (DEA) tárolt változat
DOI
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4.

001-es BibID:BIBFORM002770
Első szerző:Varga Zoltán (biofizikus, szakfordító)
Cím:Involvement of membrane channels in autoimmune disorders / Zoltán Varga, Péter Hajdu, György Panyi, Rezső Gáspár, Zoltán Krasznai
Dátum:2007
Megjegyzések:Ion channels are ubiquitous transmembrane proteins that are involved in a wide variety of cellular functions by selectively controlling the passage of ions across the plasma membrane. Among these functions many immune processes, including those in autoimmune reactions, also rely on the operation of ion channels, but the roles of ion channels can be very diverse. Here the participation of ion channels in three different roles in autoimmune processes is discussed: 1. ion channels in effector immune cells attacking other tissues causing autoimmune diseases, such as multiple sclerosis; 2. ion channels as direct targets of the immune system whereby loss of channel function leads to disease, as in myasthenia gravis; 3. ion channels whose function is modulated in the target cells by an apoptotic signal transduction cascade, such as the Fas/Fas ligand pathway. The numerous tasks that ion channels perform in autoimmune disorders and the wealth of information that has been gathered about them in recent years together provide a good basis for the design and production of drugs that may be effectively used in the therapy of these diseases
Tárgyszavak:Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
Animals
Autoimmune Diseases
Biophysics
Cell Membrane
Cells
Humans
Hungary
Immune System
Ion Channels
Lymphocyte Activation
metabolism
physiology
Proteins
Research
Signal Transduction
Support
T-Lymphocytes
therapy
Megjelenés:Current Pharmaceutical Design. - 13 : 24 (2007), p. 2456-2468. -
További szerzők:Hajdu Péter (1975-) (biofizikus) Panyi György (1966-) (biofizikus) Gáspár Rezső (1944-) (biofizikus) Krasznai Zoltán (1950-) (biofizikus)
Internet cím:elektronikus változat
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