Találati lista | Tudóstér

001-es BibID:	BIBFORM005095
Első szerző:	Shiba, K.
Cím:	Na+/Ca2+ exchanger modulates the flagellar wave pattern for the regulation of motility activation and chemotaxis in the ascidian spermatozoa / Shiba, K., Marian, T., Krasznai, Z., Baba, S. A., Morisawa, M., Yoshida, M.
Dátum:	2006
ISSN:	886-1544 (Print)
Megjegyzések:	Ion channels and ion exchangers are known to be important participants in various aspects of sperm physiology, e.g. motility activation, chemotaxis, the maintenance of motility and the acrosome reaction in the sperm. We report here on a role of the K+ -independent Na+/Ca2+ exchanger (NCX) on ascidian sperm. Reverse-transcriptase PCR reveals that the NCX is expressed in the testis while immunoblotting and immunolocalization demonstrate that the NCX exists on the sperm in the ascidian Ciona savignyi and C. intestinalis. A potent blocker of the NCX, KB-R7943 was found to block sperm-activating and -attracting factor (SAAF)-induced motility activation, sperm motility and sperm chemotaxis. We further analyzed the effects of this blocker on motility parameters such as the flagellar waveform, curvature, beat frequency, amplitude and wavelength of the sperm flagella. Inhibition of the NCX caused two distinct effects: a low concentration of KB-R7943 induced symmetric bending, whereas a high concentration of KB-R7943 resulted in asymmetric flagellar bending. These findings suggest that the NCX plays important roles in the regulation of SAAF-induced sperm chemotaxis, motility activation and motility maintenance in the ascidian. This study provides new information toward an understanding of Ca2+ transport systems in sperm motility and chemotaxis.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban analogs and derivatives Animals antagonists and inhibitors Anti-Arrhythmia Agents Calcium Calcium Channel Blockers Chemotaxis drug effects Immunoblotting Ion Channels Male metabolism pharmacology physiology Research Sodium-Calcium Exchanger Sperm Motility Sperm Tail Spermatozoa Support Thiourea Urochordata
Megjelenés:	Cell Motility and the Cytoskeleton. - 63 : 10 (2006), p. 623-632. -
További szerzők:	Márián Teréz (1950-) (radiobiológus) Krasznai Zoltán (1950-) (biofizikus) Baba, S. A. Morisawa, Masaaki Yoshida, Manabu
Internet cím:	DOI
Borító:
Saját polcon:

001-es BibID:	BIBFORM004330
Első szerző:	Yoshida, Kaoru
Cím:	Functional implications of membrane modification with semenogelins for inhibition of sperm motility in humans / Yoshida K., Krasznai Z. T., Krasznai Z., Yoshiike M., Kawano N., Yoshida M., Morisawa M., Tóth Z., Bazsáné Kassai Z., Márián T., Iwamoto T.
Dátum:	2009
Megjegyzések:	Semenogelin I and II (Sgs) are the major component of human semen coagulum. The protein is rapidly cleaved after ejaculation by a prostate-specific antigen, resulting in liquefaction of the semen coagulum and the progressive release of motile spermatozoa. Sgs inhibit human sperm motility; however, there is currently no information on its effect on the sperm membrane. This study investigated the role of Sgs on human sperm motility through regulation of membrane potential and membrane permeability. Fresh semen samples were obtained from normozoospermic volunteers, and studies were conducted using motile cells selected using the swim-up method. Sgs changed the characteristics of sperm motion from circular to straightforward as evaluated by a computer-assisted motility analyzer, and all parameters were decreased more than 2.5 mg/mL. The results demonstrate that Sgs treatment immediately hyperpolarized the membrane potential of swim-up-selected sperm, changed the membrane structure, and time-dependently increased membrane permeability, as determined through flow cytometric analysis. The biphasic effects of Sgs were time- and dose-dependent and partially reversible. In addition, a monoclonal antibody against Sgs showed positive binding to cell membrane proteins in fixed cells, observed with confocal fluorescence microscopy. These results demonstrate that Sgs modifies the membrane structure, indirectly inhibiting motility, and provides suggestions for a therapy for male infertility through selection of a functional sperm population using Sgs.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:	Cell Motility and the Cytoskeleton 66 : 2 (2009), p. 99-108. -
További szerzők:	Krasznai Zoárd Tibor (1973-) (szülész-nőgyógyász, gyermeknőgyógyász) Krasznai Zoltán (1950-) (biofizikus) Yoshiike, Miki Kawano, Natsuko Yoshida, Manabu Morisawa, Masaaki Tóth Zoltán (1948-) (szülész-nőgyógyász, humángenetikus) Bazsáné Kassai Zsuzsa (1946-) (embriológus) Márián Teréz (1950-) (radiobiológus) Iwamoto, Teruaki
Internet cím:	elektronikus változat DOI
Borító:
Saját polcon: