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001-es BibID:BIBFORM076595
Első szerző:Sipka Sándor (laboratóriumi szakorvos)
Cím:Effects of L-amino Acids on Human Peripheral Neutrophil Granulocyte Activation / Sipka Sándor, Keresztes Tamás, Kovács Ildikó, Sipka Sándor Jr., Baráth Sándor, Szegedi Gyula, Zeher Margit, Bruckner Geza
Dátum:2014
Megjegyzések:Objective: The objective was to investigate the early (20 minutes) effects of 21 L-amino acids on the activation of human neutrophils and to determine in healthy individuals the effects of a meal on the 1) number and relative luminescence unit (RLU) of peripheral neutrophils, 2) serum glutamate and glucose levels and 3) mTOR signaling network. Methods: The RLU of neutrophils stimulated by Ca2+ ionophor (CaI) and phorbol myristate acetate (PMA) following amino acid supplementation (3 x 10-4 M) or after consuming a meal was determined. L-glutamate was measured by HPLC. Results: All amino acids resulted in significant inhibitions of neutrophil RLU, except for arginine, which stimulated neutrophils. The ratios of amino acid induced inhibition were significantly higher in the cells stimulated by PMA than by CaI. The consumption of a meal resulted in a significant serum glutamate elevation compared to baseline (2.3 versus 0.9 x10-4 M) 90 minutes after ingestion of the meal. It was independent of the body mass index and returned near fasting levels after 150 minutes. The number of neutrophils was significantly elevated 90 minutes after the meal but the PMA induced RLU was significantly decreased. Conclusion: Our ex vivo and in vivo results suggest that the L-amino acids, independent of their metabolic significance, may continuosly and quickly modify the activity of human peripheral neutrophils, and also the outcome of various immunologic reactions. The activation of the mTORC1 complex likely involves a transient impairment in the function of mTORC2 complex in these processes.
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
L-amino acids
L-glutamate
mTOR
neutrophils
RLU
Megjelenés:The Open Nutrition Journal. - 8 (2014), p. 1-7. -
További szerzők:Keresztes Tamás Kovács Ildikó Sipka Sándor ifj. (1980-) (orvos) Baráth Sándor (1977-) (biológus) Szegedi Gyula (1936-2013) (belgyógyász, immunológus) Zeher Margit (1957-2018) (belgyógyász, allergológus és klinikai immunológus, reumatológus) Bruckner Géza
Pályázati támogatás:71883
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