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001-es BibID:	BIBFORM024608
Első szerző:	Rezes Szilárd (fül-orr-gégész)
Cím:	Human bocavirus and rhino-enteroviruses in childhood otitis media with effusion / Rezes S., Söderlund-Venermo M., Roivainen M., Kemppainen K., Szabó Z., Sziklai I., Pitkäranta A.
Dátum:	2009
Megjegyzések:	Viral respiratory infections play an important role in the pathogenesis of otitis media with effusion (OME) in children. The most common human rhinoviruses (HRVs) have been detected in middle ear effusions (MEE), but there is only limited data available about the closely related human enteroviruses (HEVs). The newly discovered human bocavirus (HBoV) has not, however, been identified in MEE of OME children. OBJECTIVES: The aim of our study was to determine the presence of HBoV and HRV/HEV and the rate of coinfection in a set of MEE samples collected from OME children. STUDY DESIGN: Seventy-five MEE samples from 54 children with no acute respiratory symptoms were studied with reverse transcription polymerase chain reaction (RT-PCR) for detection of HRV/HEV and quantitative PCR for detection of HBoV. RESULTS: Twenty-six (35%) of 75 MEE samples were positive for viral nucleic acid, 22 (29%) for HEV, 10 (13%) for HRV and 2 (3%) for HBoV. There was no statistically significant difference between mucoid and serous effusions in the rate of virus detection. Forty-three percent of bilateral cases showed a contra-lateral difference in viral finding. CONCLUSIONS: Our results suggest that these common respiratory viruses can be associated with OME in children. Whether these viruses are causative etiologic factors of MEE persistence or merely remnants of previous infections is not known.
Tárgyszavak:	Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:	Journal of Clinical Virology. - 46 : 3 (2009), p. 234-237. -
További szerzők:	Söderlund-Venermo, Maria Roivainen, Merja Kemppainen, Kaisa Szabo Zsolt Sziklai István (1954-) (fül-orr-gégész) Pitkäranta, Anne
Internet cím:	Intézményi repozitóriumban (DEA) tárolt változat
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001-es BibID:	BIBFORM040769
Első szerző:	Szabo Zsolt
Cím:	A detailed procedure and dissection guide for the isolation of spiral ganglion cells of the guinea pig for electrophysiological experiments / Szabó, Zs., Harasztosi, Cs., Szűcs, G., Sziklai, I., Rusznák, Z.
Dátum:	2003
ISSN:	1385-299X
Megjegyzések:	In the present study step-by-step instructions are provided for a preparative technique employed for the removal of the spiral ganglion from the inner ear of the guinea pig. Removal of the temporal bone is followed by opening of the bulla and excision of the modiolus. All major steps of the technique are illustrated with photographs. A procedure to obtain surviving, acutely separated spiral ganglion neurones is also described. By this procedure small tissue pieces are removed from the modiolus which contain the spiral ganglion neurones. The tissue fragments then undergo a mild enzyme treatment (collagenase and pronase). After the enzyme exposure, the tissue pieces are gently triturated, and the isolated cells are allowed to settle. Poly-D-lysine ensured the firm attachment of the spiral ganglion cells to the cover-slips. The application of this adhesive coating seemed to be desirable in functional studies when microelectrode techniques and/or rapid exchange of the extracellular solution were employed.
Tárgyszavak:	Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:	Brain Research Protocols. - 10 : 3 (2003), p. 139-147. -
További szerzők:	Harasztosi Csaba Szűcs Géza (1948-) (élettanász) Sziklai István (1954-) (fül-orr-gégész) Rusznák Zoltán (1965-) (élettanász)
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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001-es BibID:	BIBFORM039760
035-os BibID:	PMID:12453052
Első szerző:	Szabo Zsolt
Cím:	Ionic currents determining the membrane characteristics of type I spiral ganglion neurons of the guinea pig / Zs. Szabó, Cs. Harasztosi, I. Sziklai, G. Szűcs, Z. Rusznák
Dátum:	2002
ISSN:	0953-816X
Megjegyzések:	Enzymatically isolated type I spiral ganglion neurons of the guinea pig have been investigated in the present study. The identity of the cells was confirmed by using anti-neuron-specific enolase immunostaining. The presence and shredding of the myelin sheath was also documented by employing anti-S100 immunoreaction. The membrane characteristics of the cells were studied by using the whole-cell patch-clamp technique. The whole-cell capacitance of the cells was 9 +/- 2 pF (n = 51), while the resting membrane potential of the cells was -62 +/- 9 mV (n = 19). When suprathreshold depolarizing stimuli were applied, the neurons fired a single action potential at the beginning of the stimulation. It was confirmed in this study that type I spiral ganglion cells possess a hyperpolarization-activated nonspecific cationic current (Ih). The major characteristics of this current component were unaffected by the enzyme treatment. Type I spiral ganglion cells also expressed various depolarization-activated K+ current components. A high-threshold outward current was sensitive to 1-10 mm TEA+ application. The ganglion cells also expressed a relatively small, but nevertheless present, transient outward current component which was less sensitive to TEA+ but could be inhibited by 100 micro m 4-aminopyridine. A DTX-I-sensitive current was responsible for some 30% of the total outward current (at 0 mV), showed rapid activation at membrane potentials positive to -50 mV and demonstrated very little inactivation. However, inhibition of the highly 4-AP- or DTX-I-sensitive component did not alter the rapidly inactivating nature of the firing pattern of the cells.
Tárgyszavak:	Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban egyetemen (Magyarországon) készült közlemény
Megjelenés:	European Journal of Neuroscience. - 16 : 10 (2002), p. 1887-1895. -
További szerzők:	Harasztosi Csaba Sziklai István (1954-) (fül-orr-gégész) Szűcs Géza (1948-) (élettanász) Rusznák Zoltán (1965-) (élettanász)
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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