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001-es BibID:	BIBFORM045517
Első szerző:	Szőnyi Magdolna
Cím:	Acetylcholine-induced phosphorlylation in isolated outer hair cells / Szőnyi, M., Csermely, P., Sziklai, I.
Dátum:	1999
ISSN:	0001-6489
Megjegyzések:	Two groups of isolated, surviving outer hair cells (OHCs) of guinea pig cochleas (n = 20, for each group) were treated with 10 microM acetylcholine or acetylcholine plus strichnine (an alpha9 nAChR antagonist), respectively, under short-term tissue culture conditions. The protein content of the cell homogenates was separated by SDS-polyacrylamide gel electrophoresis, Western blotted and labelled with an antibody against phosphoserine residues. Signals were detected using the ECL system. Acetylcholine challenge of the OHCs resulted in a difference in the pattern of phosphorylated proteins from those of strichnine pretreated cells. A 220 kDa and a 120 kDa protein expressed a more intense phosphorylated state in the ACh group compared with the ACh plus strichnine group. The 220 kDa phosphoprotein is in the range of the cytoskeletal protein beta-fodrin, whereas the 120 kDa fraction is similar to alpha-fodrin or an ankyrin isoform. Phosphorylation of proteins due to activation of the AChR by agonist can play a role in the signalling mechanism between receptor activation and increase in the electromotile capability of isolated OHCs.
Tárgyszavak:	Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:	Acta Oto-Laryngologica. - 119 : 2 (1999), p. 185-188. -
További szerzők:	Csermely Péter Sziklai István (1954-) (fül-orr-gégész)
Internet cím:	Intézményi repozitóriumban (DEA) tárolt változat
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